Search CTL/CD8+ T-Cell Epitope Database

Found 12 matching records:

Displaying record number 60108

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	Env(419-427)
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	RW9
Species (MHC/HLA)	human(A*32)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RIKQIINMW had a significantly positive association with HLA A*32; it was recognized by 7/22 A*32-positive subjects and 10/230 A*32-negative subjects.
RIKQIINMW was significantly protective, associated with viral control when restricted by HLA-A*32 in a cohort of 245 controllers and 96 progressors.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 55956

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	RW9(gp120)
Subtype	B
Species (MHC/HLA)	human(A32)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Prewviously described HLA-A32-restricted epitope RIKQIINMW elicited an immune response in Chinese HIV-1 positive subjects as part of peptides ENITLPCRIKQIINMW and CRIKQIINMWQEVGKAMY.

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 793

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120(420-428)
Epitope	`RIKQIINMW`	Epitope Alignment
Species (MHC/HLA)	human(A32)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

This epitope is processed by a TAP1/2 dependent mechanism.

References

Ferris1999 R. L. Ferris, C. Hall, N. V. Sipsas, J. T. Safrit, A. Trocha, R. A. Koup, R. P. Johnson, and R. F. Siliciano. Processing of HIV-1 Envelope Glycoprotein for Class I-Restricted Recognition: Dependence on TAP1/2 and Mechanisms for Cytosolic Localization. J. Immunol., 162:1324-1332, 1999. PubMed ID: 9973386. Show all entries for this paper.

Displaying record number 52277

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	A32-RW10(gp120)
Subtype	B
Species (MHC/HLA)	human(A32)
Immunogen	HIV-1 infection
Patient MHC/HLA	A32, B44; A30, A32, B18, B27
Experimental methods
Keywords	HAART, ART, supervised treatment interruptions (STI)

Notes

Peripheral blood (PB) and lymph node (LN) CD8+ T-cell responses were compared in 15 asymptomatic HIV-1 infected patients using all known optimal CTL epitopes (http://hiv-web.lanl.gov/content/hiv-db/REVIEWS/brander2001.html) for each person's class I HLA alleles.
60 epitope responses were detected in both PB and LN samples of the 15 patients, and an additional 8 responses were detected only in LN. The total magnitude of the response was similar in LN and PB, but the percentage of CD8+ T cells in the LN is lower so the number of HIV-specific cells per million CD8+ T-cells is higher in the LN.
1 year post-HAART treatment in five patients studied, the magnitude of the CD8 T-cell response was decreased in both LN and PB, but more dramatically in PB, and 13/25 epitope responses in the PB became undetectable, in contrast to 5/26 in the LN.
Treatment interruption following HAART induced resulted in increased viremia accompanied by the restoration of the detection of 13 epitopes that had become undetectable in the PB, and the addition of 9 novel epitope responses.
Breakdowns of epitope responses were shown for 4 individuals. Patient B displayed the greatest response to epitope B44-AW11(p24) and also responded to A32-PW10(RT) in both PB and LN samples, while a third response against epitope A32-RW10(gp120) was only detected in the LN sample. Patient D displayed the greatest response to B27-KK10 (p24), and also responded to A30-RY11(p17), A32-PW10(RT), A30-KY11(RT), A32-RW10(gp120), and B18-YY9(Nef).

References

Altfeld2002 Marcus Altfeld, Jan van Lunzen, Nicole Frahm, Xu G. Yu, Claus Schneider, Robert L. Eldridge, Margaret E. Feeney, Dirk Meyer-Olson, Hans-Juergen Stellbrink, and Bruce D. Walker. Expansion of Pre-existing, Lymph Node-localized CD8+ T Cells During Supervised Treatment Interruptions in Chronic HIV-1 Infection. J. Clin. Invest., 109(6):837-843, Mar 2002. PubMed ID: 11901192. Show all entries for this paper.

Displaying record number 53730

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	Env(424-432 BRU)
Epitope	`RIKQIINMW`	Epitope Alignment
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(A32)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons

Notes

CD8+ T-cells from HIV-1 CRF02_AG-infected Ivorians could recognize clade B epitopes. No difference was observed in the number of recognized peptide pools between nine French subtype B infected study subjects, and nine CRF02_AG infected Ivorian subjects.
This epitope was recognized by 0/9 CRF02_AG-infected Ivorians, and 3/9 B-infected French subjects.

References

Inwoley2005 André Inwoley, Patricia Recordon-Pinson, Marion Dupuis, Jessintha Gaston, Mathieu Genête, Albert Minga, Franck Letourneur, François Rouet, Jeannine Choppin, Hervé Fleury, Jean-Gérard Guillet, Muriel Andrieu, and ANRS 1220 PRIMO-CI Study Group. Cross-Clade Conservation of HIV Type 1 Nef Immunodominant Regions Recognized by CD8+ T Cells of HIV Type 1 CRF02\_AG-Infected Ivorian (West Africa). AIDS Res. Hum. Retroviruses, 21(7):620-628, Jul 2005. PubMed ID: 16060833. Show all entries for this paper.

Displaying record number 791

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120(424-432 HXB2)
Epitope	`RIKQIINMW`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A*32:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	15160: A03, A32, B14, B44
Country	United States
Experimental methods	Chromium-release assay
Keywords	autologous responses, novel epitope

Notes

C. Brander notes that this is an A*3201 epitope in the 1999 database.
A long term non-progressor (LTNP) was studied for detailed analyses of CTL response to epitopes correlated to the subject's viral quasispecies. Epitope mapping revealed CTL response to 6 epitopes in p17, p24, RT, Env and Nef. Results show that (a) multiple epitopes were targeted simultaneously (b) viral heterogeneity/antigenic diversity is not necessary for such a multispecific CTL response (c) LTNP infection does not need to be associated with unique epitope targeting as 5/6 epitopes targeted have been seen in other subjects with disease progression (d) HIV-1-specific CTL can be highly active, disallowing viral escapes, but broadly cross-reactive (recognizing known variants) even with low viral load (VL).
A Gag-specific clone, VE7, from the LTNP subject 15160 recognized a gp120, HLA-A*32-restricted novel epitope, RIKQIINMW, which overlaps previously identified RAEQASQEV. 9 aa. RIKQIINMW is the optimal epitope.

References

Harrer1996b T. Harrer, E. Harrer, S. A. Kalams, P. Barbosa, A. Trocha, R. P. Johnson, T. Elbeik, M. B. Feinberg, S. P. Buchbinder, and B. D. Walker. Cytotoxic T Lymphocytes in Asymptomatic Long-Term Nonprogressing HIV-1 Infection. Breadth and Specificity of the Response and Relation to In Vivo Viral Quasispecies in a Person with Prolonged Infection and Low Viral Load. J. Immunol., 156:2616-2623, 1996. PubMed ID: 8786327. Show all entries for this paper.

Displaying record number 1136

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120(419-427 HXB2)
Epitope	`RIKQIINMW`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*32:01)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is an A*3201 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 56254

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location
Epitope	`RIKQIINMW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*32:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	A32:04, A74:12, B07:02, B44:03, C02:10, C07:02
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression

Notes

47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
Known epitope RIKQIINMW is HLA-A*3201-restricted. Response to a peptide containing this epitope was detected in an HLA-A*3204 positive rapid progressor, 12 weeks post-infection.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 60107

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	Env(419-427)
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	RW9
Species (MHC/HLA)	human(C*06)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RIKQIINMW had a significantly positive association with HLA C*06; it was recognized by 46/89 C*06-positive subjects and 17/252 C*06-negative subjects.
RIKQIINMW was significantly protective, associated with viral control when restricted by HLA-C*06 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 60109

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	Env(419-427)
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	RW9
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RIKQIINMW had a significantly positive association with HLA C*12; it was recognized by 5/36 C*12-positive subjects and 5/194 C*12-negative subjects.
RIKQIINMW was significantly protective, associated with viral control when restricted by HLA-C*12 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 57675

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	Env
Epitope	`RIKQIINMW`	Epitope Alignment
		Show epitope variants
Epitope Name	EnvRW9
Subtype	A, B, C, D
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	Subject A: A01, A26, B56:01, B57, C01, C06
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	subtype comparisons, vaccine-induced immune responses

Vaccine Details

Vaccine type	DNA prime with Ad5 boost
Vaccine strain	A clade, B clade, C clade

Notes

A DNA/rAD vaccine induced greater HIV-1-specific CD8+ T-cell responses and higher antibody titers, compared to either a DNA or rAD vaccine alone. This boost in recognition increased the frequency of CTL response, but did not affect either functional avidity or recognition pattern much. Epitopes mapped in Gag and Env demonstrated partial cross-clade recognition.
1/9 vaccinees (Subject A) had a positive response to this epitope in EliSpot assay. The minimum epitope was determined from the original 15-mer. It was shown to be a CD8 epitope by ICS assay.
Before boost, the responding subject had an EliSpot response to the wild-type sequence (variants not tested). After boost, the subject responded with greater frequency to the wild-type and variants kIKQIINMW and RIKQIINrW.

References

Koup2010 Richard A. Koup, Mario Roederer, Laurie Lamoreaux, Jennifer Fischer, Laura Novik, Martha C. Nason, Brenda D. Larkin, Mary E. Enama, Julie E. Ledgerwood, Robert T. Bailer, John R. Mascola, Gary J. Nabel, Barney S. Graham, VRC 009 Study Team, and VRC 010 Study Team. Priming Immunization with DNA Augments Immunogenicity of Recombinant Adenoviral Vectors for Both HIV-1 Specific Antibody and T-Cell Responses. PLoS One, 5(2):e9015, 2010. PubMed ID: 20126394. Show all entries for this paper.

Displaying record number 57168

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HXB2 Location	gp160(419-427) gp120(419-427) DNA(7479..7505)	gp160 Epitope Map
Author Location	gp120(419-427)
Epitope	`RIKQIINMW`	Epitope Alignment
Epitope Name	RW9
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	A26, A32, B27, Cw1, Cw2
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HIV exposed persistently seronegative (HEPS), acute/early infection

Notes

CTL responses were measured in a highly exposed patient who was seronegative for 2 years, both before and after seroconversion. CTL responses after seroconversion were stronger, and more of the responses were associated with protective HLA allele B27.
RIKQIINMW was part of a pool that was not recognized by the HEPS patient before seroconversion; it elicited an EliSpot response after seroconversion. The patient's consensus sequence at day 24 after seroconversion was the same as the test peptide.

References

Liu2009b Yi Liu, Amanda Woodward, Haiying Zhu, Thomas Andrus, John McNevin, Jean Lee, James I. Mullins, Lawrence Corey, M. Juliana McElrath, and Tuofu Zhu. Preinfection Human Immunodeficiency Virus (HIV)-Specific Cytotoxic T Lymphocytes Failed to Prevent HIV Type 1 Infection from Strains Genetically Unrelated to Viruses in Long-Term Exposed Partners. J. Virol., 83(20):10821-10829, Oct 2009. PubMed ID: 19706711. Show all entries for this paper.