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Displaying record number 770

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HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location	Env
Epitope	`RGPGRAFTVTI`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2D^d)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Vaccine type	DNA
Vaccine component	Env V3

Notes

Vaccinated mice elicited a CTL response to a gene gun-delivered multiepitope vaccine to two epitopes studied that are known to elicit CTL in mice: SYIPSAEKI from Plasmodium berghei and RGPGRAFTVTI from HIV-1 Env.
Different vaccination protocols were tested and it was found that a gene gun mediated delivery followed by an MVA boost was as good as i. m. immunization followed by a MVA boost -- this is advantageous as gene gun delivery requires far less DNA than i.m. DNA priming.
CTL activity was high (60% - 70% specific lysis at effector target) when vaccinated with a single gene gun immunization and an MVA boost, and improved with two gene gun vaccinations.

References

Hanke1999 T. Hanke and A. McMichael. Pre-Clinical Development of a Multi-CTL Epitope-Based DNA Prime MVA Boost Vaccine for AIDS. Immunol. Lett., 66:177-181, 1999. PubMed ID: 10203052. Show all entries for this paper.

Hanke1999a T. Hanke, V. C. Neumann, T. J. Blanchard, P. Sweeney, A. V. Hill, G. L. Smith, and A. McMichael. Effective Induction of HIV-Specific CTL by Multi-Epitope Using Gene Gun in a Combined Vaccination Regime. Vaccine, 17:589-596, 1999. PubMed ID: 10075166. Show all entries for this paper.