Search CTL/CD8+ T-Cell Epitope Database

Found 15 matching records:

Displaying record number 58495

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag(294-)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A24, A26, B44, B70)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-B*A*24/A*26/B*44/B*70-restricted Gag optimal epitope RDYVDRFYKTL was tested in a dual color EliSpot and found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Peretz2011 Yoav Peretz, Olivia Marra, Réjean Thomas, Danielle Legault, Pierre Côté, Mohamed-Rachid Boulassel, Danielle Rouleau, Jean-Pierre Routy, Rafick-Pierre Sékaly, Christos M Tsoukas, Cécile Tremblay, and Nicole F. Bernard. Relative Contribution of HIV-Specific Functional Lymphocyte Subsets Restricted by Protective and Non-Protective HLA Alleles. Viral Immunol, 24(3):189-198, Jun 2011. PubMed ID: 21668360. Show all entries for this paper.

Displaying record number 57207

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	(A24)
Immunogen	HIV-1 infection
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	mother-to-infant transmission

Notes

HIV-1 peptide-specific IFN-γ responses were measured over the first year of life for vertically-infected infants. Late-infected infants (between months 1 and 12) had responses that were more rapid and more focused than those infected before 1 month of life.
1/2 infants tested recognized RDYVDRFY/FKTL.
Late-infected infants directed strong responses to conserved epitopes in the major homology region of Gag; such responses were rare in early-infected infants.
Variants of this peptide were RDYVDRFY/FKTL (RDYVDRFyKTL and RDYVDRFfKTL)

References

Lohman-Payne2009 B. Lohman-Payne, J. A. Slyker, B. A. Richardson, C. Farquhar, M. Majiwa, E. Maleche-Obimbo, D. Mbori-Ngacha, J. Overbaugh, S. Rowland-Jones, and G. John-Stewart. Infants with Late Breast Milk Acquisition of HIV-1 Generate Interferon-gamma Responses More Rapidly than Infants with Early Peripartum Acquisition. Clin. Exp. Immunol., 156(3):511-517, Jun 2009. PubMed ID: 19438605. Show all entries for this paper.

Displaying record number 314

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(293-312 LAI)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A26, B44, B70)
Immunogen	HIV-1 infection
Experimental methods
Keywords

References

Ogg1998b G. S. Ogg, T. Dong, P. Hansasuta, L. Dorrell, J. Clarke, R. Coker, G. Luzzi, C. Conlon, A. P. McMichael, and S. Rowland-Jones. Four Novel Cytotoxic T-Lymphocyte Epitopes in the Highly Conserved Major Homology Region of HIV-1 Gag, Restricted through B*4402, B*1801, A*2601, B*70 (B*1509). AIDS, 12:1561-1563, 1998. PubMed ID: 9727585. Show all entries for this paper.

Displaying record number 60097

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag(294-304)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Epitope Name	RL11
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RDYVDRFYKTL had a significantly positive association with HLA B*18; it was recognized by 3/22 B*18-positive subjects and 6/269 B*18-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 58535

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(162-172)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B18, B44, B*70)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-B*18/B*2601/B*44/B*70-restricted p24 optimal epitope RDYVDRFYKTL was tested in a dual color EliSpot and was found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Displaying record number 57713

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	human(B*44)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	supervised treatment interruptions (STI), escape

Notes

Chronically infected HIV-1-infected patients were examined during treatment interruption (TI) for associations between viral control and presence of escape mutations. Patients 311 and 313 who had best viral control exhibited CTL response against at least 1-2 epitopes early in TI, followed by increasing numbers of epitopes recognized and increasing mutations developed in time . In patient 410 with worst viral load control only 2 epitopes elicited CTL response through all of TI, and no mutations were detected.
Epitope RDYVDRFYKTL elicited a CTL response from week 48 after start of TI in Patient 313; no mutants were found to develop.

References

Schweighardt2010 Becky Schweighardt, Terri Wrin, Duncan A. Meiklejohn, Gerald Spotts, Christos J. Petropoulos, Douglas F. Nixon, and Frederick M. Hecht. Immune Escape Mutations Detected within HIV-1 Epitopes Associated with Viral Control During Treatment Interruption. J. Acquir. Immune Defic. Syndr., 53(1):36-46, Jan 2010. PubMed ID: 19910798. Show all entries for this paper.

Displaying record number 1806

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(162-172)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	human(B*44)
Immunogen	HIV-1 infection
Experimental methods
Keywords	rate of progression, acute/early infection

Notes

The CTL response to optimally defined CTL epitopes restricted by HLA class I A and B alleles in individuals who coexpressed HLA A*02, A*03, and B*07 was studied in eight HIV-1-infected subjects, two with acute infection, five with chronic, and one long-term non-progressor (LTNP).
2 to 17 epitopes were recognized in a given individual, A*02-restricted CTL response tended to be narrow and never dominated the response. 25/27 epitopes were targeted by at least one person.
RDYVDRFYKTL was one of the B*44-restricted epitopes tested for responses in this investigation.

References

Day2001 C. L. Day, A. K. Shea, M. A. Altfeld, D. P. Olson, S. P. Buchbinder, F. M. Hecht, E. S. Rosenberg, B. D. Walker, and S. A. Kalams. Relative dominance of epitope-specific cytotoxic T-lymphocyte responses in human immunodeficiency virus type 1-infected persons with shared HLA alleles. J. Virol., 75(14):6279-91, Jul 2001. URL: http://jvi.asm.org/cgi/content/full/75/14/6279. PubMed ID: 11413294. Show all entries for this paper.

Displaying record number 54371

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Epitope Name	B44-RL11(p24)
Species (MHC/HLA)	human(B44)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contributions of immune responses restricted to individual HLA class I molecules were studied.
This epitope-HLA combination was recognized. The most frequently recognized epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contributions of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 1457

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(162-172)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	human(B44)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

One of the 51 HIV-1 epitopes selected by Ferrari et al. as good candidate CTL epitopes for vaccines by virtue of being conserved and presented by common HLA alleles.

References

Ferrari2000 G. Ferrari, D. D. Kostyu, J. Cox, D. V. Dawson, J. Flores, K. J. Weinhold, and S. Osmanov. Identification of highly conserved and broadly cross-reactive HIV type 1 cytotoxic T lymphocyte epitopes as candidate immunogens for inclusion in Mycobacterium bovis BCG-vectored HIV vaccines. AIDS Res. Hum. Retroviruses, 16(14):1433-43, 20 Sep 2000. PubMed ID: 11018863. Show all entries for this paper.

Displaying record number 52426

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Subtype	B, D
Species (MHC/HLA)	human, macaque(B44)
Immunogen	HIV-1 infection, vaccine
Experimental methods
Keywords	subtype comparisons, epitope processing, vaccine-induced immune responses, immunodominance

Vaccine Details

Vaccine type	DNA prime with modified vaccinia Ankara (MVA) boost
Vaccine strain	A clade
Vaccine component	p17 Gag, p24 Gag

Notes

The HIV-1 subtype A focused vaccine HIVA contains p24 and p17, in a reversed order relative to the Gag polyprotein to prevent myristylation of p17, which could direct the protein to the cell membrane and inhibit efficient peptide processing and class I presentation, as well as a polyepitope string of conserved, often immunodominant epitopes that were selected to have particularly good cross-reactive potential for the A-clade epidemic in Nairobi, Kenya. A DNA and MVA prime-boost vaccination protocol using the HIVA antigen will be used in a phase III clinical trial in Kenya. This epitope is included in the polyepitope string Hanke2000.
Multiple CD4+ or CD8+ T-cell vaccine-induced responses to peptide pools were detected using intracellular cytokine staining and IFNgamma Elispot assays after vaccination of 5 macaques. The response to the Mamu A*01 SIV p27 epitope p11C (CTPYDINQM), included in the polyepitope region, was not immunodominant in the Mamu A*01 vaccinated macaques, possibly because of processing limitations in context of the artificial polyepitope string Wee2002.

References

Hanke2000 Tomas Hanke and Andrew J. McMichael. Design and construction of an experimental HIV-1 vaccine for a year-2000 clinical trial in Kenya. Nat. Med., 6(9):951-955, Sep 2000. PubMed ID: 10973301. Show all entries for this paper.

Wee2002 Edmund G.-T. Wee, Sandip Patel, Andrew J. McMichael, and Tomáš Hanke. A DNA/MVA-Based Candidate Human Immunodeficiency Virus Vaccine for Kenya Induces Multi-Specific T Cell Responses in Rhesus Macaques. J. Gen. Virol., 83(Pt 1):75-80, Jan 2002. PubMed ID: 11752703. Show all entries for this paper.

Displaying record number 52927

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag( B con)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Epitope Name	RL11
Subtype	B
Species (MHC/HLA)	human(B44)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity

Notes

CD8+ T-cell responses that persisted in individuals with advanced disease were studied; most of the optimal epitopes defined were recognized with intermediate to high avidity. On average 13 (range, 2-39) epitopic regions were targeted in an average of 6 proteins (range, 1-8). HAART resulted in decrease in antigen and reduction in gamma IFN EliSpot responses, suggesting active responses to autologous virus. The lack of mutations within most viral epitopes suggest that persistent CTL through late disease do not exert strong immune selection pressure, yet the Elispot assays show robust responses, suggesting to the authors that gamma IFN-based screening methods may not reveal functional CD8+ T-cell impairment in patients with AIDS.
1 subject recognized this epitope with low functional avidity. The autologous sequence matched the B consensus.

References

Draenert2004b R. Draenert, C. L. Verrill, Y. Tang, T. M. Allen, A. G. Wurcel, M. Boczanowski, A. Lechner, A. Y. Kim, T. Suscovich, N. V. Brown, M. M. Addo, and B. D. Walker. Persistent Recognition of Autologous Virus by High-Avidity CD8 T Cells in Chronic, Progressive Human Immunodeficiency Virus Type 1 Infection. J. Virol., 78(2):630-641, Jan 2004. PubMed ID: 14694094. Show all entries for this paper.

Displaying record number 315

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(293-312 LAI)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*44:02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a B*4402 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 60096

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	Gag(294-304)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
Epitope Name	RL11
Species (MHC/HLA)	human(C*03)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RDYVDRFYKTL had a significantly positive association with HLA C*03; it was recognized by 9/50 C*03-positive subjects and 9/291 C*03-negative subjects.

References

Displaying record number 62817

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24(294-304)
Epitope	`RDYVDRFYKTL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 77 single nucleotide mutations were observed in epitope RDYVDRFYKTL (known to bind to B4402) & variant RDYVDRFfKTL (known to bind to A2402) with a log transformation of mean fitness score, relative to NL4-3, of -0.7271.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.

Displaying record number 55936

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HXB2 Location	Gag(294-304) p24(162-172) DNA(1669..1701)	Gag Epitope Map
Author Location	p24
Epitope	`RDYVDRFYKTL`	Epitope Alignment
		Show epitope variants
Epitope Name	RL11(p24)
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity, chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Author defined epitope RDYVDRFYKTL elicited an immune response as part of peptide GPKEPFRDYVDRFYKTLR. In HLA-A24, -A26 and -B44 carriers, this epitope differs from the previously described epitope , RDYVDRFFKTL, at 1 residue, RDYVDRFyKTL.
5 of the 30 HLA-A24 carriers responded to RDYVDRFyKTL-containing peptide with average magnitude of CTL response of 182 SFC/million PBMC; 3 of the 8 HLA-A26 carriers responded with an average magnitude of CTL response of 333 SFC/million PBMC; 1 of the 6 HLA-B44 carriers responded with a magnitude of CTL response of 80 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.