Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 58058

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HXB2 Location	Nef(35-43) DNA(8899..8925)	Nef Epitope Map
Author Location	Nef
Epitope	RDLEKHGAI	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	A*02:01, A*32:01, B*37:01, B*39:06, C*06:02, C*07:02; A*02:01, A*26:01, B*37:01, B*38:01, C*06:02, C*12:03
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• T-cell epitope response specificities were examined in uninfected subjects who received the Gag/Pol/Nef vaccine used in the Step trial. Variable epitopes were detected more frequently than would be expected from an unbiased sampling. The most conserved epitopes were detected at a lower frequency, similar to the frequency reported for responses to natural infection, suggesting potential epitope masking. This may be a mechanism used by the virus in both contexts to escape effective T-cell surveillance. Development of vaccine strategies that can elicit a greater breadth, and towards conserved regions of the genome in particular, are critical requirements for effective T-cell based vaccines.
• All vacinees had responses to the Gag peptide pool, and most also had responses to the Nef and/or Pol pools. Subsequent mapping using 9mer minipools demonstrated that T-cell epitopes were recognized across the length of all 3 proteins. A total of 105 distinct 9mers were detected, although some of these may have been overlapping 9mers representing a single epitope.
• 2 vaccinees recognized 9mer RDLEKHGAI.

References

Li2011a Fusheng Li, Adam C. Finnefrock, Sheri A. Dubey, Bette T. M. Korber, James Szinger, Suzanne Cole, M. Juliana McElrath, John W. Shiver, Danilo R. Casimiro, Lawrence Corey, and Steven G Self. Mapping HIV-1 Vaccine Induced T-Cell Responses: Bias Towards Less-Conserved Regions and Potential Impact on Vaccine Efficacy in the Step Study. PLoS One, 6(6):e20479, 2011. PubMed ID: 21695251. Show all entries for this paper.

Displaying record number 59185

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HXB2 Location	Nef(35-43) DNA(8899..8925)	Nef Epitope Map
Author Location	Nef(35-43 NL432)
Epitope	RDLEKHGAI	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	AC01: A*01:01, A*31:01, B*37:01, B*51:09, C*01:02, C*06:02
Country	Australia, Germany, United States
Experimental methods	Other
Keywords	class I down-regulation by Nef, acute/early infection

Notes

• 10 acute controllers (AC) were compared to 50 acute progressors to analyze Nef function and downregulation of HLA Class I and CD4 levels. Nef-specific CTL of acute controllers downregulated HLA-I and CD4 less; with reduced virion infectivity. HLA-associated polymorphisms in Nef did effect impaired immune function in some controllers. In general however, controllers (6/10) had less Nef-induced viremic effects, due probably to host CTL pressure response.
• Baseline Nef 35-43 epitope sequence of patient AC01, RDLEKHGAI, is previously published and developed several polymorphisms with time. AC01 had a 19% reduction in HLA I downregulation and 10/11 nef clones isolated had 4 polymorphisms: R19K, H49R, M182L and V194A. It was studied in a B*37-expressing recipient.

References

Kuang2014 Xiaomei T. Kuang, Xiaoguang Li, Gursev Anmole, Philip Mwimanzi, Aniqa Shahid, Anh Q. Le, Louise Chong, Hua Qian, Toshiyuki Miura, Tristan Markle, Bemuluyigza Baraki, Elizabeth Connick, Eric S. Daar, Heiko Jessen, Anthony D. Kelleher, Susan Little, Martin Markowitz, Florencia Pereyra, Eric S. Rosenberg, Bruce D. Walker, Takamasa Ueno, Zabrina L. Brumme, and Mark A. Brockman. Impaired Nef Function Is Associated with Early Control of HIV-1 Viremia. J. Virol., 88(17):10200-10213, 1 Sep 2014. PubMed ID: 24965469. Show all entries for this paper.