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Displaying record number 55326

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HXB2 Location	Pol(361-369) RT(206-214) DNA(3165..3191)	Pol Epitope Map
Author Location	RT Pol
Epitope	`RAHLLSWGF`	Epitope Alignment
		Show epitope variants
Epitope Name	RT1
Subtype	A, B, C
Species (MHC/HLA)	mouse(H-2K^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	subtype comparisons, vaccine-induced immune responses, variant cross-reactivity, antagonism

Vaccine Details

Vaccine type	DNA with CMV promotor, modified vaccinia Ankara (MVA), DNA prime with modified vaccinia Ankara (MVA) boost
Vaccine strain	A clade, B clade, C clade Du422, other
Vaccine component	Gag, Nef, RT

Notes

Cross-clade vaccine recognition was studied using either single-, multi or several anatomically separated single-clade vaccines. Limited cross-clade response was seen with single-clade administrations. Multi-clade vaccines gave immune interference (antagonism and original antigenic sin), reducing CTL response. Simultaneously administered but anatomically separated vaccines from clades A, B, C decreased antagonism and increased immune responses.
After immunization with Clade A vaccine containing RT1, RAHLLSWGF, this index epitope was not recognized. Clade B vaccine containing RQHLLRWGL generated T cells that recognized its index epitope as well as variants RaHLLsWGf (Clade A index), RkHLLkWGf (Clade C index), RaHLLRWGf, RQHLLRWGf, ReHLLkWGf, ReHLLRWGf, RgHLLkWGf, RkHLLsWGf at lower levels. C Clade vaccine with index epitope RKHLLKWGF resulted in T cells that recognized that epitope, and Clade B index epitope RqHLLrWGl, and epitope RKHLLsWGF at 53%.

References

Larke2007 Natasha Larke, Eung-Jun Im, Ralf Wagner, Carolyn Williamson, Anna-Lise Williamson, Andrew J. McMichael, and Tomáš Hanke. Combined Single-Clade Candidate HIV-1 Vaccines Induce T Cell Responses Limited by Multiple Forms of In Vivo Immune Interference. Eur. J. Immunol., 37(2):566-577, Feb 2007. PubMed ID: 17230443. Show all entries for this paper.