Search CTL/CD8+ T-Cell Epitope Database

Found 20 matching records:

Displaying record number 60076

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*14; it was recognized by 7/32 B*14-positive subjects and 14/296 B*14-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*14 in a cohort of 245 controllers and 96 progressors.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 60077

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*18; it was recognized by 4/21 B*18-positive subjects and 7/265 B*18-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*18 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 60079

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B35:02, B35:03, B*53:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*3502,B*3503,B*5301; it was recognized by 2/27 B*3502,B*3503,B*5301-positive subjects and 0/188 B*3502,B*3503,B*5301-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*3502, -B*3503, -B*5301 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 60081

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*40; it was recognized by 2/21 B*40-positive subjects and 2/215 B*40-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*40 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 60078

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*45)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*45; it was recognized by 3/10 B*45-positive subjects and 11/286 B*45-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*45 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 60075

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*49)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA B*49; it was recognized by 8/13 B*49-positive subjects and 21/328 B*49-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-B*49 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 57635

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(173-181)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	p24 173-181
Subtype	B
Species (MHC/HLA)	human(B*51:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	dendritic cells, optimal epitope

Notes

The study showed for the first time that peptide-loaded, CD40L-matured dendritic cells from HI-1-infected persons on ART produced CD8 T cells for a much broader range and magnitude of Gag and Nef epitopes than did peptides without dendritic cells. Dendritic cells were used to reveal novel Gag and Nef epitopes.
Novel optimal B*5101-restricted epitope RAEQASQEV was mapped from a reactive 15mer.

References

Huang2010a Xiao-Li Huang, Zheng Fan, LuAnn Borowski, Robbie B. Mailliard, Morgane Rolland, James I. Mullins, Richard D. Day, and Charles R. Rinaldo. Dendritic Cells Reveal a Broad Range of MHC Class I Epitopes for HIV-1 in Persons with Suppressed Viral Load on Antiretroviral Therapy. PLoS One, 5(9):e12936, 2010. PubMed ID: 20886040. Show all entries for this paper.

Displaying record number 60080

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(C*05)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RAEQASQEV had a significantly positive association with HLA C*05; it was recognized by 3/29 C*05-positive subjects and 4/236 C*05-negative subjects.
RAEQASQEV was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-C*05 in a cohort of 245 controllers and 96 progressors.

References

Displaying record number 57286

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Subtype	B
Species (MHC/HLA)	human(C*08)
Immunogen	HIV-1 infection
Country	Barbados, Haiti, Jamaica
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	mother-to-infant transmission, elite controllers

Notes

To dissect whether the protective effect of HLA-B*57 in HIV-1 infection is due to CTL that recognize HLA-B*57-restricted epitopes or by the presence of viral-fitness attenuating mutations in such B*57 epitopes, 13 mother-infant pairs were studied. The B57 advantage is primarily through the maintenance of a fitness-attenuating mutation in p24-Gag, T242N in epitope TW10 (TSTLQEQIGW).
Regardless of parental inheritance, HLA-B57 controls VL even in infancy and even when escape mutations from B*57 positive mothers are transmitted. Mutations in p24-Gag are predominant in mismatched-HLA subject pairs and arise after vertical transmission, impacting viral fitness.
Non-B57-epitope RV9-p24, RAEQASQEV restricted by Cw*08, was recognized by an infant with maternally-inherited B57.

References

Schneidewind2009 Arne Schneidewind, Yanhua Tang, Mark A. Brockman, Elizabeth G. Ryland, Jacqueline Dunkley-Thompson, Julianne C. Steel-Duncan, M. Anne St. John, Joseph A. Conrad, Spyros A. Kalams, Francine Noel, Todd M. Allen, Celia D. Christie, and Margaret E. Feeney. Maternal Transmission of Human Immunodeficiency Virus Escape Mutations Subverts HLA-B57 Immunodominance but Facilitates Viral Control in the Haploidentical Infant. J. Virol., 83(17):8616-8627, Sep 2009. PubMed ID: 19515764. Show all entries for this paper.

Displaying record number 53247

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313 SUMA)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	Gag RV9
Subtype	B
Species (MHC/HLA)	human(C*08:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	SUMA: A11:03, A24:02, B14:02, B15:01, C08:02, C12:03
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	dynamics, acute/early infection, autologous responses, optimal epitope

Notes

Primary CD8+ T-cell response to Env, Tat and Gag and the extent, kinetics and mechanisms of viral escape were examined in three patients. Rapid escape, within weeks from infection, from HIV specific CTL responses was observed in all three patients, but the kinetics and extent of the escape differed depending on the breadth and co-dominant distribution of CTL-mediated pressure. The two patients that rapidly declined had more focused immunodominant responses, while the single patient that had low viral load and stable CD4 counts for seven years had a broad co-dominant response and less escape.
The patient SUMA maintained low viral loads and stable CD4 T cell counts through seven years of follow up. In contrast to more rapid progressors, WEAU and BORI, SUMA a broad response to 24 epitopes, with little immunodominance. Two peptides were somewhat more intensely recognized in acute infection, but this response leveled out early on.
Only four epitopes were found to acquire escape mutations in SUMA over time, and this was one of the 20 that remained invariant. A low level response was detected at acute infection that persisted through early infection.
Patient SUMA autologous epitopes DRFYKTLRAEQ & RAEQASQEV overlap.
Dynamics of this response are shown in Fig. S1 of Yang2019 which developed mathematical models with data from Jones2004. Yang2019 concluded that, in general, relative CTL response breadth increased moderately during the first symptomatic month and then remained relatively stable through the first 400 days post-infection. Analysis also suggested slow expansion kinetics for most HIV-specific CTL responses and the presence of intra- and interclonal competition.

References

Jones2004 Nicola A. Jones, Xiping Wei, Darren R. Flower, MaiLee Wong, Franziska Michor, Michael S. Saag, Beatrice H. Hahn, Martin A. Nowak, George M. Shaw, and Persephone Borrow. Determinants of Human Immunodeficiency Virus Type 1 Escape from the Primary CD8+ Cytotoxic T Lymphocyte Response. J. Exp. Med., 200(10):1243-1256, 15 Nov 2004. PubMed ID: 15545352. Show all entries for this paper.

Yang2019 Yiding Yang, and Vitaly V. Ganusov. Defining Kinetic Properties of HIV-Specific CD8(+) T-Cell Responses in Acute Infection. Microorganisms, 7(3):microorganisms7030069 doi, Mar 2019. PubMed ID: 30836625 Show all entries for this paper.

Displaying record number 332

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Originally reported as HLA-B14 restricted, but subsequently found not to be presented by cells transfected with B14.
Thought to be HLA-Cw8 restricted (C. Brander and B. Walker)

References

Johnson1991 R. P. Johnson, A. Trocha, L. Yang, G. P. Mazzara, D. L. Panicali, T. M. Buchanan, and B. D. Walker. HIV-1 Gag-Specific Cytotoxic T Lymphocytes Recognize Multiple Highly Conserved Epitopes. Fine Specificity of the Gag-Specific Response Defined by Using Unstimulated Peripheral Blood Mononuclear Cells and Cloned Effector Cells. J. Immunol., 147:1512-1521, 1991. This study presented a detailed study of gag-specific CTL from HIV-1 seropositive individuals. Seven p24 and two p17 epitopes were described, that were recognized by class I-restricted CD3+CD8+ CTL. p17 epitopes: KIRLRPGGKKKYKLKHIVWASRELE and QTGSEELRSLYNTVATLYCVHQRIE; p24 epitopes: NPPIPVGEIYKRWIILGLNKIV, VHQAISPRTLNAWVKVVEEKAF, NAWVKVVEEKAFSPEVIPMFSA, SALSEGATPQDLNTMLNTVGGH, GHQAAMQMLKETINEEAAEWDR, and RAEQASQEVK. PubMed ID: 1715361. Show all entries for this paper.

Displaying record number 333

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24
Epitope	`RAEQASQEV`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 exposed seronegative
Experimental methods
Keywords	subtype comparisons, HIV exposed persistently seronegative (HEPS)

Notes

A CTL response was found in exposed but uninfected prostitutes from Nairobi using previously-defined B clade epitopes that tended to be conserved in A and D clades -- such cross-reactivity could protect against both A and D and confer protection in Nairobi where both subtypes are circulating.
The A subtype consensus is RAeQAtQEV.
The D subtype consensus is RAEQsQdV.
Thought to be HLA-Cw8 restricted, not B14 as originally reported (C. Brander, B. Walker, and S. Rowland-Jones, personal communication)
Database note: First author "RowlandJones" is also found as "Rowland-Jones" on annotated papers in this database.

References

RowlandJones1998b S. Rowland-Jones, T. Dong, P. Krausa, J. Sutton, H. Newell, K. Ariyoshi, F. Gotch, S. Sabally, T. Corrah, J. Kimani, K. MacDonald, F. Plummer, J. Ndinya-Achola, H. Whittle, and A. McMichael. The Role of Cytotoxic T Cells in HIV Infection. Dev. Biol. Stand., 92:209-214, 1998. In this paper CTL response to previously defined conserved epitopes was found in exposed but uninfected prostitutes in Nairobi. Subtypes A and D are circulating in this regions, and the reactive epitopes tended to be conserved. Similarly previous studies in the Gambia showed that exposed but uninfected prostitutes tended to have B35 presented CTL epitopes conserved between HIV-1 and HIV-2. It was suggested that what was special about B35 is simply that it presents epitopes found both in HIV-1 and HIV-2. PubMed ID: 9554277. Show all entries for this paper.

Displaying record number 334

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Study of cytokines released by HIV-1 specific activated CTL.
Thought to be HLA-Cw8 restricted, not B14 as originally reported (C. Brander, B. Walker, and S. Rowland-Jones, personal communication)

References

Price1995 P. Price, R. P. Johnson, D. T. Scadden, C. Jassoy, T. Rosenthal, S. Kalams, and B. D. Walker. Cytotoxic CD8+ T Lymphocytes Reactive with Human Immunodeficiency Virus-1 Produce Granulocyte/Macrophage Colony-Stimulating Factor and Variable Amounts of Interleukins 2, 3, and 4 following Stimulation with the Cognate Epitope. Clin. Immunol. Immunopathol., 74:100-106, 1995. Cytokine release from stimulated CTL clones derived from either the peripheral blood or CSF of 3 patients was studied. HLA restriction was determined for two of seven clones. GM-CSF and TNF-$\alpha$ and IFN-$\gamma$ were produced by all clones; most clones produced low amounts of IL-2, IL-3, and IL-4. PubMed ID: 7527747. Show all entries for this paper.

Displaying record number 335

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Eighty two HIV-1-specific CTL clones from 5 long-term non-progressors were isolated and analyzed for breadth of response.
A sustained Gag, Env and Nef response was observed, and clones were restricted by multiple HLA epitopes, indicating a polyclonal response.
Despite this being a well-defined conserved epitope, and thought to be presented by B14, none of the 11 gag-specific clones from a B-14 positive subject could recognize either it or p24 PQDLNTMLN.
Thought to be HLA-Cw8 restricted, not B14 as originally reported (C. Brander, B. Walker, and S. Rowland-Jones, personal communication)

References

Lubaki1997 N. M. Lubaki, S. C. Ray, B. Dhruva, T. C. Quinn, R. F. Siliciano, and R. C. Bollinger. Characterization of a Polyclonal Cytolytic T Lymphocyte Response to Human Immunodeficiency Virus in Persons without Clinical Progression. J. Infect. Dis., 6:1360-1367, 1997. Five individuals were studied who survived HIV infection in good health for over 5 years. A broad polyclonal response was found to multiple proteins. PubMed ID: 9180175. Show all entries for this paper.

Displaying record number 1678

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 53756

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag(305-313)
Epitope	`RAEQASQEV`	Epitope Alignment
		Show epitope variants
Epitope Name	RV9
Subtype	B
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A02, A68, B14, B52, C08, C12
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay
Keywords	escape, optimal epitope

Notes

HIV-1-specific CD8 T-cell responses were persistent in the chronic phase of HIV-1 infection, although the responses to some of the epitopes declined despite the persistence of the targeted sequences in vivo. Only 4/14 epitopes were potential CTL escape variants, although strong responses to these epitopes persisted for 6 years. This indicates limited viral evolution within targeted CD8 T-cell epitopes during the chronic phase of infection.
Elispot responses to the consensus form of this epitope, RAEQASQEV, were much more intense than to the most common variants of the epitope found over time in this individual, RAEQAStEV and RAEQASQdV. There was a diminished response to RAEQAStEV and no response to RAEQASQdV, so these appear to be escape variants. The strong response to the consensus form persisted, despite the fact it was not observed among the autologous sequences until it surfaced as a minor variant (5/13 sequences) after 6 years of chronic infection.

References

Koibuchi2005a Tomohiko Koibuchi, Todd M. Allen, Mathias Lichterfeld, Stanley K. Mui, Kristin M. O'Sullivan, Alicja Trocha, Spyros A. Kalams, R. Paul Johnson, and Bruce D. Walker. Limited Sequence Evolution within Persistently Targeted CD8 Epitopes in Chronic Human Immunodeficiency Virus Type 1 Infection. J. Virol., 79(13):8171-8181, Jul 2005. PubMed ID: 15956562. Show all entries for this paper.

Displaying record number 54276

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	p24
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	Cw8-RV9(p24)
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contributions of immune responses restricted to individual HLA class I molecules were studied.
This epitope-HLA combination was recognized. The most frequently recognized epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contributions of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 55050

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope RAEQASQEV elicited a magnitude of response of 220 SFC with a functional avidity of 0.01nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 1328

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location
Epitope	`RAEQASQEV`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

This study examines CTL responses in HIV exposed, persistently seronegative individuals, HEPS, who eventually seroconverted -- 11/114 HEPS Nairobi sex workers eventually seroconverted, and for six of these HIV CTL reactive epitopes had been defined while seronegative.
The epidemiological factor associated with seroconversion was stopping sex work. HIV-specific CTL activity declined when HEPS sex workers stopped working for a period or retired.
This epitope was recognized in 1/22 HEPS sex worker controls ML1792.

References

Kaul2001 R. Kaul, S. L. Rowland-Jones, J. Kimani, T. Dong, H. B. Yang, P. Kiama, T. Rostron, E. Njagi, J. J. Bwayo, K. S. MacDonald, A. J. McMichael, and F. A. Plummer. Late Seroconversion in HIV-Resistant Nairobi Prostitutes Despite Pre-Existing HIV-Specific CD8+ Responses. J. Clin. Invest., 107(3):341-349, Feb 2001. URL: http://www.jci.org/cgi/content/full/107/3/341. PubMed ID: 11160158. Show all entries for this paper.

Displaying record number 61472

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HXB2 Location	Gag(305-313) p24(173-181) DNA(1702..1728)	Gag Epitope Map
Author Location	Gag( 90TH_BK132, US1_GS004/7, J3222, JR-CSF, NL4-3, LAI)
Epitope	`RAEQASQEV`	Epitope Alignment
Epitope Name	RV9
Subtype	B
Species (MHC/HLA)	human
Immunogen	in vitro stimulation or selection, HIV-1 infection, peptide-HLA interaction
Country	Canada, United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	assay or method development, memory cells

Notes

Using CTL clones as biosensors multiple LRA (latency reversing agents) were tested for efficacy in sensitizing latently-infected cells for recognition by HIV-epitope-specific CTLs. This novel experimental approach for evaluation of LRAs and latency reversal as well as amplified detection of CTL shock-and-kill eradication strategies identified several LRAs, viz. IL-15SA, IL-2, IL-15, prostratin, and TLR-2 ligand Pam₃CSK₄. IL-15 superagonist, ALT-803, primed the natural ex vivo resting, latent CD4+ T-cell-reservoir for CD8+ T cell recognition, at clinically achievable concentrations.
An HIV-Gag-RV9 (RAEQASQEV) specific T-cell clone from subject OM292 was able to recognize LRA-primed HIV-latently-infected CD4+ T cells.

References

Jones2016 R. Brad Jones, Stefanie Mueller, Rachel O'Connor, Katherine Rimpel, Derek D. Sloan, Dan Karel, Hing C. Wong, Emily K. Jeng, Allison S. Thomas, James B. Whitney, So-Yon Lim, Colin Kovacs, Erika Benko, Sara Karandish, Szu-Han Huang, Maria J. Buzon, Mathias Lichterfeld, Alivelu Irrinki, Jeffrey P. Murry, Angela Tsai, Helen Yu, Romas Geleziunas, Alicja Trocha, Mario A. Ostrowski, Darrell J. Irvine, and Bruce D. Walker. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes. PLoS Pathog., 12(4):e1005545, Apr 2016. PubMed ID: 27082643. Show all entries for this paper.