Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 58405

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef
Epitope	QVPLRPMTY	Epitope Alignment
Epitope Name	Nef073
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(A1)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire, Denmark, Ghana, Guinea-Bissau, Mali, Senegal
Experimental methods	Intracellular cytokine staining, Pentamer labeling
Keywords	vaccine antigen design

Notes

• 15 subdominant CTL epitopes were used to study 2 new Guinea-Bissau HIV-1 isolates, a newly completed Danish subtype B genome sequence and other previously published West African sequences. Subtype analysis showed the 2 new Guinea-Bissau sequences were CRF02_AG inter-subtype recombinant forms with typical cross-over points. The 15 epitopes were restricted by common HLA alleles (A1, A2, A3, B7, B44) and were present and conserved in all sequences studied. Mean conservation of the epitopes in local strains was 76% and 70% for Denmark and West Africa respectively. All epitopes had been previously confirmed as antigenic [Thorn2007, Perez2008].
• Nef073 epitope, QVPLRPMTY, showed a conservation of amino acid identity of 83% for the Danish sequence and 65% for the West African sequences.

References

Perez2008 Carina L. Pérez, Mette V. Larsen, Rasmus Gustafsson, Melissa M. Norström, Ann Atlas, Douglas F. Nixon, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Broadly Immunogenic HLA Class I Supertype-Restricted Elite CTL Epitopes Recognized in a Diverse Population Infected with Different HIV-1 Subtypes. J. Immunol., 180(7):5092-5100, 1 Apr 2008. PubMed ID: 18354235. Show all entries for this paper.

Thorn2007 Mette Thorn, Sheila Tang, Dominic Therrien, Henrik Kløverpris, Lasse Vinner, Gitte Kronborg, Jan Gerstoft, Sylvie Corbet, and Anders Fomsgaard. Sequence Conservation of Subdominant HLA-A2-Binding CTL Epitopes in HIV-1 Clinical Isolates and CD8+ T-Lymphocyte Cross-Recognition May Explain the Immune Reaction in Infected Individuals. APMIS, 115(6):757-768, Jun 2007. PubMed ID: 17550385. Show all entries for this paper.

Vinner2011 Lasse Vinner, Birgitta Holmgren, Kristoffer J. Jensen, Joakim Esbjornsson, Marie Borggren, Julie L. Hentze, Ingrid Karlsson, Betina S. Andresen, Gregers J. Gram, Henrik Kloverpris, Peter Aaby, Zacarias José Da Silva, Eva-Maria Fenyö, and Anders Fomsgaard. Sequence Analysis of HIV-1 Isolates from Guinea-Bissau: Selection of Vaccine Epitopes Relevant in Both West African and European Countries. APMIS, 119(8):487-497, Aug 2011. PubMed ID: 21749448. Show all entries for this paper.

Displaying record number 61956

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef(73-)
Epitope	QVPLRPMTY	Epitope Alignment
Epitope Name	QY9
Subtype	A3, B, C, CRF02_AG
Species (MHC/HLA)	human(A*80:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	1213: A*30:01, A*30:02, B*07:02, B*58:01
Country	Denmark, Guinea-Bissau
Experimental methods	HLA binding, Intracellular cytokine staining, Tetramer binding
Keywords	binding affinity, vaccine antigen design

Notes

• 95% and 100% of untreated, asymptomatic HIV-1-infected patients, from Guinea-Bissau and Denmark respectively, were able to subdominantly recognize 15 predicted CTL epitopes restricted by at least one of the 5 most common HLA supertypes, HLA-A*01, -A*02, -A*03, -B*07 and -B*44.
• Epitope QY9, QVPLRPMTY, predicted and validated to be restricted by supertype B07, was found across geographic regions in 83% of HIV-1 sequences in Denmark clade-B and 65% Guinea-Bissau/Western Africa subtypes A3, C and CRF02_AG. Its equivalent peptide, Nef73 was able to elicit cytokine production and bound the following HLA alleles moderately or well: A*8001 (B07 supertype).

References

Karlsson2012 Ingrid Karlsson, Henrik Kløverpris, Kristoffer Jarlov Jensen, Anette Stryhn, Søren Buus, Annika Karlsson, Lasse Vinner, Philip Goulder, and Anders Fomsgaard. Identification of Conserved Subdominant HIV Type 1 CD8+ T Cell Epitopes Restricted within Common HLA Supertypes for Therapeutic HIV Type 1 Vaccines. AIDS Res. Hum. Retroviruses, 28(11):1434-1443, Nov 2012. PubMed ID: 22747336. Show all entries for this paper.

Displaying record number 59004

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef(73-)
Epitope	QVPLRPMTY	Epitope Alignment
Epitope Name	Nef73
Subtype	A
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	P2: A*68:02, A*74:01, B*35:01, B*53:01; P15: A*23:01, A*31:01, B*35:01, B*41:02; P21: A*02:02, A*23:01, B*14:01, B*35:01
Country	Guinea-Bissau
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, vaccine antigen design, therapeutic vaccine, chronic infection

Vaccine Details

Notes

• Therapeutic vaccination of chronically infected HIV-1 subjects was evaluated as a means of halting disease progression. A subdominant peptide vaccine containing 15 CTL epitopes and 3 helper T-cell peptides along with an adjuvant was delivered to 18 infected subjects, and this Guinea-Bissau study compared to a similar Danish one. Using IFN-γ EliSpot, 9 new T-cell responses were detected in 6/14 subjects whose viral loads (VL) remained stable. Other pre-existing responses, including CTL responses, however, were not significantly detected.
• Nef73, QVPLRPMTY, is a CTL epitope sequence used as a vaccine component.

References

Roman2013 Victor Raúl Gómez Román, Kristoffer Jarlov Jensen, Sanne Skov Jensen, Christian Leo-Hansen, Sanne Jespersen, David da Silva Té, Candida Medina Rodrigues, Christoph Mikkel Janitzek, Lasse Vinner, Terese Lea Katzenstein, Peter Andersen, Ingrid Kromann, Lars Vibe Andreasen, Ingrid Karlsson, and Anders Fomsgaard. Therapeutic Vaccination Using Cationic Liposome-Adjuvanted HIV Type 1 Peptides Representing HLA-Supertype-Restricted Subdominant T Cell Epitopes: Safety, Immunogenicity, and Feasibility in Guinea-Bissau. AIDS Res Hum Retroviruses, 29(11):1504-1512, Nov 2013. PubMed ID: 23634822. Show all entries for this paper.

Displaying record number 59126

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef(73-81 NL4.3)
Epitope	QVPLRPMTY	Epitope Alignment
Epitope Name	QY9
Subtype	B
Species (MHC/HLA)	human(B*35)
Immunogen	HIV-1 infected monocyte-derived
Country	United States
Experimental methods	Chromium-release assay, Other
Keywords	epitope processing, dendritic cells

Notes

• Variations in HIV-1 peptide degradation pattern, kinetics, as well as production and degradation of HIV epitopes were correlated with cytosolic antigen-processing machinery of either monocyte-derived dendritic cells (DCs) or macrophages (MPs) after maturation with TRL4, TLR7/8 ligands. Generation of epitope precursors and epitopes from epitope-containing peptides was dependent on specific epitope and cytosolic extract type, leading to a 2- to 25-fold difference in CTL response in DCs, MPs and monocytes.
• A synthetic 28-mer, AQEEEEVGFPVTPQVPLRPMTYKAAVDL, 13-QY9-6, containing a B*35-restricted epitope QVPLRPMTY, QY9, produced more QY9 faster, along with N- and C- terminal extended versions as well as antitopes, when incubated with monocyte- rather than DC or MP-cytosolic extracts, though all were efficient.

References

Dinter2014 Jens Dinter, Pauline Gourdain, Nicole Y. Lai, Ellen Duong, Edith Bracho-Sanchez, Marijana Rucevic, Paul H. Liebesny, Yang Xu, Mariko Shimada, Musie Ghebremichael, Daniel G. Kavanagh, and Sylvie Le Gall. Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition. J. Immunol., 193(9):4322-4334, 1 Nov 2014. PubMed ID: 25230751. Show all entries for this paper.

Displaying record number 56481

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef
Epitope	QVPLRPMTY	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*35:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	viral fitness and reversion, HLA associated polymorphism

Notes

• HLA-associated polymorphisms were studied in a cohort of 710 chronic subtype C patients from South Africa. 84 HLA Class I-associated polymorphisms were identified, 73% of them with HLA-B. The viral set points associated with HLA allele correlated with the number of reversions associated with that allele, (HLA-B in Gag, HLA-C in Pol).
• QVPLRPMTY is a previously described HLA-B*3501-restricted epitope (part of Nef reacting peptide RPQVPLRPMTyKAAFDLSFFL) that contains a B*3501-associated reversion at residue Y (QVPLRPMTy).

References

Matthews2008 Philippa C. Matthews, Andrew Prendergast, Alasdair Leslie, Hayley Crawford, Rebecca Payne, Christine Rousseau, Morgane Rolland, Isobella Honeyborne, Jonathan Carlson, Carl Kadie, Christian Brander, Karen Bishop, Nonkululeko Mlotshwa, James I. Mullins, Hoosen Coovadia, Thumbi Ndung'u, Bruce D. Walker, David Heckerman, and Philip J. R. Goulder. Central Role of Reverting Mutations in HLA Associations with Human Immunodeficiency Virus Set Point. J. Virol., 82(17):8548-8559, Sep 2008. PubMed ID: 18596105. Show all entries for this paper.

Displaying record number 57494

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HXB2 Location	Nef(73-81) DNA(9013..9039)	Nef Epitope Map
Author Location	Nef(73-81)
Epitope	QVPLRPMTY	Epitope Alignment
		Show epitope variants
Epitope Name	QY9
Subtype	B, C, D
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Finland, Sweden, Uganda
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity

Notes

• 6 epitopes and their variants were tested for quantitative CTL recognition in a genetically diverse cohort of 26 HIV-1+ subjects from 12 countries, to determine response pattern as correlating to HLA genotype & autologous virus sequence. Cross-reactive epitopes were more similar, with a single substitution decreasing recognition by 40%. Central residues in an epitope were more important for recognition.
• Epitope QY9(73-81), QVPLRPMTY, elicited CTL response when tested in 3 patients - 2295 (Caucasian female, experienced some treatment, autologous virus Clade C); 2792 (Caucasian male, treatment naive, autologous virus Clade B); 2840 (African female, treated, autologous virus Clade D).

References

Hoof2010 Ilka Hoof, Carina L. Pérez, Marcus Buggert, Rasmus K. L. Gustafsson, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Interdisciplinary Analysis of HIV-Specific CD8+ T Cell Responses against Variant Epitopes Reveals Restricted TCR Promiscuity. J Immunol, 184(9):5383-5391, 1 May 2010. PubMed ID: 20363973. Show all entries for this paper.