Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 56634

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HXB2 Location	Rev(47-55) DNA(8441..8467)	Rev Epitope Map
Author Location	Rev(47-56)
Epitope	QRQRQIQSL	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	escape, acute/early infection

Notes

• Using overlapping 15-mer peptides, CTL responses to early, acute HIV infection were determined. Early virus escape attributed to CTL response was charted and subsequent clusters of mutations due to NAb targeting were also found.
• Five findings made: (i) exact numbers of transmitted HIV-1 actual and potential T cell epitopes were determined (ii) CTL-mediated virus escape occurring as viremia peaks, drops and reaches set point earlier and faster than previously known (iii) CTL responses to some transmitted virus epitopes were lost after early escapes were selected (iv) a few unexplained escapes were probably due to bystander mutations (v) earliest CTL responses cause the decline of VL in acute infection.
• This epitope, QRQRQIQSL, was studied in patient SUMA0874. 20 days following seroconversion only 1 variant was detected, but by D34SFOx 22/28 sequences contained such nonsynonymous substitutions.
• Variants of QRQRQIQSL seen in Patient SUMA0874 were QkQRQIQSL, QRQRhIQSL, QRQRQIrSL, QRQRQIQlL and QRQRQIQSi.

References

Goonetilleke2009 Nilu Goonetilleke, Michael K. P. Liu, Jesus F. Salazar-Gonzalez, Guido Ferrari, Elena Giorgi, Vitaly V. Ganusov, Brandon F. Keele, Gerald H. Learn, Emma L. Turnbull, Maria G Salazar, Kent J Weinhold, Stephen Moore, CHAVI Clinical Core B, Norman Letvin, Barton F. Haynes, Myron S. Cohen, Peter Hraber, Tanmoy Bhattacharya, Persephone Borrow, Alan S. Perelson, Beatrice H. Hahn, George M. Shaw, Bette T. Korber, and Andrew J. McMichael. The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection. J. Exp. Med., 206(6):1253-1272, 8 Jun 2009. PubMed ID: 19487423. Show all entries for this paper.

Displaying record number 57433

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HXB2 Location	Rev(47-55) DNA(8441..8467)	Rev Epitope Map
Author Location	Rev
Epitope	QRQRQIQSL	Epitope Alignment
		Show epitope variants
Epitope Name	QL9
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, acute/early infection

Notes

• 3 subjects with acute HIV infection were studied longitudinally by 454-ultra deep-sequencing of 4 HIV-1 epitope regions that have early recognition and escape. Over 50 variant forms of each CTL epitope targeted during the first 3-6 weeks of infection were found, i.e. 7-25 times more than variation in the control V3 region of Env, at earlier times. In very early infection, reversions were seen where immune pressure was considerable but they were absent outside epitopes. A strong case was made for establishment of infection via a single virus.
• All 3 subjects' epitopes have been tested in previous publications.
• Epitope QRQRQIQSLS is the transmitted form of Rev QL9 as determined by ELISpot on days 4, 5, 13, 20, 34 postinfection. It has an accumulation/loss rate of -0.42, diminishing to 4.95% of all QL9 variants by day 41. The 3 escape lineages that come from QL9 are: QRQRQIQSi; QRQRQIrSL; QRQRQIkSL in patient SUMA. Immune selection is estimated to have begun around day 18.

References

Fischer2010 Will Fischer, Vitaly V. Ganusov, Elena E. Giorgi, Peter T. Hraber, Brandon F. Keele, Thomas Leitner, Cliff S. Han, Cheryl D. Gleasner, Lance Green, Chien-Chi Lo, Ambarish Nag, Timothy C. Wallstrom, Shuyi Wang, Andrew J. McMichael, Barton F. Haynes, Beatrice H. Hahn, Alan S. Perelson, Persephone Borrow, George M. Shaw, Tanmoy Bhattacharya, and Bette T. Korber. Transmission of Single HIV-1 Genomes and Dynamics of Early Immune Escape Revealed by Ultra-Deep Sequencing. PLoS One, 5(8):e12303, 2010. PubMed ID: 20808830. Show all entries for this paper.