Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 55379

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HXB2 Location	gp160(67-75) gp120(67-75) DNA(6423..6449)	gp160 Epitope Map
Author Location
Epitope	NVWATHACV	Epitope Alignment
		Show epitope variants
Epitope Name	Env 67(2V)
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Denmark
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	variant cross-reactivity

Notes

• 11 HLA-A2+ (A*0201, A*0220, A*0234 or A*0236), patients' HIV were sequenced. Of 28 epitopes studied, most were conserved and elicited a subdominant CTL response. 9 epitopes in 7 proteins showed T-cell cross reaction. These epitopes were optimized to induce more immunogenic but cross-reacting responses.
• Epitopes in Gag, Pol, Env, Nef were more conserved than epitopes in shorter proteins Vpu, Vif, Vpr and Rev. Most natural epitopes were untargeted or targeted by 1 or 2 patients.
• Previously described Env 67(2V) epitope, NVWATHACV, was found in 10 patients but none had CTL immune responses to it. Response to the Env 67(2I)var, NiWATHACV, was however detected in one case.
• Env 67 is one of the most conserved epitopes in Env. Its variant Env 67(2V) was less targeted and immunogenic. Rare variant Env 67(2I) was more immunogenic and was also recognized by 1 in 16 HLA-A2- patients.

References

Thorn2007 Mette Thorn, Sheila Tang, Dominic Therrien, Henrik Kløverpris, Lasse Vinner, Gitte Kronborg, Jan Gerstoft, Sylvie Corbet, and Anders Fomsgaard. Sequence Conservation of Subdominant HLA-A2-Binding CTL Epitopes in HIV-1 Clinical Isolates and CD8+ T-Lymphocyte Cross-Recognition May Explain the Immune Reaction in Infected Individuals. APMIS, 115(6):757-768, Jun 2007. PubMed ID: 17550385. Show all entries for this paper.

Displaying record number 61950

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HXB2 Location	gp160(67-75) gp120(67-75) DNA(6423..6449)	gp160 Epitope Map
Author Location	Env(67-)
Epitope	NVWATHACV	Epitope Alignment
Epitope Name	NV9
Subtype	A3, B, C, CRF02_AG
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Denmark, Guinea-Bissau
Experimental methods	HLA binding, Intracellular cytokine staining, Tetramer binding
Keywords	binding affinity, vaccine antigen design, non-susceptible form

Notes

• 95% and 100% of untreated, asymptomatic HIV-1-infected patients, from Guinea-Bissau and Denmark respectively, were able to subdominantly recognize 15 predicted CTL epitopes restricted by at least one of the 5 most common HLA supertypes, HLA-A*01, -A*02, -A*03, -B*07 and -B*44.
• Epitope NV9, NVWATHACV, predicted and validated to be restricted by supertype A02, was found across geographic regions in 94% of HIV-1 sequences in Denmark clade-B and 100% Guinea-Bissau/Western Africa subtypes A3, C and CRF02_AG. Its equivalent peptide, Env67 of the Vif/Vpu A02 pool did not elicit cytokine production.

References

Karlsson2012 Ingrid Karlsson, Henrik Kløverpris, Kristoffer Jarlov Jensen, Anette Stryhn, Søren Buus, Annika Karlsson, Lasse Vinner, Philip Goulder, and Anders Fomsgaard. Identification of Conserved Subdominant HIV Type 1 CD8+ T Cell Epitopes Restricted within Common HLA Supertypes for Therapeutic HIV Type 1 Vaccines. AIDS Res. Hum. Retroviruses, 28(11):1434-1443, Nov 2012. PubMed ID: 22747336. Show all entries for this paper.