Search CTL/CD8+ T-Cell Epitope Database

Found 10 matching records:

Displaying record number 53934

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	( C consensus)
Epitope	`NPEIVIYQY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
Mutational patterns in the I4 residue of NPEIVIYQY are associated with the presence of the HLA presenting molecule in the host.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 60031

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
NPEIVIYQY had a significantly positive association with HLA B*18; it was recognized by 6/20 B*18-positive subjects and 15/284 B*18-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 54100

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(B*18)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a B*18 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 54003

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	( C consensus)
Epitope	`NPEIVIYQY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*35)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
NPEIVIYQY is an optimal epitope.

References

Displaying record number 61555

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	RT(175-183)
Epitope	`NPEIVIYQY`	Epitope Alignment
		Show epitope variants
Epitope Name	NY9
Subtype	CRF01_AE
Species (MHC/HLA)	human(B*35:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	KI-667: ; KI-805: ; KI-1124:
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining
Keywords	subtype comparisons, variant cross-reactivity, mutation acquisition, drug resistance

Notes

Nonnucleoside Reverse Transcriptase Inhibitor (NNRTI)-resistance mutations at Y181 in RT are known to overlap with 3 epitopes (IV10, IYQYMDDLYV; NY9, NPDIVIYQY or NPEIVIYQY; and KY9, KQNPDIVIY) in HIVs-B and -A/E. The effect of 3 different mutations, Y181I/V/C on recognition of the 3 epitopes across Subtype B and A/E viruses and in Japanese and Vietnamese populations was studied. Different effects of NNRTI-resistance RT181 mutations on CTL responses between the 2 subtype infections were demonstrated, particulary for NY9 .
The NY9-3E subtype A/E epitope, NPEIVIYQY, elicited a response from NY9-specific CTL (Japanese subjects KI-667, KI-805, KI-1124) in 3/5 patients infected with A/E. NY9-3E-specific CTL cross-recognized all three of its mutant peptides - NY9-3E7I, NY9-3E7V, and NY9-3E7C.
Y181C mutation was not seen in Japanese treatment-naive subjects but was seen to accumulate in subtype A/E-infected Vietnamese NNRTI-naive subjects.
Effects of Y181 mutations for B*3501-restricted NY9-3E-specific bulk T cells from 2 subjects were unique as compared to subtype B NY9-specific CTL clones. Such cells from KI-667 recognized WT NY9-3E, NY9-3E7I but only weakly recognized NY9-3E7C and NY9-3E7V. Cells from KI-805, however, recognized WT and NY9-3E7C similarly, but NY9-3E7V and NY9-3E7I were more strongly recognized. Mutations were thought to partially affect epitope recognition by TCR by CTL from KI-667 but not KI-805.

References

Kuse2015 Nozomi Kuse, Mohammad Arif Rahman, Hayato Murakoshi, Giang Van Tran, Takayuki Chikata, Madoka Koyanagi, Kinh Van Nguyen, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Different Effects of Nonnucleoside Reverse Transcriptase Inhibitor Resistance Mutations on Cytotoxic T Lymphocyte Recognition between HIV-1 Subtype B and Subtype A/E Infections. J. Virol., 89(14):7363-7372, Jul 2015. PubMed ID: 25972553. Show all entries for this paper.

Displaying record number 58659

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	RT NY9
Subtype	C
Species (MHC/HLA)	human(B*35:01)
Immunogen	HIV-1 infection
Country	Barbados, Botswana, Haiti, Japan, Mexico, South Africa, United Kingdom, United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons

Notes

While host HLA Class I genotype is the major genetic determinant of HIV-1 progression to AIDS, the same HLA allele could have varying outcomes for different infecting HIV clades. Overall, p24 Gag-specific epitopes were targeted significantly more frequently by C-clade-infected B*3501-positive subjects (55%) than B-clade-carrying B*3501-subjects (31%), while Env-specific epitopes were targeted more frequently by B-clade-infected subjects.
In general HLA-B*3501-restricted epitopes, were not targeted differentially for different clades. In contrast, CTL targeting against B*3501-restricted clade C Gag epitope, NPPIPVGDIY (NY10) but not clade B NY10, was associated with a lower viral set point and higher absolute CD4 count, i.e. better disease control. The above major result for epitope NY10 was confirmed for the B clade variant in Caucasian, Japanese and Mexican cohorts; C clade results were confirmed in South African (Durban) and Botswanan (Gaborne) cohorts.
In addition 5 new C-clade epitopes were identified.
C-clade specific epitope, RT NY9, NPEIVIYQY, elicited CTL response in >30% of C-clade infected subjects. The B-clade form of this epitope, NPdIVIYQY, was tested with cells from B-clade-infected subjects who responded to it.

References

Matthews2012 Philippa C. Matthews, Madoka Koyanagi, Henrik N. Kløverpris, Mikkel Harndahl, Anette Stryhn, Tomohiro Akahoshi, Hiroyuki Gatanaga, Shinichi Oka, Claudia Juarez Molina, Humberto Valenzuela Ponce, Santiago Avila Rios, David Cole, Jonathan Carlson, Rebecca P. Payne, Anthony Ogwu, Alfred Bere, Thumbi Ndung'u, Kamini Gounder, Fabian Chen, Lynn Riddell, Graz Luzzi, Roger Shapiro, Christian Brander, Bruce Walker, Andrew K. Sewell, Gustavo Reyes Teran, David Heckerman, Eric Hunter, Søren Buus, Masafumi Takiguchi, and Philip J. R. Goulder. Differential Clade-Specific HLA-B*3501 Association with HIV-1 Disease Outcome Is Linked to Immunogenicity of a Single Gag Epitope. J. Virol., 86(23):12643-12654, Dec 2012. PubMed ID: 22973023. Show all entries for this paper.

Displaying record number 60030

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(B35:01, B35:08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
NPEIVIYQY had a significantly positive association with HLA B*3501,B*3508; it was recognized by 7/35 B*3501,B*3508-positive subjects and 8/249 B*3501,B*3508-negative subjects.

References

Displaying record number 60029

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(B35:02, B35:03, B*53:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
NPEIVIYQY had a significantly positive association with HLA B*3502,B*3503,B*5301; it was recognized by 19/37 B*3502,B*3503,B*5301-positive subjects and 21/304 B*3502,B*3503,B*5301-negative subjects.

References

Displaying record number 60033

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
NPEIVIYQY had a significantly positive association with HLA B*4201; it was recognized by 1/4 B*4201-positive subjects and 0/151 B*4201-negative subjects.

References

Displaying record number 60032

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HXB2 Location	Pol(330-338) RT(175-183) DNA(3072..3098)	Pol Epitope Map
Author Location	Pol(330-338)
Epitope	`NPEIVIYQY`	Epitope Alignment
Epitope Name	NY9
Species (MHC/HLA)	human(C*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
NPEIVIYQY had a significantly positive association with HLA C*07; it was recognized by 7/94 C*07-positive subjects and 1/155 C*07-negative subjects.