Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 673

Download this epitope record as JSON.

HXB2 Location	gp160(156-165) gp120(156-165) DNA(6690..6719)	gp160 Epitope Map
Author Location	gp120(156-165)
Epitope	NCSFNISTSI	Epitope Alignment
Species (MHC/HLA)	human(C*08)
Immunogen	HIV-1 infection
Experimental methods
Keywords	epitope processing

Notes

• Recognized by CTL clone LWF A5, isolated from a lab worker exposed to HIV-1 in 1985.
• The processing of this epitope is TAP1/2-dependent, as are most Env epitopes, and it contains two N-linked glycosylation sites that are glycosylated in Env.
• Only peptide that has been deglycosylated, a process that changes asparagine (N) to aspartic acid (D) was recognized: the aspartic acid at position 5 was critical, position 1 could be either D or N.
• This peptide also contains a Cys involved in a disulfide linkage but reducing conditions did not affect recognition by CTL clone LWF A5.
• The HIV-1 Env epitopes are typically processed by a TAP1/2 dependent mechanism, which involves cotranslational translocation into the ER, glycosylation, export back into the cytosol, and deglycosylation for processing, and retransport into the ER for the association with class I molecules.
• The particular pathway of generating an epitope may have an impact on the presentation of that epitope, quantitatively as well as qualitatively.

References

Ferris1999 R. L. Ferris, C. Hall, N. V. Sipsas, J. T. Safrit, A. Trocha, R. A. Koup, R. P. Johnson, and R. F. Siliciano. Processing of HIV-1 Envelope Glycoprotein for Class I-Restricted Recognition: Dependence on TAP1/2 and Mechanisms for Cytosolic Localization. J. Immunol., 162:1324-1332, 1999. PubMed ID: 9973386. Show all entries for this paper.

Displaying record number 672

Download this epitope record as JSON.

HXB2 Location	gp160(156-165) gp120(156-165) DNA(6690..6719)	gp160 Epitope Map
Author Location	gp120(156-165 IIIB)
Epitope	NCSFNISTSI	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• HIV IIIB proteins were used to define the range of CTL epitopes recognized by 3 lab workers accidentally infected with HIV-1 IIIB.
• NCSFNITTSI, a variant found in HIV-1 MN, was not recognized, thus this epitope was type-specific.
• NCSFNISTSI contains two potential N-linked glycosylation sites and cysteine residue, possibly related to the requirement for a high sensitizing dose of peptide for CTL activity.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.

Displaying record number 52213

Download this epitope record as JSON.

HXB2 Location	gp160(156-165) gp120(156-165) DNA(6690..6719)	gp160 Epitope Map
Author Location	Env(162-171 BH10, LAI)
Epitope	NCSFNISTSI	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	computer prediction
Experimental methods	Other
Keywords	computational epitope prediction, T-cell crossreactivity to human proteins

Notes

• This study employs an antigenic similarity matrix to compare HIV-1 antigenic determinants with human proteins.
• This CTL epitope (the HIV-1 LAI fragment with high similarity to a human protein overlapping this epitope is STSIRGKVQK) has similarity with the macrophage colony stimulating factor I receptor fragment SISIRLKVQK.

References

Maksiutov2002 A. Z. Maksiutov, A. G. Bachinskii, and S. I. Bazhan. [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459, May-Jun 2002. Article in Russian. PubMed ID: 12068630. Show all entries for this paper.