Search CTL/CD8+ T-Cell Epitope Database

Found 11 matching records:

Displaying record number 54118

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	p17(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Species (MHC/HLA)	human(A*30)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes that this is an A*30 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 60015

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	Gag(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11
Species (MHC/HLA)	human(A*30)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LVWASRELERF had a significantly positive association with HLA A*30; it was recognized by 2/14 A*30-positive subjects and 1/143 A*30-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 55945

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	p17
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11(p17)
Subtype	B
Species (MHC/HLA)	human(A30)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Previously described HLA-A30-restricted epitope LVWASRELERF elicited an immune response in Chinese HIV-1 positive subjects as part of peptide KHLVWASRELERFAV.
1 of the 15 HLA-A30 carriers responded to LVWASRELERF-containing peptide with a magnitude of CTL response of 260 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 53056

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	p17
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF-11
Subtype	C
Species (MHC/HLA)	human(A30:02, B57:03)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining
Keywords	subtype comparisons, epitope processing, immunodominance, HLA cross-presentation

Notes

Highly targeted regions in Gag for CD8+ T-cells were defined for individuals with C clade infections in South Africa. 9 specific epitopes within the most reactive regions were characterized. This is 1 of 5 novel epitopes that were found among subtype C HIV-1 from African patients who hadn't previously been identified in B clade infections. Some epitopes were shown to be promiscuous, presented by multiple class I restricting alleles.
LVWASRELERF was clearly presented by both A*3002 and B*570301, it might also be cross-presented by A*3001, but not as effectively. A*30 is 10-fold more common among Zulus than Caucasians (allele frequency 0.195 versus 0.019), while B*57 is similar (0.051 versus 0.043).

References

Masemola2004a Agatha M. Masemola, Tumelo N. Mashishi, Greg Khoury, Helba Bredell, Maria Paximadis, Tiyani Mathebula, Debra Barkhan, Adrian Puren, Efthyia Vardas, Mark Colvin, Lynn Zijenah, David Katzenstein, Rosemary Musonda, Susan Allen, Newton Kumwenda, Taha Taha, Glenda Gray, James McIntyre, Salim Abdool Karim, Haynes W. Sheppard, and Clive M. Gray. Novel and Promiscuous CTL Epitopes in Conserved Regions of Gag Targeted by Individuals with Early Subtype C HIV Type 1 Infection from Southern Africa. J. Immunol., 173(7):4607-4617, 1 Oct 2004. PubMed ID: 15383595. Show all entries for this paper.

Displaying record number 60016

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	Gag(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11
Species (MHC/HLA)	human(B*52)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LVWASRELERF had a significantly positive association with HLA B*52; it was recognized by 2/9 B*52-positive subjects and 3/157 B*52-negative subjects.

References

Displaying record number 60013

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	Gag(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11
Species (MHC/HLA)	human(B*57)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LVWASRELERF had a significantly positive association with HLA B*57; it was recognized by 22/94 B*57-positive subjects and 5/166 B*57-negative subjects.

References

Displaying record number 53307

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`LVWASRELERF`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 58693

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	p17(81-89)
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11
Subtype	C
Species (MHC/HLA)	human(B*57:03)
Immunogen	HIV-1 infection
Country	Botswana, South Africa, Zimbabwe
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Intracellular cytokine staining, Tetramer binding
Keywords	optimal epitope, HLA associated polymorphism, novel epitope

Notes

1,010-HIV C clade-chronically infected subjects were tested in order to distinguish levels of immune control by related but different HLA-B*57/58. B*57 micropolymorphisms (B*5703 and B*5702) as well as B*5801 have differing CTL specificity and also present different p24 Gag epitopes. This results in different immune selection pressures on the HIV-C virus.
Though all three studied HLA alleles, B*5801, B*5702 and B*5703 are significantly associated with lower viral-load (VL) set points, HLA-B*5703, followed closely by B*5702 is the most potent controller with lowest VL and HLA-B*5702, followed by B*5703 and then B*5801 are associated with highest CD4 counts.
By using 410 overlapping 18-mers that span the HIV-C proteome in EliSpot assays, 17 epitopes were identified that are presented by one of HLAs-B*5801/5702/5703, 13 of them novel.
Novel epitope LF11, LVWASRELERF, was confirmed to be optimal by peptide truncation and restricted by HLA-B*5703 using CTL from subject R014.
The binding affinity and stability of LF11 is greatest to B*5703.
˜50% B*57-positive and non-B*57-subjects carried mutations in LF11.

References

Kloverpris2012 Henrik N. Kloverpris, Anette Stryhn, Mikkel Harndahl, Mary van der Stok, Rebecca P. Payne, Philippa C. Matthews, Fabian Chen, Lynn Riddell, Bruce D. Walker, Thumbi Ndung'u, Søren Buus, and Philip Goulder. HLA-B*57 Micropolymorphism Shapes HLA Allele-Specific Epitope Immunogenicity, Selection Pressure, and HIV Immune Control. J. Virol., 86(2):919-929, Jan 2012. PubMed ID: 22090105. Show all entries for this paper.

Displaying record number 53958

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`LVWASRELERF`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*57:03)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
LVWASRELERF is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 60014

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	Gag(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Epitope Name	LF11
Species (MHC/HLA)	human(C*04)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LVWASRELERF had a significantly positive association with HLA C*04; it was recognized by 21/81 C*04-positive subjects and 27/260 C*04-negative subjects.

References

Displaying record number 62774

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HXB2 Location	Gag(34-44) p17(34-44) DNA(889..921)	Gag Epitope Map
Author Location	p17(34-44)
Epitope	`LVWASRELERF`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 69 single nucleotide mutations were observed in epitope LVWASRELERF (known to bind to A30) with a log transformation of mean fitness score, relative to NL4-3, of -0.5952.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.