Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 53989

Download this epitope record as JSON.

HXB2 Location	Pol(132-141) Protease(76-85) DNA(2478..2507)	Pol Epitope Map
Author Location	( C consensus)
Epitope	LVGPTPVNII	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*02:05)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

• A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
• LVGPTPVNII is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 57780

Download this epitope record as JSON.

HXB2 Location	Pol(132-141) Protease(76-85) DNA(2478..2507)	Pol Epitope Map
Author Location	( H2d)
Epitope	LVGPTPVNII	Epitope Alignment
Species (MHC/HLA)	mouse, transgenic mouse
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, T-cell Elispot
Keywords	vaccine-induced immune responses, variant cross-reactivity, drug resistance

Vaccine Details

Notes

• DNA plasmids encoding protease variants were designed. D25N mutation drastically reduced the enzymatic activity of the protease and at the same time increased expression and immunogenicity of the protease.
• Response against the protease gene variants was reduced when the cells were stimulated with peptides carrying the V82F/I84V mutations, located in previously decribed VGPTPvNiI epitope. the 82/84 mutations destroyed the dominant VGPTPvNiI epitope, as well as a previously unknown CD4+ T helper epitope PvNiIGRNLLTQIGC.
• LVGPTPVNII is one of the three peptides overlapping the previously described H2d epitope VGPTPVNII, that stimulated the strongest immune responses.

References

Hallengard2011 David Hallengärd, B. Kristian Haller, Sarah Petersson, Andreas Boberg, Anna-Karin Maltais, Maria Isaguliants, Britta Wahren, and Andreas Bråve. Increased Expression and Immunogenicity of HIV-1 Protease Following Inactivation of the Enzymatic Activity. Vaccine, 29(4):839-348, 17 Jan 2011. PubMed ID: 21109032. Show all entries for this paper.