Search CTL/CD8+ T-Cell Epitope Database

Found 12 matching records:

Displaying record number 1309

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef( 221A)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	Nef-221a
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	binding affinity, supertype, computational epitope prediction, HLA cross-presentation

Notes

HIV was scanned for all peptides which carried the A2-supermotif pattern conserved in more than 50% of B clade sequences -- 233 peptides met this criteria, and 30 of these bound to HLA-A*0201 -- 20/30 bound to at least 3/5 of HLA-A2 supertype alleles tested.
Three additional previously described HLA-A2 epitopes were added to the set of 20, and 18/22 chronically infected HLA-A2 individuals had CTL that recognized at least one of the 23 peptides (median of 2 and maximum of 6), while 6/12 acute infected individuals recognized at least 1 (median of 1 and maximum of 2)
1/22 individuals with chronic HIV-1 infection recognized this epitope in ELISPOT.
2/12 acutely infected individuals recognized this epitope.
LTFGWCFKLV binds to five HLA-A2 supertype alleles: A*0203, A*0201 (highest affinity), A*0206, A*6802 and A*0202.

References

Altfeld2001 M. A. Altfeld, B. Livingston, N. Reshamwala, P. T. Nguyen, M. M. Addo, A. Shea, M. Newman, J. Fikes, J. Sidney, P. Wentworth, R. Chesnut, R. L. Eldridge, E. S. Rosenberg, G. K. Robbins, C. Brander, P. E. Sax, S. Boswell, T. Flynn, S. Buchbinder, P. J. Goulder, B. D. Walker, A. Sette, and S. A. Kalams. Identification of novel HLA-A2-restricted human immunodeficiency virus type 1-specific cytotoxic T-lymphocyte epitopes predicted by the HLA-A2 supertype peptide-binding motif. J. Virol., 75(3):1301-11, Feb 2001. URL: http://jvi.asm.org/cgi/content/full/75/3/1301. PubMed ID: 11152503. Show all entries for this paper.

Displaying record number 53487

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords

Notes

4/14 optimal HIV-1 T-cell epitopes in a subject underwent mutation associated with dramatic loss of the original CD8 response. For 1 of the escape variants, a novel CD8 T-cell response equal in magnitude to the wildtype, was generated. CD8 T-cell recognizing the variant epitope utilized a distinct T-cell receptor and did not exhibit any cross-reactivity against the wildtype.
This epitope did not vary.

References

Allen2005 Todd M. Allen, Xu G. Yu, Elizabeth T. Kalife, Laura L. Reyor, Mathias Lichterfeld, Mina John, Michael Cheng, Rachel L. Allgaier, Stanley Mui, Nicole Frahm, Galit Alter, Nancy V. Brown, Mary N. Johnston, Eric S. Rosenberg, Simon A. Mallal, Christian Brander, Bruce D. Walker, and Marcus Altfeld. De Novo Generation of Escape Variant-Specific CD8+ T-Cell Responses Following Cytotoxic T-Lymphocyte Escape in Chronic Human Immunodeficiency Virus Type 1 Infection. J. Virol., 79(20):12952-12960, Oct 2005. PubMed ID: 16188997. Show all entries for this paper.

Displaying record number 53513

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	escape, TCR usage, variant cross-reactivity, characterizing CD8+ T cells

Notes

4/14 optimal HIV-1 T-cell epitopes in a subject underwent mutation associated with dramatic loss of the original CD8 response. For 1 of the escape variants, a novel CD8 T-cell response equal in magnitude to the wildtype, was generated. CD8 T-cell recognizing the variant epitope utilized a distinct T-cell receptor and did not exhibit any cross-reactivity against the wildtype.
This epitope did not vary over time.

References

Displaying record number 53563

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
This epitope was reactive, but escape mutations did not accrue in it over time.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 53581

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(135-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, HLA binding
Keywords	acute/early infection, optimal epitope

Notes

The most frequently targeted HLA-A*02-restricted CD8+ T-cell epitopes in chronic infection were significantly less frequently recognized during primary infection. This epitope was only recognized during chronic infection.

References

Altfeld2005a Marcus Altfeld, Todd M. Allen, Elizabeth T. Kalife, Nicole Frahm, Marylyn M. Addo, Bianca R. Mothe, Almas Rathod, Laura L. Reyor, Jason Harlow, Xu G. Yu, Beth Perkins, Loren K. Robinson, John Sidney, Galit Alter, Mathias Lichterfeld, Alessandro Sette, Eric S. Rosenberg, Philip J. R. Goulder, Christian Brander, and Bruce D. Walker. The Majority of Currently Circulating Human Immunodeficiency Virus Type 1 Clade B Viruses Fail To Prime Cytotoxic T-Lymphocyte Responses against an Otherwise Immunodominant HLA-A2-Restricted Epitope: Implications for Vaccine Design. J. Virol., 79(8):5000-5005, Apr 2005. PubMed ID: 15795285. Show all entries for this paper.

Displaying record number 55016

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef
Epitope	`LTFGWCFKLV`	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope LTFGWCFKLV elicited a magnitude of response of 60 SFC with a functional avidity of 0.05nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 60002

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Species (MHC/HLA)	human(A*23)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LTFGWCFKLV had a significantly positive association with HLA A*23; it was recognized by 5/22 A*23-positive subjects and 5/263 A*23-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 1589

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(158-167)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Species (MHC/HLA)	human(A2 supertype)
Immunogen	HIV-1 infection
Experimental methods
Keywords	supertype, rate of progression

Notes

Long-term nonprogressors (LTNPs) had strong memory resting CD8+ T-cell responses against the majority of epitopes tested, (18 for the A2 supertype, 16 for the A3 supertype) while the effector cells of long-term nonprogressors recognized far fewer epitopes.
Progressors had memory resting CD8+ T-cells that recognized far fewer epitopes than LTNPs.
A positive correlation between effector CD8+ T-cells and plasma viremia and a negative correlation between CD8+ effector T-cells and CD4+ T-cells was observed, which may contribute to the inability of LTNPs to clear virus.
This epitope can bind all five HLA-A2 supertypes alleles (A*0201, A*0202, A*0203, A*0206 and A*6802)
Tetramer staining with A2, beta2microglobulin, and either SLYNTVATL, KLVGKLNWA, or LTFGWCFKL revealed that tetramers detected more HIV-specific sells in LTNP than in progressors, activated effector cells were the minority population, and ELISPOT correlated better with the effector cell subpopulation than the total tetramer stained population.

References

Propato2001 A. Propato, E. Schiaffella, E. Vicenzi, V. Francavilla, L. Baloni, M. Paroli, L. Finocchi, N. Tanigaki, S. Ghezzi, R. Ferrara, R. Chesnut, B. Livingston, A. Sette, R. Paganelli, F. Aiuti, G. Poli, and V. Barnaba. Spreading of HIV-specific CD8+ T-cell repertoire in long-term nonprogressors and its role in the control of viral load and disease activity. Hum. Immunol., 62(6):561-76, Jun 2001. PubMed ID: 11390031. Show all entries for this paper.

Displaying record number 60003

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Species (MHC/HLA)	human(A*32)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LTFGWCFKLV had a significantly positive association with HLA A*32; it was recognized by 8/30 A*32-positive subjects and 16/311 A*32-negative subjects.

References

Displaying record number 60004

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LTFGWCFKLV had a significantly positive association with HLA A*6802; it was recognized by 6/26 A*6802-positive subjects and 10/285 A*6802-negative subjects.

References

Displaying record number 60006

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Species (MHC/HLA)	human(A*80)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LTFGWCFKLV had a significantly positive association with HLA A*80; it was recognized by 1/1 A*80-positive subjects and 4/262 A*80-negative subjects.

References

Displaying record number 60005

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HXB2 Location	Nef(137-146) DNA(9205..9234)	Nef Epitope Map
Author Location	Nef(137-146)
Epitope	`LTFGWCFKLV`	Epitope Alignment
Epitope Name	LV10
Species (MHC/HLA)	human(B*15:17)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LTFGWCFKLV had a significantly positive association with HLA B*1517; it was recognized by 1/1 B*1517-positive subjects and 3/261 B*1517-negative subjects.