Search CTL/CD8+ T-Cell Epitope Database

Found 12 matching records:

Displaying record number 55022

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location
Epitope	`LFCASDAKAY`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope LFCASDAKAY elicited a magnitude of response of 240 SFC with a functional avidity of 5nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 56431

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY10(gp120)
Subtype	B
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-gamma assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Although the tested peptide sequence (VQKEATTTLFCASDAKAY) contains the exact sequence of a previously described HLA-A24 optimal epitope, LFCASDAKAY, none of the 30 HLA-A24 carriers responded to it (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 1661

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120(53-62)
Epitope	`LFCASDAKAY`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 53013

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120(53-62)
Epitope	`LFCASDAKAY`	Epitope Alignment
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, CD8 T-cell Elispot granzyme B, T-cell Elispot
Keywords	characterizing CD8+ T cells

Notes

Only 20% of CD8+ T-cells produce IFN-gamma and granzyme B simultaneously (Tc1a). Two additional subpopulations of HIV specific CD8 cells are found, each one constituting 30-40% of the CD8 cell pool. One of these (Tc1b) secretes IFN-gamma only, and the other one (Tc1c) secretes GzB only.
Two of seven patients responded to this peptide with GzB producing cells, and a different patient with IFN-gamma producing cells.

References

Kleen2004 Thomas O. Kleen, Robert Asaad, Samuel J. Landry, Bernhard O. Boehm, and Magdalena Tary-Lehmann. Tc1 Effector Diversity Shows Dissociated Expression of Granzyme B and Interferon-gamma in HIV Infection. AIDS, 18(3):383-392, 20 Feb 2004. PubMed ID: 15090789. Show all entries for this paper.

Displaying record number 54263

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	A24-LY10(gp120)
Species (MHC/HLA)	human(A24)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contribution of immune responses restricted to individual HLA class I molecules were studied.
The most frequently recognised epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contribution of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 645

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120(59-68 HXB2)
Epitope	`LFCASDAKAY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*2402)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL epitope defined by T cell line and peptide mapping.
C. Brander notes that this is an A*2402 epitope in the 1999 database.

References

Lieberman1992 J. Lieberman, J. A. Fabry, M.-C. Kuo, P. Earl, B. Moss, and P. R. Skolnik. Cytotoxic T Lymphocytes from HIV-1 Seropositive Individuals Recognize Immunodominant Epitopes in gp160 and Reverse Transcriptase. J. Immunol., 148:2738-2747, 1992. This paper does not use T-cell clones to map epitopes, but rather T-cell lines from HIV infected donors. 20 amino acid peptides were used to map the region of the reactive epitopes. HLA restriction was not tested for all epitopes. PubMed ID: 1374097. Show all entries for this paper.

Displaying record number 1130

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	gp120(53-62 LAI)
Epitope	`LFCASDAKAY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*2402)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is an A*2402 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 59982

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	Env(52-61)
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LFCASDAKAY had a significantly positive association with HLA B*07; it was recognized by 8/34 B*07-positive subjects and 14/257 B*07-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59983

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	Env(52-61)
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(B*08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LFCASDAKAY had a significantly positive association with HLA B*08; it was recognized by 3/19 B*08-positive subjects and 4/203 B*08-negative subjects.

References

Displaying record number 59981

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	Env(52-61)
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LFCASDAKAY had a significantly positive association with HLA C*12; it was recognized by 12/50 C*12-positive subjects and 22/291 C*12-negative subjects.

References

Displaying record number 59980

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	Env(52-61)
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(C*16)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LFCASDAKAY had a significantly positive association with HLA C*16; it was recognized by 7/35 C*16-positive subjects and 7/222 C*16-negative subjects.

References

Displaying record number 57116

HXB2 Location	gp160(52-61) gp120(52-61) DNA(6378..6407)	gp160 Epitope Map
Author Location	Env(52-61 consensus)
Epitope	`LFCASDAKAY`	Epitope Alignment
Epitope Name	LY9
Subtype	A, B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	A0201, A0301, B0702, B1801, Cw0702, Cw1203
Country	Spain
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

Virus isolates were tested for clade, drug susceptibility, coreceptor use, and growth rate in 2 case reports involving transmission pairs that included a source patient and an index patient each. Primary HIV-1 infections with severe clinical symptoms were documented in index patients. Both index cases mounted only a few, relatively weak cellular immune responses.
Peptide LFCASDAKAY was recognized by index patient 2, who was recently infected by HIV-1 subtype CRF02.

References

Dalmau2009 Judith Dalmau, Maria Carmen Puertas, Marta Azuara, Ana Mariño, Nicole Frahm, Beatriz Mothe, Nuria Izquierdo-Useros, Maria José Buzón, Roger Paredes, Lourdes Matas, Todd M. Allen, Christian Brander, Carlos Rodrigo, Bonaventura Clotet, and Javier Martinez-Picado. Contribution of Immunological and Virological Factors to Extremely Severe Primary HIV Type 1 Infection. Clin. Infect. Dis., 48(2):229-238, 15 Jan 2009. PubMed ID: 19093810. Show all entries for this paper.