Search CTL/CD8+ T-Cell Epitope Database

Found 9 matching records:

Displaying record number 53562

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-110)
Epitope	`LADQLIHLHY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B18)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
This epitope was reactive, but escape mutations did not accrue in it over time.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 53483

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-110)
Epitope	`LADQLIHLHY`	Epitope Alignment
Epitope Name	LY10
Subtype	B
Species (MHC/HLA)	human(B18)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A2, B18, B44, C*12, Cw5
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords

Notes

4/14 optimal HIV-1 T-cell epitopes in a subject underwent mutation associated with dramatic loss of the original CD8 response. For 1 of the escape variants, a novel CD8 T-cell response equal in magnitude to the wildtype, was generated. CD8 T-cell recognizing the variant epitope utilized a distinct T-cell receptor and did not exhibit any cross-reactivity against the wildtype.
This epitope did not vary.

References

Allen2005 Todd M. Allen, Xu G. Yu, Elizabeth T. Kalife, Laura L. Reyor, Mathias Lichterfeld, Mina John, Michael Cheng, Rachel L. Allgaier, Stanley Mui, Nicole Frahm, Galit Alter, Nancy V. Brown, Mary N. Johnston, Eric S. Rosenberg, Simon A. Mallal, Christian Brander, Bruce D. Walker, and Marcus Altfeld. De Novo Generation of Escape Variant-Specific CD8+ T-Cell Responses Following Cytotoxic T-Lymphocyte Escape in Chronic Human Immunodeficiency Virus Type 1 Infection. J. Virol., 79(20):12952-12960, Oct 2005. PubMed ID: 16188997. Show all entries for this paper.

Displaying record number 52561

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111)
Epitope	`LADQLIHLHY`	Epitope Alignment
Species (MHC/HLA)	human(B*18:01)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61693

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111)
Epitope	`LADQLIHLHY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(B*18:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	review

Notes

This review makes the argument that T-cell subsets recognizing the HIV/SIV viral infectivity factor Vif, may be essential for control of HIV replication as vif-deficient strains can only complete one round of replication and Vif targets the cellular viral-restriction-factor, APOBEC3G (A3G) with the help of Elongin B/C and Cullin-5 to proteasomal degradation.
HIV-1 HLA-B*1801 restricted epitope LY10, LADQLIHLHY (Vif 102-111) does not overlap any Vif functional domains.

References

Silver2017 Zachary A. Silver and David I. Watkins. The Role of MHC Class I Gene Products in SIV Infection of Macaques. Immunogenetics, 69(8-9):511-519, Aug 2017. PubMed ID: 28695289. Show all entries for this paper.

Displaying record number 52404

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111)
Epitope	`LADQLIHLHY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*18:01)
Immunogen	HIV-1 infection
Experimental methods
Keywords	early-expressed proteins

Notes

CTL responses against regulatory and accessory proteins in 70 HIV-1 infected patients were studied using overlapping peptides and Elispot.
28% targeted one or more overlapping Tat peptides; 36%, Rev peptides; 33%, Vif peptides; 40%, Vpr peptides; and 2%, Vpu peptides.
All known optimally defined epitopes were summarized for the five proteins.

References

Addo2002a M. M. Addo, X. G. Yu, E. S. Rosenberg, B. D. Walker, and M. Altfeld. Cytotoxic T-Lymphocyte (CTL) Responses Directed against Regulatory and Accessory Proteins in HIV-1 Infection. DNA Cell Biol., 21(9):671-678, Sep 2002. PubMed ID: 12396610. Show all entries for this paper.

Displaying record number 1270

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111 SF2)
Epitope	`LADQLIHLHY`	Epitope Alignment
Species (MHC/HLA)	human(B*18:01)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

CTL responses against HIV-1 Vpr, Vpu, and Vif were analyzed in multiple HIV-1-infected individuals.
10/29 (35%) individuals tested responded to Vif.
This epitope was recognized by 2/5 individuals expressing B*1801 allele.

References

Altfeld2001c M. Altfeld, M. M. Addo, R. L. Eldridge, X. G. Yu, S. Thomas, A. Khatri, D. Strick, M. N. Phillips, G. B. Cohen, S. A. Islam, S. A. Kalams, C. Brander, P. J. Goulder, E. S. Rosenberg, B. D. Walker, and The HIV Study Collaboration.. Vpr is preferentially targeted by CTL during HIV-1 infection. J. Immunol., 167(5):2743-52, 1 Sep 2001. URL: http://www.jimmunol.org/cgi/content/full/167/5/2743. PubMed ID: 11509618. Show all entries for this paper.

Displaying record number 59978

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111)
Epitope	`LADQLIHLHY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(B*45)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LADQLIHLHY had a significantly positive association with HLA B*45; it was recognized by 3/11 B*45-positive subjects and 20/294 B*45-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59977

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location	Vif(102-111)
Epitope	`LADQLIHLHY`	Epitope Alignment
Epitope Name	LY10
Species (MHC/HLA)	human(C*05)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
LADQLIHLHY had a significantly positive association with HLA C*05; it was recognized by 23/36 C*05-positive subjects and 23/305 C*05-negative subjects.

References

Displaying record number 52060

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HXB2 Location	Vif(102-111) DNA(5344..5373)	Vif Epitope Map
Author Location
Epitope	`LADQLIHLHY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords	epitope processing, escape

Notes

Epitopes that have been described in the literature and included in this database tend to cluster in conserved regions and be absent or rarely found in highly variable regions in Nef, Env, and p17.
While the uneven distribution of epitopes may be in part due to a limited cross-recognition of specific responses because of differences between peptides used to probe the immune response and autologous strains, regions with a paucity of defined epitopes also had higher frequencies of amino acids that tend to not be found in C-terminal positions of epitopes, and had lower cleavage prediction scores for epitope processing. This suggests that in the regions of the virus where variation is best tolerated, traces of immune escape have left an imprint on the viral population. Epitopes also were concentrated in alpha-helix and turn regions in the proteins.
In the more conserved p24, and Pol proteins RT and Protease, epitopes are more evenly distributed.
What was learned from proteins where many epitopes have been defined (Gag, Pol, Env and Nef) was used to develop an algorithm to predict where epitopes would be localized in Rev, Tat, Vif, and Vpr. Predictions were made blinded, and then compared to the first 15 epitopes defined in these proteins; the epitopes were concentrated in the predicted regions.

References

Yusim2002 Karina Yusim, Can Kesmir, Brian Gaschen, Marylyn M. Addo, Marcus Altfeld, S\oren Brunak, Alexandre Chigaev, Vincent Detours, and Bette T. Korber. Clustering Patterns of Cytotoxic T-Lymphocyte Epitopes in Human Immunodeficiency Virus Type 1 (HIV-1) Proteins Reveal Imprints of Immune Evasion on HIV-1 Global Variation. J. Virol., 76(17):8757-8768, Sep 2002. PubMed ID: 12163596. Show all entries for this paper.