Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 59968

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	Env(794-802)
Epitope	`KYCWNLLQY`	Epitope Alignment
Epitope Name	KQY9
Species (MHC/HLA)	human(A*30)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KYCWNLLQY had a significantly positive association with HLA A*30; it was recognized by 10/53 A*30-positive subjects and 18/288 A*30-negative subjects.
KYCWNLLQY was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-A*30 in a cohort of 245 controllers and 96 progressors.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 54273

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	Env
Epitope	`KYCWNLLQY`	Epitope Alignment
Epitope Name	A30-KQY9(env)
Species (MHC/HLA)	human(A30)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contribution of immune responses restricted to individual HLA class I molecules were studied.
The most frequently recognised epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contribution of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 57220

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	Env
Epitope	`KYCWNLLQY`	Epitope Alignment
Species (MHC/HLA)	(A30)
Immunogen	HIV-1 infection
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	mother-to-infant transmission

Notes

HIV-1 peptide-specific IFN-γ responses were measured over the first year of life for vertically-infected infants. Late-infected infants (between months 1 and 12) had responses that were more rapid and more focused than those infected before 1 month of life.
1/3 infants tested recognized KYCWNLLQY.

References

Lohman-Payne2009 B. Lohman-Payne, J. A. Slyker, B. A. Richardson, C. Farquhar, M. Majiwa, E. Maleche-Obimbo, D. Mbori-Ngacha, J. Overbaugh, S. Rowland-Jones, and G. John-Stewart. Infants with Late Breast Milk Acquisition of HIV-1 Generate Interferon-gamma Responses More Rapidly than Infants with Early Peripartum Acquisition. Clin. Exp. Immunol., 156(3):511-517, Jun 2009. PubMed ID: 19438605. Show all entries for this paper.

Displaying record number 1198

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	gp160(794-802 LAI)
Epitope	`KYCWNLLQY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*3002)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is an A*3002 epitope.

References

Goulder2001 P. J. Goulder, M. M. Addo, M. A. Altfeld, E. S. Rosenberg, Y. Tang, U. Govender, N. Mngqundaniso, K. Annamalai, T. U. Vogel, M. Hammond, M. Bunce, H. M. Coovadia, and B. D. Walker. Rapid Definition of Five Novel HLA-A*3002-Restricted Human Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Epitopes by Elispot and Intracellular Cytokine Staining Assays. J. Virol., 75(3):1339-1347, Feb 2001. URL: http://jvi.asm.org/cgi/content/full/75/3/1339. PubMed ID: 11152507. Show all entries for this paper.

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 1764

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	gp41
Epitope	`KYCWNLLQY`	Epitope Alignment
Epitope Name	KY9 (gp41)
Species (MHC/HLA)	human(A*3002)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

HLA-A*3002 is very common in African populations, 50% of Zimbabweans express HLA-A30, 44% in African Zulu, so five new HIV epitopes were characterized that are presented by this HLA molecule.
A rapid method was developed combining ELISPOT with intracellular IFN-γ staining of PBMCs to map optimal epitopes, then HLA presenting molecules were defined -- this method was completed within 48 to 72 hours of receipt of blood.
Two individuals were studied: Subject 199 (HLA A*0201/*3002 B*4402/51 Cw2/5), a Caucasian, and Subject 6007 (HLA A*3002/ B53/*5801 Cw4/7) an African-Caribbean.
In both HLA-A*3002 individuals the response to RSLYNTVATLY was dominant.
In subject 199 four additional A*3002 epitopes were identified.
Three quantitative assays, ELISPOT, precursor frequency and chromium release, confirmed a hierarchy of response: RY11 (p17) > KY9 (gp41) > KY9 (RT-53) > IY9 (gp41)

References

Displaying record number 52541

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	gp41(283-291)
Epitope	`KYCWNLLQY`	Epitope Alignment
Species (MHC/HLA)	human(A*3002)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

References

Displaying record number 59969

HXB2 Location	gp160(794-802) gp41(283-291) DNA(8604..8630)	gp160 Epitope Map
Author Location	Env(794-802)
Epitope	`KYCWNLLQY`	Epitope Alignment
Epitope Name	KQY9
Species (MHC/HLA)	human(C*16)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KYCWNLLQY had a significantly positive association with HLA C*16; it was recognized by 5/31 C*16-positive subjects and 13/257 C*16-negative subjects.
KYCWNLLQY was significantly nonprotective, associated with HIV chronic disease progression when restricted by HLA-C*16 in a cohort of 245 controllers and 96 progressors.