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Displaying record number 56877

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HXB2 Location	Nef(82-90) DNA(9040..9066)	Nef Epitope Map
Author Location
Epitope	KSALDLSHF	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B57)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	escape, elite controllers

Notes

• The study examined Nef-specific CTL responses in 9 elite controllers who were B57-positive. EliSpot responses were measured for overlapping peptides spanning all of Nef. Of the total of 22 responses, 5 were confirmed to be against previously-defined B57-restricted optimal epitopes.
• Mutations in Nef sequences amplified from peripheral blood CD4+ T cells and from plasma virus were compared. Some Nef mutations were present in plasma visru but were rare in the cellular sequences and were shown to represent novel escape variants.
• 3 elite controllers (ES2, ES3, ES8) had a pattern of distinct substitutions present in plasma clones but not the proviral clones in KF9 epitope KAAVDLSHF epitope.
• KSALDLSHF was the proviral variant in Patient ES8, which elicited a stronger IFN-γ response than the plasma variant taALDmSHF, indicating that taALDmSHF is an escape variant.

References

Bailey2009 Justin R. Bailey, Timothy P. Brennan, Karen A. O'Connell, Robert F. Siliciano, and Joel N. Blankson. Evidence of CD8+ T-Cell-Mediated Selective Pressure on Human Immunodeficiency Virus Type 1 nef in HLA-B*57+ elite suppressors. J. Virol., 83(1):88-97, Jan 2009. PubMed ID: 18945771. Show all entries for this paper.