Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 57297

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HXB2 Location	Gag(263-274) p24(131-142) DNA(1576..1611)	Gag Epitope Map
Author Location	p24(131-142)
Epitope	`KRWIILGLNKIV`	Epitope Alignment
Epitope Name	KV12
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	peptide-HLA interaction
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Other
Keywords	epitope processing

Notes

Proteasomal cleavage effects on immunodominance were studied using 8 p17 and 11 p24 peptide variants. Epitope abundance due to Antigen processing was the prime influence on CTL dominance while HLA affinity was a moderate influence. Viral mutations modified processing [proteasomal digestion, transporter protein (TAP) affinity, Endoplasmic Reticulum Aminopeptidase (ERAAP) trimming].
Immunodominant epitope, KK12, KRWIILGLNKIV, was produced by proteasomal cleavage and elicited CTL response.

References

Tenzer2009 Stefan Tenzer, Edmund Wee, Anne Burgevin, Guillaume Stewart-Jones, Lone Friis, Kasper Lamberth, Chih-hao Chang, Mikkel Harndahl, Mirjana Weimershaus, Jan Gerstoft, Nadja Akkad, Paul Klenerman, Lars Fugger, E. Yvonne Jones, Andrew J. McMichael, Søren Buus, Hansjörg Schild, Peter van Endert, and Astrid K. N. Iversen. Antigen Processing Influences HIV-Specific Cytotoxic T Lymphocyte Immunodominance. Nat. Immunol., 10(6):636-646, Jun 2009. PubMed ID: 19412183. Show all entries for this paper.

Displaying record number 288

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HXB2 Location	Gag(263-274) p24(131-142) DNA(1576..1611)	Gag Epitope Map
Author Location	p24(265-276)
Epitope	`KRWIILGLNKIV`	Epitope Alignment
Species (MHC/HLA)	human(B27)
Immunogen	peptide-HLA interaction
Experimental methods
Keywords

Notes

Epitope examined in the context of peptide binding to HLA-B27.

References

Jardetzky1991 T. S. Jardetzky, W. S. Lane, R. A. Robinson, D. R. Madden, and D. C. Wiley. Identification of Self Peptides Bound to Purified HLA-B27. Nature, 353:326-329, 1991. A pool of endogenous peptides bound to the human class I MHC molecule, HLA-B27, has been isolated. Microsequence analysis of the pool and of 11 HPLC-purified peptides provides information on the binding specificity of the HLA-B27 molecule. The peptides all seem to be nonamers, seven of which match to protein sequences in a database search. These self peptides derive from abundant cytosolic or nuclear proteins, such as histone, ribosomal proteins, and members of the 90K heat-shock protein family. PubMed ID: 1922338. Show all entries for this paper.

Displaying record number 289

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HXB2 Location	Gag(263-274) p24(131-142) DNA(1576..1611)	Gag Epitope Map
Author Location	p24(263-274 LAI)
Epitope	`KRWIILGLNKIV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	dendritic cells

Notes

The capacity of dendritic cells to process and present antigen and stimulate anti-HIV-1 CTL memory responses was studied.

References

Fan1997 Z. Fan, X. L. Huang, L. Zheng, C. Wilson, L. Borowski, J. Liebmann, P. Gupta, J. Margolick, and C. Rinaldo. Cultured Blood Dendritic Cells Retain HIV-1 Antigen-Presenting Capacity for Memory CTL During Progressive HIV-1 Infection. J. Immunol., 159:4973-4982, 1997. PubMed ID: 9366424. Show all entries for this paper.

Displaying record number 1454

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HXB2 Location	Gag(263-274) p24(131-142) DNA(1576..1611)	Gag Epitope Map
Author Location	p24(131-142)
Epitope	`KRWIILGLNKIV`	Epitope Alignment
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

One of the 51 HIV-1 epitopes selected by Ferrari et al. as good candidate CTL epitopes for vaccines by virtue of being conserved and presented by common HLA alleles.

References

Ferrari2000 G. Ferrari, D. D. Kostyu, J. Cox, D. V. Dawson, J. Flores, K. J. Weinhold, and S. Osmanov. Identification of highly conserved and broadly cross-reactive HIV type 1 cytotoxic T lymphocyte epitopes as candidate immunogens for inclusion in Mycobacterium bovis BCG-vectored HIV vaccines. AIDS Res. Hum. Retroviruses, 16(14):1433-43, 20 Sep 2000. PubMed ID: 11018863. Show all entries for this paper.

Displaying record number 60662

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HXB2 Location	Gag(263-274) p24(131-142) DNA(1576..1611)	Gag Epitope Map
Author Location	p24(131-142)
Epitope	`KRWIILGLNKIV`	Epitope Alignment
Epitope Name	KV-12
Subtype	B, C
Species (MHC/HLA)	human(B*27:05)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Subtype-specific substitutions in two HIV Gag regions (fragments F1, amino acids (aa) 5-46, and F2 aa 100-143) result in novel adaptations that affect epitope processing efficiency. Consequent low epitope abundance is associated with weak T cell response and greater immune escape and therefore with disease state. Conversely, epitopes from these regions when restricted by certain rare HLA I alleles, are associated with greater production and resulting CTL response, therefore delayed disease progression.
Most epitopes were found to be processed as precursors with N-terminal extensions beyond the optimal epitope. Only 4 epitopes in this region were associated with delayed progression - HLA-B*5701/03-restricted IW9 (ISPRTLNAW), KF11 (KAFSPEVIPMF), TW10 (TSTLQEQIGW) and HLA-B*2705-restricted KK10 (KRWIILGLNK).
Regardless of ethnic group, white or African, a significant three-way interaction was found between HLA allelic frequency, epitope yield and ethnicity for epitopes of N-terminal fragment F1 (NF1) and C-terminal as well as middle F2 (CF2 and MF2). C-terminal F1 (CF1) epitope yields however, showed a significant difference between ethnic groups. Also, for identical HLA-mediated selection pressure in both Africans and whites, HIV-C had a lower concentration than HIV-B, especially at lower HLA allelic frequencies.
Epitope KRWIILGLNKIV, KV-12, from fragments NF2-MF2 and CF2, - KK10 peptide form (Tenzer, S et al, Nature Immunology 2009), restricted by HLA-B*2705 (Boriz, E et al, Virology 2007), had no determined natural processed epitope forms for subtypes B or C.

References

Tenzer2014 Stefan Tenzer, Hayley Crawford, Phillip Pymm, Robert Gifford, Vattipally B. Sreenu, Mirjana Weimershaus, Tulio de Oliveira, Anne Burgevin, Jan Gerstoft, Nadja Akkad, Daniel Lunn, Lars Fugger, John Bell, Hansjörg Schild, Peter van Endert, and Astrid K. N. Iversen. HIV-1 Adaptation to Antigen Processing Results in Population-Level Immune Evasion and Affects Subtype Diversification. Cell Rep., 7(2):448-463, 24 Apr 2014. PubMed ID: 24726370. Show all entries for this paper.