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Displaying record number 55658

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location
Epitope	KIRWIIGLNK	Epitope Alignment
Epitope Name	KK10
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining, Proliferation, Tetramer binding
Keywords	TCR usage, characterizing CD8+ T cells

Notes

• By studying HIV-1 dysregulation of CTLs at different infection stages induced by inhibitory KIRs (Killer Immunoglobulin-like receptors), it was determined that KIR surface expression on memory T cells correlates with HIV replication. It results in reduced activation, proliferation, cytokine secretion, and killing following TCR stimulation. Since non-TCR-dependent CTL stimulation was unaffected, TCR-mediated stimulation appears to be defective. KIR induced suppression of CTL function was found to be KIR-ligand-independent.
• KK10-specific CTLs had heterogeneous surface expression of KIR. Of these tetramer positive B27-CTLs, only KIR- cells were able to secrete IFN-gamma upon stimulation. These KK10-specific, HLA-B27-restricted CTL were also used for an HIV inhibition assay.

References

Alter2008 Galit Alter, Suzannah Rihn, Hendrik Streeck, Nickolas Teigen, Alicja Piechocka-Trocha, Kristin Moss, Kristen Cohen, Angela Meier, Florencia Pereyra, Bruce Walker, and Marcus Altfeld. Ligand-Independent Exhaustion of Killer Immunoglobulin-Like Receptor-Positive CD8+ T Cells in Human Immunodeficiency Virus Type 1 Infection. J. Virol., 82(19):9668-9677, Oct 2008. PubMed ID: 18579582. Show all entries for this paper.