Search CTL/CD8+ T-Cell Epitope Database

Found 13 matching records:

Displaying record number 53099

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	p17(18-27)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Species (MHC/HLA)	human(A*03)
Immunogen	HIV-1 infection
Country	Spain
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Proliferation
Keywords	HAART, ART, supervised treatment interruptions (STI)

Notes

Structured treatment interruption (STI) alone is not able to control viral replication in chronically infected patients, probably due to lack of strong T-helper cell responses. Only patients starting with moderately high viral load (VL) were able to reduce the VL set point. HIV-1 specific CD8+ T-cell responses were shown to increase significantly until the end of the follow up.
8/14 patients recognized this epitope.

References

Plana2004 Montserrat Plana, Felipe Garcia, Annette Oxenius, Gabriel M. Ortiz, Anna Lopez, Anna Cruceta, Gabriel Mestre, Emilio Fumero, Catherine Fagard, Maria Antonia Sambeat, Ferran Segura, José M. Miró, Mireia Arnedo, L. Lopalcos, Tomas Pumarola, Bernard Hirschel, Rodney E. Phillips, Douglas F. Nixon, Teresa Gallart, and Jose M. Gatell. Relevance of HIV-1-Specific CD4+ Helper T-Cell Responses During Structured Treatment Interruptions in Patients With CD4+ T-Cell Nadir Above 400/mm3. J. Acquir. Immune Defic. Syndr., 36(3):791-799, 1 Jul 2004. PubMed ID: 15213562. Show all entries for this paper.

Displaying record number 58492

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag(18-27)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A03, A11, B07, B27)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	rate of progression, characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-A*03/A*11/B*07/B*27-restricted Gag optimal epitope KIRLRPGGKK was tested in a dual color EliSpot and found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Peretz2011 Yoav Peretz, Olivia Marra, Réjean Thomas, Danielle Legault, Pierre Côté, Mohamed-Rachid Boulassel, Danielle Rouleau, Jean-Pierre Routy, Rafick-Pierre Sékaly, Christos M Tsoukas, Cécile Tremblay, and Nicole F. Bernard. Relative Contribution of HIV-Specific Functional Lymphocyte Subsets Restricted by Protective and Non-Protective HLA Alleles. Viral Immunol, 24(3):189-198, Jun 2011. PubMed ID: 21668360. Show all entries for this paper.

Displaying record number 53325

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*03:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 54839

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag( Henan isolate)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

CTL responses were studied in treated and untreated donors from China, using Gag peptides from Henan isolate. All peptides were predicted to have A2 or A11 restriction (two of the most prominent alleles in Chinese population) and HLA restriction was further analyzed by CTL recognition in patients carrying corresponding allele. Among treated donors, HLA-A2 donors had higher CTL response than than HLA-A11 donors. Chronically infected untreated individuals had higher magnitude of CTL response than treated ones.

References

Gong2006 Xiaoyan Gong, Xien Gui, Yuxia Zhang, and Po Tien. Screening for CD8 Cytotoxic T Lymphocytes Specific for Gag of Human Immunodeficiency Virus Type 1 Subtype B' Henan Isolate from China and Identification of Novel Epitopes Restricted by the HLA-A2 and HLA-A11 Alleles. J. Gen. Virol., 87(Pt 1):151-158, Jan 2006. PubMed ID: 16361427. Show all entries for this paper.

Displaying record number 52872

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	1272
Subtype	multiple
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	T-cell Elispot
Keywords	binding affinity, computational epitope prediction, HLA cross-presentation, novel epitope

Notes

Epitopes defined using sequence parsing and matching algorithm Conservatrix, and epitope prediction tool EpiMatrix, were shown to be conserved in a broad range of HIV-1 sequences derived from different parts of the world. 31 novel highly conserved HIV-1 epitopes were found, among which four were recognized as promiscuous epitopes and five as MHC supertypes.
Estimated binding probability for KIRLRPGGKK: 36%. This epitope has been previously reported to be presented by A3, B27, B62, Bw62 and is an A11 binder, but was not confirmed as a CTL target in this study.

References

DeGroot2003 Anne S. De Groot, Bill Jesdale, William Martin, Caitlin Saint Aubin, Hakima Sbai, Andrew Bosma, Judy Lieberman, Gail Skowron, Fadi Mansourati, and Kenneth H. Mayer. Mapping Cross-Clade HIV-1 Vaccine Epitopes Using a Bioinformatics Approach. Vaccine, 21(27-30):4486-4504, 1 Oct 2003. PubMed ID: 14505932. Show all entries for this paper.

Displaying record number 62509

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Netherlands
Experimental methods
Keywords	review, escape

Notes

This review primarily focuses on the role of HLA alleles in controlling HIV immune response and/or disease progression but also describes the role of polymorphic host immunomodulatory genes in HIV susceptibility.
In the Netherlands, B*27-specific CTL escape mutations have been documented including R264G in Gag-p24 KK10 and E260D (not in KK10). [Cornelissen2009, PMID: 19487902]

References

Kaur2009 G. Kaur and N. Mehra. Genetic Determinants of HIV-1 Infection and Progression to AIDS: Immune Response Genes. Tissue Antigens, 74(5):373-385, Nov 2009. PubMed ID: 19765261. Show all entries for this paper.

Displaying record number 62486

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	review, immunodominance, variant cross-reactivity, protective allele

Notes

This review focuses on how different immune cells vary with disease progression in HIV-infected patients as well as with patient outcomes (a) long-term non progressors (LTNPs) which may be - elite controllers (ECs) or virus controllers, (b)typical progressors (TPs), and (c) rapid progressors (RPs). Effects of HLA and chemokine receptor genotypes are summarized in Table 1 while the role of HIV-1 epitope-specific T cells towards progression or resistance is in Table 2. bNAbs are not linked with LTNPs except for glycan-dependent NAbs. (see Table 3). Suppression of HIV infection via NK cells is given in Table 4.
Mutations in epitopes recognized by immunodominant, protective HLA alleles are driven by immune pressure and impair viral fitness, i.e., a decrease in viral replicative capacity in the presence of protective alleles like B*27. N271H in B*27-KK10 results in a decrease of infectivity by 40% compared to wild type Gag. Some LTNPs could rearrange TCR to increase affinity of B*27:05 for epitopes including KK10.
Not all carriers of B*27 are controllers as there are progressors carrying the protective allele too, with no difference in the number of KK10-specific CTL. Functional activity however as in peptide loading, perforin delivery and broader cross-recognition are better in controllers and this is a function of the dominant TCR clonotype. CD73+ CTL also distinguish ECs from progressors.

References

Li2018 Liuzhe Li, Yan Liu, and Miroslaw K. Gorny. Association of Diverse Genotypes and Phenotypes of Immune Cells and Immunoglobulins With the Course of HIV-1 Infection. Front. Immunol., 9:2735, 2018. PubMed ID: 30534128. Show all entries for this paper.

Displaying record number 55616

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Country	Netherlands
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	computational epitope prediction

Notes

Peptide prediction programs based on MHC binding, proteasomal cleavage and TAP transport were applied to select novel epitopes absent from the Los Alamos HIV database. 22 novel epitopes were tested by IFN-gamma ELISpot and 19 (11 HLA-B27 restricted, 8 HLA-B57 restricted) induced strong positive responses.
Magnitude of response generated was similar to that of previously identified epitopes, but the number of individuals responding was lower. This could be explained by less stringent HLA-typing of subjects and perhaps high sequence variability within these novel epitopes.
KK10(Gag-p17, 18-27), KIRLRPGGKK, is a known HLA-B27-restricted epitope used as a positive control for eliciting CTL IFN-gamma response.

References

Schellens2008 Ingrid M. M. Schellens, Can Kesmir, Frank Miedema, Debbie van Baarle, and José A. M. Borghans. An Unanticipated Lack of Consensus Cytotoxic T Lymphocyte Epitopes in HIV-1 Databases: The Contribution of Prediction Programs. AIDS, 22(1):33-37, 2 Jan 2008. PubMed ID: 18090389. Show all entries for this paper.

Displaying record number 9

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	p17(18-27)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

A study of p17 variation considering known p17 epitopes and individuals with known HLA types revealed that p17 evolution is influenced by immune pressure from CTLs.

References

Birk1998 M. Birk, A. Vahlne, A. Sonnerborg, and M. Sallberg. Nonsynonymous Mutations within the Human Immunodeficiency Virus Type 1 p17 Gene Are Clustered to Sequences Binding to the Host Human Leukocyte Antigen Class I Molecules. AIDS Res. Hum. Retroviruses, 14:241-248, 1998. PubMed ID: 9491914. Show all entries for this paper.

Displaying record number 8

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	p17(18-27 LAI)
Epitope	`KIRLRPGGKK`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen
Experimental methods
Keywords

Notes

D. Lewinsohn, pers. comm.

References

Brander1996 Christian Brander and Bruce Walker. The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. In The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. pages IV--50--IV--60. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 1996. URL: http://hiv-web.lanl.gov/immunology. Show all entries for this paper.

Displaying record number 53673

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	Gag
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods	Other
Keywords	rate of progression, escape

Notes

Three distinct HLA alleles known to alter the rate of AIDS progression were studied. B*57-mediated protection occurs early in infection and the protective effect of this allele subsides after CD4 cell count drops. In contrast, B*27 shows no protection against progression to CD4<200, but rather delays progression to an AIDS-defining illness after the CD4 counts have dropped. B*35-mediated rapid progression to AIDS is probably a function of early decline in CD4 counts.
KK10 escape occurs late and was shown to precede a sharp increase in viral load. The authors hypothesize the B27 benefit may arise due to enduring HLA restriction after escape from many other allotype responses has occurred.

References

Gao2005 Xiaojiang Gao, Arman Bashirova, Astrid K. N. Iversen, John Phair, James J. Goedert, Susan Buchbinder, Keith Hoots, David Vlahov, Marcus Altfeld, Stephen J. O'Brien, and Mary Carrington. AIDS Restriction HLA Allotypes Target Distinct Intervals of HIV-1 Pathogenesis. Nat. Med., 11(12):1290-1292, Dec 2005. PubMed ID: 16288280. Show all entries for this paper.

Displaying record number 57842

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location	p24
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Subtype	B
Species (MHC/HLA)	human(B27, B35)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, TCR binding
Keywords	rate of progression, TCR usage, optimal epitope

Notes

The study measured the functional avidities of HIV-specific CTL responses targeting 20 optimally defined CTL epitopes in a cohort of 44 HIV controllers and 68 non-controllers. Highest functional avidities were toward HLA-B-restricted epitopes in Gag. Avidities were higher in HIV controllers than non-controllers. Higher-avidity T-cell populations had narrower TCR repertoires.
KIRLRPGGKK was one of 20 optimal epitopes tested.
Responses to B27-KK10 and B57-KF11 showed higher functional avidities than other responses studied.
Promiscuously-presented epitope KK10 (B27, B35) exhibited higher functional avidity in the context of the HLA allele associated with better disease outcome (B27).
High-avidity CTL responses to KK10 in controllers were ssociated with narrow TCR repertoire.

References

Berger2011 Christoph T. Berger, Nicole Frahm, David A. Price, Beatriz Mothe, Musie Ghebremichael, Kari L. Hartman, Leah M. Henry, Jason M. Brenchley, Laura E. Ruff, Vanessa Venturi, Florencia Pereyra, John Sidney, Alessandro Sette, Daniel C. Douek, Bruce D. Walker, Daniel E. Kaufmann, and Christian Brander. High-Functional-Avidity Cytotoxic T Lymphocyte Responses to HLA-B-Restricted Gag-Derived Epitopes Associated with Relative HIV Control. J. Virol., 85(18):9334-9345, Sep 2011. PubMed ID: 21752903. Show all entries for this paper.

Displaying record number 57856

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HXB2 Location	Gag(18-27) p17(18-27) DNA(841..870)	Gag Epitope Map
Author Location
Epitope	`KIRLRPGGKK`	Epitope Alignment
Epitope Name	KK10
Species (MHC/HLA)	human(B*27:05)
Immunogen	HIV-1 infection
Country	United Kingdom
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	rate of progression, early treatment

Notes

103 epitope-specific CTL responses were compared between 19 long-term ART patients who began therapy at the time of seroconversion and 15 long-term non-progressors. ART initiated at the time of seroconversion was associated with immunological features resembling long-term non-progressors.
Within the group of epitopes tested, 4 "favorable" epitopes were among the most commonly recognized. 67% of B*2705-positive patients in both cohorts recognized KIRLRPGGKK. Long-term non-progressors recognized "favorable" epitopes more frequently than did long-term ART-treated patients.

References

Cellerai2011 Cristina Cellerai, Alexandre Harari, Hans Stauss, Sabine Yerly, Anna-Maria Geretti, Anne Carroll, Thynn Yee, Jonathan Ainsworth, Ian Williams, John Sweeney, Andrew Freedman, Margaret Johnson, Giuseppe Pantaleo, and Sabine Kinloch-de Loes. Early and Prolonged Antiretroviral Therapy Is Associated with an HIV-1-Specific T-Cell Profile Comparable to That of Long-Term Non-Progressors. PLoS One, 6(4):e18164, 2011. PubMed ID: 21483676. Show all entries for this paper.