Search CTL/CD8+ T-Cell Epitope Database

Found 11 matching records:

Displaying record number 59911

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag(202-210)
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	KA9
Species (MHC/HLA)	human(A*24)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KETINEEAA had a significantly positive association with HLA A*24; it was recognized by 3/28 A*24-positive subjects and 2/252 A*24-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59910

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag(202-210)
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	KA9
Species (MHC/HLA)	human(B*08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KETINEEAA had a significantly positive association with HLA B*08; it was recognized by 2/19 B*08-positive subjects and 0/233 B*08-negative subjects.

References

Displaying record number 59912

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag(202-210)
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	KA9
Species (MHC/HLA)	human(B35:02, B35:03, B*53:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KETINEEAA had a significantly positive association with HLA B*3502,B*3503,B*5301; it was recognized by 4/33 B*3502,B*3503,B*5301-positive subjects and 5/280 B*3502,B*3503,B*5301-negative subjects.

References

Displaying record number 55265

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	p24(70-78)
Epitope	`KETINEEAA`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	assay or method development, optimal epitope

Notes

HLA-A11-restricted CTL epitopes in 2 male Han Chinese subjects (one ART naive and one receiving HAART) were determined. A novel epitope was discovered and 5 other putative epitopes confirmed using 425 overlapping peptides spanning the entire HIV-1 clade B proteome. By employing intracellular cytokine staining, the authors developed a new method of mapping optimal epitopes and defining their HLA restriction efficiently. In spite of its being the most common HLA type in China, A*11-restricted epitopes were recognized less frequently than other epitopes, and A*11-restricted responses formed a mean of only 13% of the responses against HIV.
This epitope, KETINEEAA, was detected within overlapping peptideS GGHQAAMQMLKETINEEA, LKETINEEAAEWDRLHPV and AAEWDRLHPVHAGPIA.

References

Wang2007 Shaoyang Wang, Yongtao Sun, Song Zhai, Yan Zhuang, Shuguang Zhao, Whenzhen Kang, Xinhong Li, Dedong Huang, Xu G. Yu, Bruce D. Walker, and Marcus A. Altfeld. Identification of HLA-A11-Restricted HIV-1-Specific Cytotoxic T-Lymphocyte Epitopes in China. Curr. HIV Res., 5(1):119-128, Jan 2007. PubMed ID: 17266563. Show all entries for this paper.

Displaying record number 61194

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag
Epitope	`KETINEEAA`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human, mouse(B*40)
Immunogen	HIV-1 infection, vaccine
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA, modified vaccinia Ankara (MVA), adenovirus
Vaccine strain	B clade

Notes

Using 3 strategies, (1) in silico-designed multivalent-mosaic proteins for greater breadth, magnitude, cross-reactivity (2) highly conserved epitopes for loss of replicative fitness and oligofunctional inter-clade specific T-cell production (3) beneficial CTL epitopes associated with low viral load (VL) for broad immune response; a conserved bivalent-mosaic immunogen, tHIV-consvX, was designed and vectored by plasmid DNA, ChAdOx1, and MVA for testing in mice and humans. Strong preclinical immunogenicity was seen in mice and in 120 untreated, chronic HIV-1 patients, tHIVconsvX-specific CTLs correlated with high CD4+ T cell count but not VL.
Env was excluded from this second generation vaccine as its short, conserved stretch has no beneficial epitopes and while its epitopes are immunodominant, they are not associated with good control of viremia.
Highly conserved epitope KETINEEAA was included in tHIVconsvX. It was found in 50% of 4,300 sequences, with its variant, KdTINEEAA being found in 41% of sequences in the database. Since both epitopes are present in the vaccine, combined coverage is 91%.

References

Ondondo2016 Beatrice Ondondo, Hayato Murakoshi, Genevieve Clutton, Sultan Abdul-Jawad, Edmund G.-T. Wee, Hiroyuki Gatanaga, Shinichi Oka, Andrew J. McMichael, Masafumi Takiguchi, Bette Korber, and Tomáš Hanke. Novel Conserved-region T-cell Mosaic Vaccine With High Global HIV-1 Coverage Is Recognized by Protective Responses in Untreated Infection. Mol. Ther., 24(4):832-842, Apr 2016. PubMed ID: 26743582. Show all entries for this paper.

Displaying record number 59909

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag(202-210)
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	KA9
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KETINEEAA had a significantly positive association with HLA B*40; it was recognized by 9/28 B*40-positive subjects and 9/313 B*40-negative subjects.

References

Displaying record number 56031

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	p24
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	KA9(p24)
Subtype	B
Species (MHC/HLA)	human(B40)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Previously described HLA-B40-restricted epitope KETINEEAA elicited an immune response in Chinese HIV-1 positive subjects as part of peptides GHQAAMQMLKETINEEAA and LKETINEEAAEWDRLHPV.
5 of the 20 HLA-B40 carriers responded to KETINEEAA-containing peptide #27 with average magnitude of CTL response of 450 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 1959

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	p24(70-78)
Epitope	`KETINEEAA`	Epitope Alignment
Epitope Name	Gag-KA9, p24-KA9
Subtype	B
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	01RCH46: A02:01, A02:17, B08:01, B40:02, C03:03, C07:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	HAART, ART, novel epitope

Notes

This study monitored epitope responses in HIV-1 infected minority women living in the United States.
24 epitopes were described -- 8 were novel, 8 used new restricting elements but were previously defined epitopes, and 8 were previously described.
Serial peptide truncations were used to define optimal epitopes for CTL cell lines isolated from 12 individuals, assayed by a Cr-release.
This epitope was newly defined in this study.
Patient 01RCH46 was Hispanic, on HAART, and had a viral load of 21000 and CD4 count of 623 -- she also recognized GELDRWEKI, p17(11-19), HLA B*4002, and TAFTIPSI, RT(128-135), HLA A*0217.
Among HIV+ individuals who carried HLA B40, 3/5 (60%) recognized this epitope.

References

Sabbaj2003 Steffanie Sabbaj, Anju Bansal, G. Douglas Ritter, Christopher Perkins, Bradley H. Edwards, Ethan Gough, Jianming Tang, James J. Szinger, Bette Korber, Craig M. Wilson, Richard A. Kaslow, Mark J. Mulligan, and Paul A. Goepfert. Cross-Reactive CD8+ T Cell Epitopes Identified in US Adolescent Minorities. J. Acquir. Immune Defic. Syndr., 33(4):426-438, 1 Aug 2003. PubMed ID: 12869831. Show all entries for this paper.

Displaying record number 52569

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	p24(70-78)
Epitope	`KETINEEAA`	Epitope Alignment
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 58038

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	Gag
Epitope	`KETINEEAA`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	A24:02, A68:01, B15:03, B40:06, C02:02, C15:02
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine type	adenovirus type 5 (Ad5), DNA prime with Ad5 boost
Vaccine strain	B clade IIIB, B clade JRFL, B clade CAM-1
Vaccine component	Gag, Nef, Pol

Notes

T-cell epitope response specificities were examined in uninfected subjects who received the Gag/Pol/Nef vaccine used in the Step trial. Variable epitopes were detected more frequently than would be expected from an unbiased sampling. The most conserved epitopes were detected at a lower frequency, similar to the frequency reported for responses to natural infection, suggesting potential epitope masking. This may be a mechanism used by the virus in both contexts to escape effective T-cell surveillance. Development of vaccine strategies that can elicit a greater breadth, and towards conserved regions of the genome in particular, are critical requirements for effective T-cell based vaccines.
All vacinees had responses to the Gag peptide pool, and most also had responses to the Nef and/or Pol pools. Subsequent mapping using 9mer minipools demonstrated that T-cell epitopes were recognized across the length of all 3 proteins. A total of 105 distinct 9mers were detected, although some of these may have been overlapping 9mers representing a single epitope.
1 vaccinee recognized 9mer KETINEEAA.

References

Li2011a Fusheng Li, Adam C. Finnefrock, Sheri A. Dubey, Bette T. M. Korber, James Szinger, Suzanne Cole, M. Juliana McElrath, John W. Shiver, Danilo R. Casimiro, Lawrence Corey, and Steven G Self. Mapping HIV-1 Vaccine Induced T-Cell Responses: Bias Towards Less-Conserved Regions and Potential Impact on Vaccine Efficacy in the Step Study. PLoS One, 6(6):e20479, 2011. PubMed ID: 21695251. Show all entries for this paper.

Displaying record number 62799

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HXB2 Location	Gag(202-210) p24(70-78) DNA(1393..1419)	Gag Epitope Map
Author Location	p24(202-210)
Epitope	`KETINEEAA`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 48 single nucleotide mutations were observed in epitope KETINEEAA (known to bind to B4002) with a log transformation of mean fitness score, relative to NL4-3, of -0.7281.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.