Search CTL/CD8+ T-Cell Epitope Database

Found 13 matching records:

Displaying record number 59883

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KAAVDLSHFL had a significantly positive association with HLA A*11; it was recognized by 4/18 A*11-positive subjects and 8/145 A*11-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59884

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Species (MHC/HLA)	human(B*57)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KAAVDLSHFL had a significantly positive association with HLA B*57; it was recognized by 15/88 B*57-positive subjects and 12/163 B*57-negative subjects.

References

Displaying record number 59882

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Species (MHC/HLA)	human(C*03)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KAAVDLSHFL had a significantly positive association with HLA C*03; it was recognized by 20/49 C*03-positive subjects and 27/251 C*03-negative subjects.

References

Displaying record number 59881

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Species (MHC/HLA)	human(C*08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
KAAVDLSHFL had a significantly positive association with HLA C*08; it was recognized by 29/41 C*08-positive subjects and 47/300 C*08-negative subjects.

References

Displaying record number 62253

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91 HXB2)
Epitope	`KAAVDLSHFL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(C*08)
Immunogen	HIV-1 infection
Patient MHC/HLA	15160: A03, A32, B14, B44
Country	United States
Experimental methods	Chromium-release assay
Keywords	autologous responses, novel epitope

Notes

C. Brander notes that this is an A*3201 epitope in the 1999 database.
A long term non-progressor (LTNP) was studied for detailed analyses of CTL response to epitopes correlated to the subject's viral quasispecies. Epitope mapping revealed CTL response to 6 epitopes in p17, p24, RT, Env and Nef. Results show that (a) multiple epitopes were targeted simultaneously (b) viral heterogeneity/antigenic diversity is not necessary for such a multispecific CTL response (c) LTNP infection does not need to be associated with unique epitope targeting as 5/6 epitopes targeted have been seen in other subjects with disease progression (d) HIV-1-specific CTL can be highly active, disallowing viral escapes, but broadly cross-reactive (recognizing known variants) even with low viral load (VL).
A Nef-specific clone, CA3, from the LTNP subject 15160 recognized a Nef, HLA-C*08-restricted novel epitope, KAAVDLSHFL, which overlaps other previously identified epitopes.

References

Harrer1996b T. Harrer, E. Harrer, S. A. Kalams, P. Barbosa, A. Trocha, R. P. Johnson, T. Elbeik, M. B. Feinberg, S. P. Buchbinder, and B. D. Walker. Cytotoxic T Lymphocytes in Asymptomatic Long-Term Nonprogressing HIV-1 Infection. Breadth and Specificity of the Response and Relation to In Vivo Viral Quasispecies in a Person with Prolonged Infection and Low Viral Load. J. Immunol., 156:2616-2623, 1996. PubMed ID: 8786327. Show all entries for this paper.

Displaying record number 1000

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91 LAI)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(C*08:02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

A patient who made a mono-specific CTL response to this Nef specific epitope was given effective anti-retroviral therapy within 90 days of infection, reducing the antigenic stimulus.
Within 7 days of therapy, his CTLp frequency dropped from 60 to 4 per million PBMC, as his viremia dropped.
The patient went from having an activated effector population (detected by CTLp and clone specific RNA) to a non-activated quiescent population (detected by the CTL-clone specific DNA)

References

Nixon1999 D. F. Nixon, D. Douek, P. J. Kuebler, X. Jin, M. Vesanen, S. Bonhoeffer, Y. Cao, R. A. Koup, D. D. Ho, and M. Markowitz. Molecular Tracking of an Human Immunodeficiency Virus Nef Specific Cytotoxic T Cell Clone Shows Persistence of Clone-Specific T Cell Receptor DNA but not mRNA Following Early Combination Antiretroviral Therapy. Immunol. Lett., 66:219-228, 1999. PubMed ID: 10203058. Show all entries for this paper.

Displaying record number 55049

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location
Epitope	`KAAVDLSHFL`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope KAAVDLSHFL elicited a magnitude of response of 540 SFC with a functional avidity of 0.01nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 55203

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location
Epitope	`KAAVDLSHFL`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	supertype, HLA cross-presentation

Notes

100 HIV-infected subjects were tested for response to optimally-defined CTL epitopes regardless of the subject's HLA type. Half of all detected responses were seen in the absence of the originally reported restricting HLA class I allele, and only 3% of epitopes were recognized exclusively in the presence of their original allele. Statistical and experimental methods were used to define additional HLA alleles associated with the epitopes.
In addition to its known HLA association (Cw08), an additional HLA (Cw03) was statistically predicted to be associated with this epitope.

References

Frahm2007b Nicole Frahm, Karina Yusim, Todd J. Suscovich, Sharon Adams, John Sidney, Peter Hraber, Hannah S. Hewitt, Caitlyn H. Linde, Daniel G. Kavanagh, Tonia Woodberry, Leah M. Henry, Kellie Faircloth, Jennifer Listgarten, Carl Kadie, Nebojsa Jojic, Kaori Sango, Nancy V. Brown, Eunice Pae, M. Tauheed Zaman, Florian Bihl, Ashok Khatri, Mina John, Simon Mallal, Francesco M. Marincola, Bruce D . Walker, Alessandro Sette, David Heckerman, Bette T. Korber, and Christian Brander. Extensive HLA Class I Allele Promiscuity among Viral CTL Epitopes. Eur. J. Immunol., 37(9):2419-2433, Sep 2007. PubMed ID: 17705138. Show all entries for this paper.

Displaying record number 54112

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Species (MHC/HLA)	human(Cw8)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a Cw*08 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53539

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Subtype	B
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A28, A29, B14, B44, Cw8
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, escape, characterizing CD8+ T cells, viral fitness and reversion

Notes

Two-thirds of all mutations arising in non-envelope proteins during the transition from acute to chronic infection were found to be due to either CD8 T-cell-associated selective pressures or reversion of HLA-I-associated mutations selected in a previous host.The predominance of escape mutations at highly polymorphic sites was observed across different HIV-1 clades.
One escape mutation, at position 6, KAAVDmSHFL, was found not to correspond to the most polymorphic residue in the epitope.

References

Allen2005a Todd M. Allen, Marcus Altfeld, Shaun C. Geer, Elizabeth T. Kalife, Corey Moore, Kristin M. O'Sullivan, Ivna DeSouza, Margaret E. Feeney, Robert L. Eldridge, Erica L. Maier, Daniel E. Kaufmann, Matthew P. Lahaie, Laura Reyor, Giancarlo Tanzi, Mary N. Johnston, Christian Brander, Rika Draenert, Jurgen K. Rockstroh, Heiko Jessen, Eric S. Rosenberg, Simon A. Mallal, and Bruce D. Walker. Selective Escape from CD8+ T-Cell Responses Represents a Major Driving Force of Human Immunodeficiency Virus Type 1 (HIV-1) Sequence Diversity and Reveals Constraints on HIV-1 Evolution. J. Virol., 79(21):13239-13249, Nov 2005. PubMed ID: 16227247. Show all entries for this paper.

Displaying record number 53377

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	( B consensus)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Epitope Name	KL10
Subtype	B
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Patient MHC/HLA	A25, A32, B14, B8, Cw7, Cw8
Country	United States
Experimental methods	Chromium-release assay, Cytokine production, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	assay or method development, memory cells, characterizing CD8+ T cells

Notes

Using a flow-cytometric cytotoxicity assay based on caspase-3 activation in dying target cells, it was shown that the subset of HIV-1-specific CD8+ T cells secreting both IFN-gamma and TNF-alpha exhibit stronger cytotoxic activity than those secreting only IFN-gamma. These cells also exhibited stronger intracellular perforin expression. No association between HIV-1-specific CD8+ T-cell maturation phenotypes and intracellular perforin expression was found.
1/9 individuals recognized this epitope.

References

Lichterfeld2004b Mathias Lichterfeld, Xu G. Yu, Michael T. Waring, Stanley K. Mui, Mary N. Johnston, Daniel Cohen, Marylyn M. Addo, John Zaunders, Galit Alter, Eunice Pae, Daryld Strick, Todd M. Allen, Eric S. Rosenberg, Bruce D. Walker, and Marcus Altfeld. HIV-1-Specific Cytotoxicity Is Preferentially Mediated by a Subset of CD8+ T Cells Producing both Interferon-gamma and Tumor Necrosis Factor-alpha. Blood, 104(2):487-394, 15 Jul 2004. PubMed ID: 15059848. Show all entries for this paper.

Displaying record number 1848

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef( SF2)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

This epitope was mapped by ELISPOT in a study identifying new HLA-B60 epitopes, and was one of the epitopes presented by another HLA molecule in an HLA-B60 individual.

References

Altfeld2000 M. A. Altfeld, A. Trocha, R. L. Eldridge, E. S. Rosenberg, M. N. Phillips, M. M. Addo, R. P. Sekaly, S. A. Kalams, S. A. Burchett, K. McIntosh, B. D. Walker, and P. J. Goulder. Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay. J. Virol., 74:8541, 2000. PubMed ID: 10954555. Show all entries for this paper.

Displaying record number 1549

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HXB2 Location	Nef(82-91) DNA(9040..9069)	Nef Epitope Map
Author Location	Nef(82-91 SF2)
Epitope	`KAAVDLSHFL`	Epitope Alignment
Species (MHC/HLA)	human(Cw8)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, acute/early infection

Notes

Therapy provided during acute infection resulted in a narrower CTL response, stronger T help response, and a less diverse viral population than was seen in individuals treated during chronic infection.
The breadth and specificity of the response was determined using ELISPOT by studying 19 individuals with pre-seroconversion therapy (Group 1), 11 individuals with primary infection but post-seroconversion therapy (Group 2), and 10 individuals who responded to HAART given during chronic infection (Group 3), using 259 overlapping peptides spanning p17, p24, RT, gp41, gp120 and Nef.
Previously described and newly defined optimal epitopes were tested for CTL response.
Number of HLA-Cw8+ individuals that had a CTL response to this epitope broken down by group: 1/3 group 1, 0/0 group 2, and 0/1 group 3.

References

Altfeld2001b M. Altfeld, E. S. Rosenberg, R. Shankarappa, J. S. Mukherjee, F. M. Hecht, R. L. Eldridge, M. M. Addo, S. H. Poon, M. N. Phillips, G. K. Robbins, P. E. Sax, S. Boswell, J. O. Kahn, C. Brander, P. J. Goulder, J. A. Levy, J. I. Mullins, and B. D. Walker. Cellular immune responses and viral diversity in individuals treated during acute and early HIV-1 infection. J. Exp. Med., 193(2):169-80, 15 Jan 2001. URL: http://www.jem.org/cgi/content/full/193/2/169. PubMed ID: 11148221. Show all entries for this paper.