Search CTL/CD8+ T-Cell Epitope Database

Found 20 matching records:

Displaying record number 59855

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(A*01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA A*01; it was recognized by 4/29 A*01-positive subjects and 3/106 A*01-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59853

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA A*11; it was recognized by 3/10 A*11-positive subjects and 9/141 A*11-negative subjects.

References

Displaying record number 59857

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(A*25)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA A*25; it was recognized by 2/6 A*25-positive subjects and 7/135 A*25-negative subjects.

References

Displaying record number 59848

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(A*31)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA A*31; it was recognized by 11/19 A*31-positive subjects and 60/269 A*31-negative subjects.

References

Displaying record number 54278

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	A68-IR11(Tat)
Species (MHC/HLA)	human(A68)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contribution of immune responses restricted to individual HLA class I molecules were studied.
The most frequently recognised epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contribution of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 52415

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(38-48)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A68)
Immunogen	HIV-1 infection
Experimental methods
Keywords	early-expressed proteins

Notes

CTL responses against regulatory and accessory proteins in 70 HIV-1 infected patients were studied using overlapping peptides and Elispot.
28% targeted one or more overlapping Tat peptides; 36%, Rev peptides; 33%, Vif peptides; 40%, Vpr peptides; and 2%, Vpu peptides.
All known optimally defined epitopes were summarized for the five proteins.

References

Addo2002a M. M. Addo, X. G. Yu, E. S. Rosenberg, B. D. Walker, and M. Altfeld. Cytotoxic T-Lymphocyte (CTL) Responses Directed against Regulatory and Accessory Proteins in HIV-1 Infection. DNA Cell Biol., 21(9):671-678, Sep 2002. PubMed ID: 12396610. Show all entries for this paper.

Displaying record number 57284

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Subtype	B
Species (MHC/HLA)	human(A*68)
Immunogen	HIV-1 infection
Country	Barbados, Haiti, Jamaica
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	mother-to-infant transmission, elite controllers

Notes

To dissect whether the protective effect of HLA-B*57 in HIV-1 infection is due to CTL that recognize HLA-B*57-restricted epitopes or by the presence of viral-fitness attenuating mutations in such B*57 epitopes, 13 mother-infant pairs were studied. The B57 advantage is primarily through the maintenance of a fitness-attenuating mutation in p24-Gag, T242N in epitope TW10 (TSTLQEQIGW).
Regardless of parental inheritance, HLA-B57 controls VL even in infancy and even when escape mutations from B*57 positive mothers are transmitted. Mutations in p24-Gag are predominant in mismatched-HLA subject pairs and arise after vertical transmission, impacting viral fitness.
Non-B57-epitope IR11-Tat, ITKGLGISYGR restricted by A*68, was recognized by an infant with maternally-inherited B57.

References

Schneidewind2009 Arne Schneidewind, Yanhua Tang, Mark A. Brockman, Elizabeth G. Ryland, Jacqueline Dunkley-Thompson, Julianne C. Steel-Duncan, M. Anne St. John, Joseph A. Conrad, Spyros A. Kalams, Francine Noel, Todd M. Allen, Celia D. Christie, and Margaret E. Feeney. Maternal Transmission of Human Immunodeficiency Virus Escape Mutations Subverts HLA-B57 Immunodominance but Facilitates Viral Control in the Haploidentical Infant. J. Virol., 83(17):8616-8627, Sep 2009. PubMed ID: 19515764. Show all entries for this paper.

Displaying record number 54864

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*6801)
Immunogen	peptide-HLA interaction
Experimental methods	Cytokine production, Flow cytometric T-cell cytokine assay
Keywords	binding affinity, co-receptor, characterizing CD8+ T cells

Notes

It was found that CD8 co-receptor differentially fine tunes CTL function via cytokine/chemokine production (MCP-1, MIP1-beta, MIP1-alpha, TNF-alpha, RANTES, IFN-gamma, IL-2 and IL-4). Differential CD8 action was controlled by abrogating its engagement using point-mutated HLA Class I molecules in 4 CTL clones specific for 3 different epitopes from HIV-1 and hTERT.
An HLA-A*6801 restricted CTL clone, c23, specific for HIV-1 Tat epitope ITKGLGISYGR found to require a much higher peptide concentration to activate effector functions. This clone did not secrete IL-4.

References

Laugel2007a Bruno Laugel, David A. Price, Anita Milicic, and Andrew K. Sewell. CD8 Exerts Differential Effects on the Deployment of Cytotoxic T Lymphocyte Effector Functions. Eur. J. Immunol., 37(4):905-913, Apr 2007. PubMed ID: 17393387. Show all entries for this paper.

Displaying record number 52173

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(38-48)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	Tat-4.8
Species (MHC/HLA)	human(A*6801)
Immunogen	HIV-1 infection
Experimental methods
Keywords	assay or method development

Notes

This epitope and HLA-A*6801 presenting molecule were rapidly defined using a modified Elispot assay.
The 11-mer is the optimal epitope but A*6801 epitopes tolerate length variation.

References

Oxenius2002a Annette Oxenius, Bent K. Jakobsen, Philippa J. Easterbrook, Jonathan M. Boulter, Tin Tun, Anele Waters, Juliet Agudelo, Martin Barnardo, Rodney E. Phillips, and David A. Price. Complete Mapping of a Novel HLA A*6801-Restricted HIV-1 Tat Epitope Directly with a Rapid Modified Enzyme-Linked Immunospot Assay. AIDS, 16(9):1285-1287, 14 Jun 2002. PubMed ID: 12045495. Show all entries for this paper.

Displaying record number 53418

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(38-48)
Epitope	`ITKGLGISYGR`	Epitope Alignment
		Show epitope variants
Epitope Name	ITK
Species (MHC/HLA)	human(A*6801)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2, A68.1, B07, B3503, Cw0401, Cw0702, DQ2, DQ6, DR15, DR17, DR51, DR52
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, escape

Notes

The increase in plasma viral load in a patient that progressed rapidly was preceded by positive selection of viral escape mutations in epitopes targeted by dominant HIV-1-specific CD8+ T-cell responses,and a decrease in HIV-1-specific CD4+ and CD8+ T-cell frequencies. Overall, escape variant epitopes were recognized 0-80% as efficiently as the index peptide, and the relatively efficiency of the variant epitopes increased using PBLs collected after their appearance. No changes were found in viral tropism, replication kinetics and neutralizing antibody titers, so the rapid decline of the patient was attributed to loss of HIV containment due to CTL escape.
This was one of 8 reactive epitopes found not to vary over time.

References

Oxenius2004 Annette Oxenius, David A. Price, Alexandra Trkola, Charles Edwards, Emma Gostick, Hua-Tang Zhang, Philippa J. Easterbrook, Tin Tun, Andrew Johnson, Anele Waters, Edward C. Holmes, and Rodney E. Phillips. Loss of Viral Control in Early HIV-1 Infection Is Temporally Associated with Sequential Escape from CD8+ T Cell Responses and Decrease in HIV-1-Specific CD4+ and CD8+ T Cell Frequencies. J. Infect. Dis., 190(4):713-721, 15 Aug 2004. PubMed ID: 15272399. Show all entries for this paper.

Displaying record number 52546

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Species (MHC/HLA)	human(A*6801)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 54589

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(38-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Species (MHC/HLA)	human(A*6801)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Degranulation, CD107a and b cell surface mobilization, Other
Keywords	binding affinity, epitope processing, kinetics, characterizing CD8+ T cells

Notes

HLA-A*6801 was studied because of its unusual property of weak binding to CD8. Several peptide variants were followed in a CTL clone grown from an A*68 expressing patient who responded to his autologous "index" peptide iTKgLGISYGR. His founder epitope was suggested to be TTKALGISYGR, the "reference" sequence, with variants ITKaLGISYGR and tTKGLGISYGR. The reference sequence TTKALGISYGR is a better agonist in cytokine and degranulation assays than the index peptide iTKgLGISYGR. Surface plasmon resonance shows that both index and reference peptides bind soluble TCR within normal ranges. A mutant HLA-A68 that increases CD8 interaction was found to recognize CTL at lower peptide concentrations and increase cytokine production (IFN-g, MIP-1b, RANTES). However its pattern of variant recognition was not changed. Thus "normalization" of HLA-A*6801 interaction with CD8 does not induce non-specific activation, but does enhance agonist peptide recognition and immune response.

References

Gostick2007 Emma Gostick, David K. Cole, Sarah L. Hutchinson, Linda Wooldridge, Sabrina Tafuro, Bruno Laugel, Anna Lissina, Annette Oxenius, Jonathan M. Boulter, David A. Price, and Andrew K. Sewell. Functional and Biophysical Characterization of an HLA-A*6801-Restricted HIV-Specific T Cell Receptor. Eur. J. Immunol., 37(2):479-486, Feb 2007. PubMed ID: 17273992. Show all entries for this paper.

Displaying record number 59851

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*07; it was recognized by 18/36 B*07-positive subjects and 71/288 B*07-negative subjects.

References

Displaying record number 59849

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B*1501)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*1501; it was recognized by 12/24 B*1501-positive subjects and 48/245 B*1501-negative subjects.

References

Displaying record number 59847

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*1503; it was recognized by 6/10 B*1503-positive subjects and 26/194 B*1503-negative subjects.

References

Displaying record number 59854

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B3502, B3503, B*5301)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*3502,B*3503,B*5301; it was recognized by 6/24 B*3502,B*3503,B*5301-positive subjects and 15/160 B*3502,B*3503,B*5301-negative subjects.

References

Displaying record number 59856

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B*49)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*49; it was recognized by 3/9 B*49-positive subjects and 12/151 B*49-negative subjects.

References

Displaying record number 59852

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(B*81)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA B*81; it was recognized by 5/10 B*81-positive subjects and 21/184 B*81-negative subjects.

References

Displaying record number 59850

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA C*12; it was recognized by 16/41 C*12-positive subjects and 32/204 C*12-negative subjects.

References

Displaying record number 59846

HXB2 Location	Tat(39-49) DNA(5945..5977)	Tat Epitope Map
Author Location	Tat(39-49)
Epitope	`ITKGLGISYGR`	Epitope Alignment
Epitope Name	IR11
Species (MHC/HLA)	human(C*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
ITKGLGISYGR had a significantly positive association with HLA C*14; it was recognized by 13/17 C*14-positive subjects and 89/324 C*14-negative subjects.