Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 59015

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HXB2 Location	Gag(147-158) p24(15-26) DNA(1228..1263)	Gag Epitope Map
Author Location	Gag(147-158)
Epitope	`ISPRTLNAWVKV`	Epitope Alignment
		Show epitope variants
Epitope Name	CE1
Subtype	B, C, M group
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine type	DNA
Vaccine strain	M group consensus
Vaccine component	Gag

Notes

Immune response to universally conserved Gag epitopes was increased by using a p24CE plasmid immunogen vaccine. 7, 12-24 amino acid CE (conserved elements) were put together separated by 2-4 residue spacers, into 2 different DNA immunogens. The immunogens differed by one-amino acid in each of the CE. Mice were vaccinated and later challenged with Gag peptide pools from subtypes -A, -B and -C. This method of vaccine construction eliminates immunodominant decoy sequences that suppress expression of and response to sub-dominant epitope sequences. Multifunctional, robust responses of both CD4+ and CD8+ T cells were seen to 4/7 CE in the p24CE vaccine as opposed to only 2/7 poor, helper T cell anti-CE responses when immunized with the p55^gag control.
Conserved Element 1, ISPRTLNAWVKV, was one of the CE used in the p24CE1 vaccine, as representing the HXB2, and Clades B-, C- and M-consensus. Clade-A variant, lSPRTLNAWVKV, was used in the p24CE2 vaccine. This epitope containing peptide, CE1, elicited a strong helper T cell response to vaccinated-mice splenocytes as opposed to a weaker response when mock vaccinated.

References

Kulkarni2013 Viraj Kulkarni, Margherita Rosati, Antonio Valentin, Brunda Ganneru, Ashish K. Singh, Jian Yan, Morgane Rolland, Candido Alicea, Rachel Kelly Beach, Gen-Mu Zhang, Sylvie Le Gall, Kate E. Broderick, Niranjan Y. Sardesai, David Heckerman, Beatriz Mothe, Christian Brander, David B. Weiner, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. HIV-1 p24(gag) Derived Conserved Element DNA Vaccine Increases the Breadth of Immune Response in Mice. PLoS One, 8(3):e60245, 2013. PubMed ID: 23555935. Show all entries for this paper.

Displaying record number 59227

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HXB2 Location	Gag(147-158) p24(15-26) DNA(1228..1263)	Gag Epitope Map
Author Location	Gag
Epitope	`ISPRTLNAWVKV`	Epitope Alignment
Epitope Name	CE1
Subtype	M group
Species (MHC/HLA)	macaque
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	DNA
Vaccine strain	M group consensus
Vaccine component	Gag

Notes

Immunogenicity of p24CE plasmid DNA vaccine containing 7 Gag conserved elements was studied in macaques. A novel strategy involving vaccine prime followed by a p55^gag DNA boost increases magnitude and breadth of CTL immune response to Gag as well as alters hierarchy of epitope recognition, avoiding decoy epitopes.
ISPRTLNAWVKV Group M peptide (CE1) did not induce CTL response in 10 macaques 2 weeks after priming with vaccine-containing p24CE1 (ISPRTLNAWVKV) and p24CE2, (lSPRTLNAWVKV) followed by Gag boost.

References

Kulkarni2014 Viraj Kulkarni, Antonio Valentin, Margherita Rosati, Candido Alicea, Ashish K. Singh, Rashmi Jalah, Kate E. Broderick, Niranjan Y. Sardesai, Sylvie Le Gall, Beatriz Mothe, Christian Brander, Morgane Rolland, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. Altered Response Hierarchy and Increased T-Cell Breadth upon HIV-1 Conserved Element DNA Vaccination in Macaques. PLoS One, 9(1):e86254, 2014. PubMed ID: 24465991. Show all entries for this paper.

Displaying record number 61914

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HXB2 Location	Gag(147-158) p24(15-26) DNA(1228..1263)	Gag Epitope Map
Author Location	Gag(439-474)
Epitope	`ISPRTLNAWVKV`	Epitope Alignment
Epitope Name	CE1-1
Species (MHC/HLA)	human, macaque
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, vaccine antigen design, SIV

Notes

To circumvent immunodominant, variable responses to vaccines, a conserved element (CE) SIV p27gag plasmid DNA (pDNA) vaccine was generated by homology to a previous HIV p24gag pDNA vaccine. The p27gag pDNA vaccine was successful in developing robust subdominant CTL responses targeting CE in the vaccine as analyzed in 31 rhesus macaques. Breadth of recognition and response was widened by novel booster regimens - either gag pDNA or codelivery of p27CE+gag pDNA, both increasing the number of CE-specific CTLs. The CE+gag pDNA booster, however, elicited significantly broader recognition and thus changed the immunodominance hierarchy and profile.
Conserved elements CE1 comprised ISPRTLNAWVKV as well as CE1-2 LSPRTLNAWVKV in order to cover >98% of known sequences from HIV-1M group as part of the HIV Gagp24 CE. In SIV, this corresponded to p27CE1, LSPRTLNAWVKL.

References

Hu2016 Xintao Hu, Antonio Valentin, Frances Dayton, Viraj Kulkarni, Candido Alicea, Margherita Rosati, Bhabadeb Chowdhury, Rajeev Gautam, Kate E. Broderick, Niranjan Y. Sardesai, Malcolm A. Martin, James I. Mullins, George N. Pavlakis, and Barbara K. Felber. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J. Immunol., 197(10):3999-4013, 15 Nov 2016. PubMed ID: 27733554. Show all entries for this paper.