Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 55244

HXB2 Location	Pol(918-926) Integrase(203-211) DNA(4836..4862)	Pol Epitope Map
Author Location	Integrase(202-212)
Epitope	IIATDIQTK	Epitope Alignment
Epitope Name	IK9
Subtype	B
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	assay or method development, optimal epitope

Notes

• HLA-A11-restricted CTL epitopes in 2 male Han Chinese subjects (one ART naive and one receiving HAART) were determined. A novel epitope was discovered and 5 other putative epitopes confirmed using 425 overlapping peptides spanning the entire HIV-1 clade B proteome. By employing intracellular cytokine staining, the authors developed a new method of mapping optimal epitopes and defining their HLA restriction efficiently. In spite of its being the most common HLA type in China, A*11-restricted epitopes were recognized less frequently than other epitopes, and A*11-restricted responses formed a mean of only 13% of the responses against HIV.
• This novel epitope, IIATDIQTK (IK9), was detected and confirmed within overlapping peptides SAGERIVDIIATDIQTK and DIIATDIQTKELQKQITK.

References

Wang2007 Shaoyang Wang, Yongtao Sun, Song Zhai, Yan Zhuang, Shuguang Zhao, Whenzhen Kang, Xinhong Li, Dedong Huang, Xu G. Yu, Bruce D. Walker, and Marcus A. Altfeld. Identification of HLA-A11-Restricted HIV-1-Specific Cytotoxic T-Lymphocyte Epitopes in China. Curr. HIV Res., 5(1):119-128, Jan 2007. PubMed ID: 17266563. Show all entries for this paper.

Displaying record number 58608

HXB2 Location	Pol(918-926) Integrase(203-211) DNA(4836..4862)	Pol Epitope Map
Author Location	Integrase(202-212)
Epitope	IIATDIQTK	Epitope Alignment
Epitope Name	IK9
Subtype	B
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection
Country	China
Experimental methods
Keywords	optimal epitope

Notes

• C. Brander noted this as an optimal A*11 epitope based on data from <cite>Wang 2007</cite>.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61874

HXB2 Location	Pol(918-926) Integrase(203-211) DNA(4836..4862)	Pol Epitope Map
Author Location
Epitope	IIATDIQTK	Epitope Alignment
Epitope Name	IK9
Subtype	B
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection, vaccine
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses

Notes

• Specific T cells - from 200 ART-naive, infected Japanese subjects previously exposed to the tHIVconsvX vaccine - that have the ability to suppress HIV-1 replication were identified as recognizing 6 Pol epitopes. 6/25 (comprising 20 known and 5 novel mapped) epitopes remain associated with positive clinical outcome. The candidate tHIVconsvX vaccine is composed of 6 conserved Gag and Pol regions.
• A*11-restricted Pol epitope IIATDIQTK was found in 15-mer peptide pair C362/363. 1 HLA-carrying responders and 10 non-HLA responders to the peptides were detected by EliSpot out of 20 subjects, while 1/221 Japanese subjects responded to the epitope.

References

Zou2019 Chengcheng Zou, Hayato Murakoshi, Nozomi Kuse, Tomohiro Akahoshi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, Tomáš Hanke, and Masafumi Takiguchi. Effective Suppression of HIV-1 Replication by Cytotoxic T Lymphocytes Specific for Pol Epitopes in Conserved Mosaic Vaccine Immunogens. J. Virol., 93(7), 1 Apr 2019. PubMed ID: 30674626. Show all entries for this paper.