Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 53950

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HXB2 Location	Rev(52-60) DNA(8456..8482)	Rev Epitope Map
Author Location	( C consensus)
Epitope	IHSISERIL	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

• A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
• Mutational patterns in the H2 residue of IHSISERIL are associated with the presence of the HLA presenting molecule in the host.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 56244

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HXB2 Location	Rev(52-60) DNA(8456..8482)	Rev Epitope Map
Author Location
Epitope	IHSISERIL	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*23:01, A*29:02, B*15:10, B*45:01, C*06:02, C*16:01; A*23:01, B*08:01, B*15:10, C*07:01, C*16:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression

Notes

• 47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
• Known epitope IHSISERIL, is HLA-B*1510-restricted. Response to a peptide containing this epitope was detected in an early controller and a rapid progressor 12 weeks post-infection.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 62227

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HXB2 Location	Rev(52-60) DNA(8456..8482)	Rev Epitope Map
Author Location	Rev(52-60)
Epitope	IHSISERIL	Epitope Alignment
		Show epitope variants
Epitope Name	Rev-IL9
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Patient MHC/HLA	517-C: A*30:02, A*68:02, B*15:10, B*42:01, C*03:04, C*17:01; 517-M: A*30:02, A*30:04, B*42:01, B*58:02, C*06:02, C*17:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	dynamics, responses in children, mother-to-infant transmission, rate of progression, escape

Notes

• 11 perinatally infected pediatric slow progressors (PSPs) were followed longitudinally for a decade from birth to examine CTL responses to circulating and autologous HIV. It was found by ultra-deep sequencing that though, like adults, children develop variants early in infection; unlike adults, pediatric anti-variant CTL are generated.
• EliSpot was used to determine that non-Gag/Pol-specific responses (i.e. Env or Nef responses) are higher within the first weeks of infection for pediatric viremic non-controllers (VNCs) just as seen previously in adult VNCs and vice versa in pediatric VCs (PSPs). Therefore non-Gag/Pol-specific CTL responses are confirmed to be linked to higher VL and progression to disease.
• In pediatric VNC, 517-C, B*1510-restricted IF9 was transmitted as WT from the B*1510^- mother (Patient Rec. #4459) to the B*1510⁺ child. Variants were seen to arise over the time course, but they are not identified escape variants.

References

Leitman2017 Ellen M. Leitman, Christina F. Thobakgale, Emily Adland, M. Azim Ansari, Jayna Raghwani, Andrew J. Prendergast, Gareth Tudor-Williams, Photini Kiepiela, Joris Hemelaar, Jacqui Brener, Ming-Han Tsai, Masahiko Mori, Lynn Riddell, Graz Luzzi, Pieter Jooste, Thumbi Ndung'u, Bruce D. Walker, Oliver G. Pybus, Paul Kellam, Vivek Naranbhai, Philippa C. Matthews, Astrid Gall, and Philip J. R. Goulder. Role of HIV-Specific CD8+ T Cells in Pediatric HIV Cure Strategies after Widespread Early Viral Escape. J. Exp. Med., 214(11):3239-3261, 6 Nov 2017. PubMed ID: 28983013. Show all entries for this paper.

Displaying record number 58949

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HXB2 Location	Rev(52-60) DNA(8456..8482)	Rev Epitope Map
Author Location	Rev(52-60)
Epitope	IHSISERIL	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP045: A*23:01, A*29:02, B*15:10, B*45:01, C*16:01, C*16:02
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	escape, acute/early infection

Notes

• Amino acid mutations in HIV-1 subtype C were tracked within the first 6 months following transmission in 5 subjects. Early, rapid adaptations (55 mutations) to the viral genome were predominantly driven by CTL pressure (38%), but also by antibody pressure (35%) and reversions (16%). Most CTL escape occurred in Nef, followed by Pol and Env.
• Epitope IHSISERIL underwent mutations at amino acids H, position 2 and L, position 9. These changes were first seen 5-12 weeks after transmission. The escapes were tracked in subject CAP45.
• Using autologous peptides, 13/21 putative epitopes were confirmed to be responsive [see Liu et al. J. Clinical Investigation. 123:380-93 (2013) for details]

References

Abrahams2013 Melissa-Rose Abrahams, Florette K. Treurnicht, Nobubelo K. Ngandu, Sarah A. Goodier, Jinny C. Marais, Helba Bredell, Ruwayhida Thebus, Debra de Assis Rosa, Koleka Mlisana, Cathal Seoighe, Salim Abdool Karim, Clive M. Gray, and Carolyn Williamson. Rapid, Complex Adaptation of Transmitted HIV-1 Full-Length Genomes in Subtype C-Infected Individuals with Differing Disease Progression. AIDS, 27(4):507-518, 20 Feb 2013. PubMed ID: 23370465. Show all entries for this paper.

Displaying record number 61039

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HXB2 Location	Rev(52-60) DNA(8456..8482)	Rev Epitope Map
Author Location	Rev(52-60)
Epitope	IHSISERIL	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP045: A*23:01, A*29:02, B*15:10, B*45:01, C*16:01, C*16:02
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

• 17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
• Epitope IHSISERIL had a fitness cost ΔE of 3.195 and T cell response was first detected to it at 21d, escape time was 191.09 days.

References

Barton2016 John P. Barton, Nilu Goonetilleke, Thomas C. Butler, Bruce D. Walker, Andrew J. McMichael, and Arup K. Chakraborty. Relative Rate and Location of Intra-Host HIV Evolution to Evade Cellular Immunity Are Predictable. Nat. Commun., 7:11660, 23 May 2016. PubMed ID: 27212475. Show all entries for this paper.