Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 52775

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HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location	( 89.6)
Epitope	`IGPGRAFYAR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2D^d)
Immunogen	vaccine
Experimental methods	Intracellular cytokine staining
Keywords	vaccine-induced immune responses, dendritic cells

Vaccine Details

Vaccine type	DNA
Vaccine component	gp120
Adjuvant	Flex, a dendric cell growth factor

Notes

BALB/c mice were given a DNA vaccine that contained gp120 DNA covalently attached to the extracellular domain of the Fms-like tyrosine kinase receptor-3 ligand (FLex), a dendritic cell growth factor.
Mice vaccinated intramuscularly with the FLex:gp120 chimeric gene gave a dendritic cell (DC) expansion similar to native Flex protein.
gp120-specific stable CD8+ T-cell responses lasted 114 days after a prime/boost, and were observed in the presence and absence of Flex-DNA-induced DC expansion; strong Ab responses required DC expansion.

References

Sailaja2003 Gangadhara Sailaja, Sajid Husain, Bishnu P. Nayak, and Abdul M. Jabbar. Long-Term Maintenance of gp120-Specific Immune Responses by Genetic Vaccination with the HIV-1 Envelope Genes Linked to the Gene Encoding Flt-3 Ligand. J. Immunol., 170(5):2496-507, Mar 2003. PubMed ID: 12594275. Show all entries for this paper.

Displaying record number 55740

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HXB2 Location	gp160(311-320) gp120(311-320) DNA(7155..7184)	gp160 Epitope Map
Author Location
Epitope	`IGPGRAFYAR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	vaccine
Experimental methods	Chromium-release assay, Intracellular cytokine staining, Other
Keywords	vaccine antigen design, co-receptor

Vaccine Details

Vaccine type	modified vaccinia Ankara (MVA)
Vaccine strain	B clade 89.6
Vaccine component	Env

Notes

While propagating MVA encoding HIV 89.6 Env, excessively staining foci were studied and found to possess a single nucleotide deletion that conferred upon Env a 115 aa C-terminal truncation. Truncated Env was more highly expressed and so induced higher antibody and CTL responses without compromising its ability for CD4/co-receptor fusion. A similar truncation would be beneficial in MVA-based vaccines.
To test for CTL response to Env epitopes, peptide P18-89.6R10, IGPGRAFYAR, was used as stimulating peptide in Chromium release assays where wild type MVA/89.6 and truncated MVA/89.6T induced similar specific lytic activities. Intracellular staining also showed similar induction of IFN-γ+; IFN-γ+, TNF+; and IFN-γ+, IL-2+ CTLs by both forms of the virus.

References

Wyatt2008a Linda S. Wyatt, Igor M. Belyakov, Patricia L. Earl, Jay A. Berzofsky, and Bernard Moss. Enhanced Cell Surface Expression, Immunogenicity and Genetic Stability Resulting from a Spontaneous Truncation of HIV Env Expressed by a Recombinant MVA. Virology, 372(2):260-272, 15 Mar 2008. PubMed ID: 18048074. Show all entries for this paper.