Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 58172

HXB2 Location	Pol(799-808) Integrase(84-93) DNA(4479..4508)	Pol Epitope Map
Author Location	Pol(799-808 NL-432)
Epitope	`IEAEVIPAET`	Epitope Alignment
Epitope Name	IT10
Subtype	B
Species (MHC/HLA)	human(B*4002)
Immunogen	HIV-1 infection
Patient MHC/HLA	A0207, A3101, B4002, B4601, Cw0102, Cw0304
Country	Japan
Experimental methods	Intracellular cytokine staining
Keywords	optimal epitope, novel epitope

Notes

Four novel HLA-B*4002-restricted Integrase (Pol) epitopes were discovered using 11-mer overlapping peptides from Pol, Gag and Nef to elicit CTL responses from an HIV-1-infected subject. Optimal epitopes were identified using truncated peptides.
Within 11-mer IG11, IEAEVIPAETG (Pol 799-809), HLA-B*4002-restricted Integrase epitope IT10, IEAEVIPAET, was confirmed as being the optimal epitope.

References

Watanabe2011 Tamayo Watanabe, Hayato Murakoshi, Hiroyuki Gatanaga, Madoka Koyanagi, Shinichi Oka, and Masafumi Takiguchi. Effective Recognition of HIV-1-Infected Cells by HIV-1 Integrase-Specific HLA-B*4002-Restricted T Cells. Microbes Infect., 13(2):160-166, Feb 2011. PubMed ID: 20971209. Show all entries for this paper.

Displaying record number 58622

HXB2 Location	Pol(799-808) Integrase(84-93) DNA(4479..4508)	Pol Epitope Map
Author Location	Pol(799-808 NL-432)
Epitope	`IEAEVIPAET`	Epitope Alignment
Epitope Name	IT10
Subtype	B
Species (MHC/HLA)	human(B*4002)
Immunogen	HIV-1 infection
Patient MHC/HLA	B*4002
Country	Japan
Experimental methods
Keywords	optimal epitope

Notes

C. Brander noted this as an optimal B*4002 epitope based on data from <cite>Watanabe2011</cite>.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61516

HXB2 Location	Pol(799-808) Integrase(84-93) DNA(4479..4508)	Pol Epitope Map
Author Location	Gag(799-808)
Epitope	`IEAEVIPAET`	Epitope Alignment
Epitope Name	GagIT10
Subtype	B
Species (MHC/HLA)	human(B*4006)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	optimal epitope, novel epitope, chronic infection

Notes

CTL controlling HIV-1 and variants were identified from 401 subtype B chronically-infected, treatment-naive Japanese patients. 7 novel epitopes were identified (paper reports 9, correction made via author communication). 9/13 CTL-specific epitopes were conserved among the above subjects and 3/13 were cross-recognized by CTL. 5/13 epitopes were B*5201, 3/13 were B*6701-restricted, both HLA were found to play a predominant role in control of HIV-1. This is the first study to identify HIV-1-specific T cells clinically controlling HIV-1 in a population in whom protective HLA-B*57 and HLA-B*27 are very rare.
Breadth of response of all 13 epitope-specific CTL had synergistic effects on control, directly correlating to CD4 cell count and inversely to plasma viral load (pVL).
Novel epitope, HLA-B*4006-restricted PolIT10, IEAEVIPAET, had 25/393 responding subjects and did significantly correlate with pVL or CD4 count, suggesting B*4006 as a protective allele. IT10 is a conserved epitope.

References

Murakoshi2015 Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Takuya Naruto, Rie Maruyama, Yoshiko Tamura, Naoki Ishizuka, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Clinical Control of HIV-1 by Cytotoxic T Cells Specific for Multiple Conserved Epitopes. J. Virol., 89(10):5330-5339, May 2015. PubMed ID: 25741000. Show all entries for this paper.

Displaying record number 61886

HXB2 Location	Pol(799-808) Integrase(84-93) DNA(4479..4508)	Pol Epitope Map
Author Location
Epitope	`IEAEVIPAET`	Epitope Alignment
		Show epitope variants
Epitope Name	Pol IT10
Subtype	B
Species (MHC/HLA)	human(B*4006)
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	KI-928: ; KI-808: B*4006; KI-620: ; KI-878:
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses, viral replication

Notes

CTL specific for 10 epitopes that are associated with good clinical outcomes (Murakoshi2018, Zou2019) were shown to have strong ability to suppress HIV-1 replication. These CTL could recognize HIV-1-infected cells and most circulating HIV-1 in Japan as the 5 HLA-alleles restricting these 10 epitopes are represented in >50% of the Japanese population surveyed.
B*4006-restricted protective epitope, IEAEVIPAET, Pol IT10 was found in 85.3% chronically infected Japanese individuals and 72.4% of them who were B*4006+. Variants of this WT epitope were found too, mEAEVIPAET, IT10-1M, lEAEVIPAET, IT10-IL and IEAEVIsAET, IT10-7S. All variants except for IT10-7S were cross-recognized by IT10-specific CTL.

References

Murakoshi2019 Hayato Murakoshi, Nozomi Kuse, Tomohiro Akahoshi, Yu Zhang, Takayuki Chikata, Mohamed Ali Borghan, Hiroyuki Gatanaga, Shinichi Oka, Keiko Sakai, and Masafumi Takiguchi. Broad Recognition of Circulating HIV-1 by HIV-1-Specific Cytotoxic T-Lymphocytes with Strong Ability to Suppress HIV-1 Replication. J. Virol., 93(1), 1 Jan 2019. PubMed ID: 30333175. Show all entries for this paper.