Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 1743

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HXB2 Location	Gag(82-91) p17(82-91) DNA(1033..1062)	Gag Epitope Map
Author Location	p17(82-91 93TH253 subtype CRF01)
Epitope	`IATLWCVHQR`	Epitope Alignment
Subtype	CRF01_AE
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection
Experimental methods
Keywords	subtype comparisons

Notes

HLA-A*11 CRF01 (called subtype E in Bond et al.) epitopes were identified that stimulated CTL from HIV+ female sex workers (FSW) from Northern Thailand, of whom more than half were HLA-A*11 positive.
77 possible HLA-A*11 epitopes were first defined using EpiMatrix, these were screened for binding to A*11 finding and 26 bound, and 12 of these were epitopes for CTL responses from 8 HLA-A*11 positive FSWs, six were novel, six were previously identified.
This epitope was predicted by the EpiMatrix method to be likely to bind to A*11, and it served as an epitope in the FSWs, it was one of the six A*11 epitopes that had been previously defined.
3/8 tested FSWs recognized this epitope.
This epitope was not conserved in other subtypes, and exact matches were uncommon.

References

Bond2001 K. B. Bond, B. Sriwanthana, T. W. Hodge, A. S. De Groot, T. D. Mastro, N. L. Young, N. Promadej, J. D. Altman, K. Limpakarnjanarat, and J. M. McNicholl. An HLA-directed molecular and bioinformatics approach identifies new HLA-A11 HIV-1 subtype E cytotoxic T lymphocyte epitopes in HIV-1-infected Thais. AIDS Res. Hum. Retroviruses, 17(8):703-17, 20 May 2001. PubMed ID: 11429111. Show all entries for this paper.

Displaying record number 1723

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HXB2 Location	Gag(82-91) p17(82-91) DNA(1033..1062)	Gag Epitope Map
Author Location	p17(82-91 93TH253 subtype CRF01)
Epitope	`IATLWCVHQR`	Epitope Alignment
Epitope Name	G82-91
Subtype	CRF01_AE
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

This was a study of HIV-1 exposed persistently seronegative (HEPS) female sex workers in Chiang Mai, northern Thailand.
HLA-A11 is very common in this population, and was enriched among the HEPS sexworkers -- weak CTL responses were detected in 4/7 HEPS women, and CTL responses were found in 8/8 HIV+ controls, and 0/9 HIV- women that were not exposed.
This epitope was weakly reactive in the HEPS study subject 265 who was HLA A2/A11.
This epitope was strongly reactive in HIV+ study subject 053 who carried HLA-A11.

References

Sriwanthana2001 B. Sriwanthana, T. Hodge, T. D. Mastro, C. S. Dezzutti, K. Bond, H. A. Stephens, L. G. Kostrikis, K. Limpakarnjanarat, N. L. Young, S. H. Qari, R. B. Lal, D. Chandanayingyong, and J. M. McNicholl. HIV-specific cytotoxic T lymphocytes, HLA-A11, and chemokine-related factors may act synergistically to determine HIV resistance in CCR5 delta32-negative female sex workers in Chiang Rai, northern Thailand. AIDS Res. Hum. Retroviruses, 17(8):719-34, 20 May 2001. PubMed ID: 11429112. Show all entries for this paper.

Displaying record number 61336

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HXB2 Location	Gag(82-91) p17(82-91) DNA(1033..1062)	Gag Epitope Map
Author Location	Gag
Epitope	`IATLWCVHQR`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	vaccine
Country	Australia, Brazil, Canada, Dominican Republic, Haiti, Jamaica, Peru, Puerto Rico, United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, assay or method development, optimal epitope

Vaccine Details

Vaccine type	adenovirus type 5 (Ad5)
Vaccine component	Gag, Nef, Pol

Notes

Small pools of 15-mer peptides (7-30) were used to map T-cell epitopes. 2 such methods (mini- and maxi-pool strategies) used up to 7.7x less EliSpot assays than the traditional 'test-all' method of using each peptide-epitope individually. While the test-all method identified the largest number of epitopes and some EliSpots are only positive when shorter, more optimal peptides are used, overall this strategy is best for following vaccine trials and other large epitope-mapping studies.
37 15-mers and 27 optimal peptides representing 13 optimal epitopes gave CTL responses out of the 134 tested.
Optimal epitope IATLWCVHQR elicited response of 328.3 sfc/million PBMC from participants in the Step HIV-1 vaccine efficacy trial who were uninfected at the time of testing.

References

Fiore-Gartland2016 Andrew Fiore-Gartland, Bryce A. Manso, David P. Friedrich, Erin E. Gabriel, Greg Finak, Zoe Moodie, Tomer Hertz, Stephen C. De Rosa, Nicole Frahm, Peter B. Gilbert, and M Juliana McElrath. Pooled-Peptide Epitope Mapping Strategies Are Efficient and Highly Sensitive: An Evaluation of Methods for Identifying Human T Cell Epitope Specificities in Large-Scale HIV Vaccine Efficacy Trials. PLoS One, 11(2):e0147812, 2016. PubMed ID: 26863315. Show all entries for this paper.