Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 55677

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	Gag
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*23:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity

Notes

Transmission of HIV-1-escape variants from individuals with protective HLA-B*57/-B*5801 alleles to those without these alleles was studied to determine if infection with such replication-deficient mutants offered any advantage to the new host. Dependency between slower rate of disease progression in the newly infected subject and protective genotype of the infecting individual was suggested.
HLA-A*2301-restricted epitope HYMLKHLVW, within peptide GKKHYMLKHLVWASREL was able to elicit CTL response in 2 wild type virus-carrying subjects.

References

Chopera2008 Denis R. Chopera, Zenda Woodman, Koleka Mlisana, Mandla Mlotshwa, Darren P. Martin, Cathal Seoighe, Florette Treurnicht, Debra Assis de Rosa, Winston Hide, Salim Abdool Karim, Clive M. Gray, Carolyn Williamson, and CAPRISA 002 Study Team. Transmission of HIV-1 CTL Escape Variants Provides HLA-Mismatched Recipients with a Survival Advantage. PLoS Pathog, 4(3):e1000033, Mar 2008. PubMed ID: 18369479. Show all entries for this paper.

Displaying record number 57694

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	p17
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*23:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	A23:01, A23:01, B08:01, B15:10, C07:01, C16:01; A23:01, A29:02, B42:02, B44:03, C17:01, C17:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, acute/early infection

Notes

Immunological responses in early subtype C infection were characterized. Nef, Pol and Gag were the most frequently targeted proteins from as early as 3 to 8 weeks postinfection. Nef was immunodominant, with more than 75% of individuals recognizing Nef. Many responses to peptide pools were derived from the recognition of one immunodominant peptide.
Two A*2301-positive acutely HIV-1-infected individuals had a peptide response to presumed optimal epitope HYMLKHLVW within peptide GKKHYMLKHLVWASREL. For one patient, autologous viral escape occurred in 11.5 weeks, and response was lost at 30 weeks. For the other patient, there was no evidence of escape.

References

Mlotshwa2010 Mandla Mlotshwa, Catherine Riou, Denis Chopera, Debra de Assis Rosa, Roman Ntale, Florette Treunicht, Zenda Woodman, Lise Werner, Francois van Loggerenberg, Koleka Mlisana, Salim Abdool Karim, Carolyn Williamson, Clive M Gray, and CAPRISA 002 Study Team. Fluidity of HIV-1-Specific T-Cell Responses during Acute and Early Subtype C HIV-1 Infection and Associations with Early Disease Progression. J. Virol., 84(22):12018-12029, Nov 2010. PubMed ID: 20826686. Show all entries for this paper.

Displaying record number 53278

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A23:01, A24:02)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HLA cross-presentation, characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 56245

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A23:01, A24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A23:01, B08:01, B15:10, C07:01, C*16:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression

Notes

47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
Known epitope HYMLKHLVW is HLA-A*2301 and -A*2402-restricted. Response to a peptide containing this epitope was detected in a rapid progressor 12 weeks post-infection.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 54174

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	(28-36)
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Experimental methods	Other
Keywords	HLA associated polymorphism

Notes

Publicly available HIV-1 subtype C sequences from South Africa and Botswana were analyzed, and 94 HLA-associated amino acid polymorphisms were found. Many polymorphisms were associated with multiple HLA allele classes. 12% associations were negative: association of virus expression of the consensus amino acid with a specific HLA allele. HLA-B alleles accounted for 50% of associations. 19 HLA-associated polymorphisms were embedded in previously defined epitopes.
HYMLKHLVW was a previously defined A*2402 presented epitope that encompassed an A*24 associated polymorphism, HYmLKHLVW, in the third position.

References

Boutwell2007 Christian L. Boutwell and M. Essex. Identification of HLA Class I-Associated Amino Acid Polymorphisms in the HIV-1C Proteome. AIDS Res. Hum. Retroviruses, 23(1):165-174, Jan 2007. PubMed ID: 17263647. Show all entries for this paper.

Displaying record number 55475

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HXB2 Location	Gag(28-36) p17(28-36) DNA(871..897)	Gag Epitope Map
Author Location	Gag
Epitope	`HYMLKHLVW`	Epitope Alignment
Subtype	A1, B, C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, variant cross-reactivity, vaccine antigen design

Notes

From HIV-1 sequences of multiple subtypes, bioinformatic prediction generated 174 CTL epitopes from Gag, Pol, Nef, Env and Tat restricted by HLA supertypes A1, A2, A3, A24, B7, B44 and B58; 10 previously-published A2- and B58-restricted epitopes were also included. Of these 184 epitopes, 113 were recognized experimentally in serum from 31 HIV+ patients.
While Gag, Pol, Env and Nef were highly immunogenic, only 38% of Tat epitopes elicited a response.
HYMLKHLVW was a predicted epitope restricted by A24 supertype. 4/31 patient(s) responded to it; 1 responding patient was known to carry an allele of A24 supertype. Responding patients were infected with HIV clade(s) A1, B, C.
One of 21 epitopes denoted as elite, having a response in 4 or more of the 31 patients.
Broadly immunogenic epitope HYMLKHLVW, had subtype variants that were recognized by less than half its patient responders. HLA supertype restriction was predicted to be supertype A24.

References

Perez2008 Carina L. Pérez, Mette V. Larsen, Rasmus Gustafsson, Melissa M. Norström, Ann Atlas, Douglas F. Nixon, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Broadly Immunogenic HLA Class I Supertype-Restricted Elite CTL Epitopes Recognized in a Diverse Population Infected with Different HIV-1 Subtypes. J. Immunol., 180(7):5092-5100, 1 Apr 2008. PubMed ID: 18354235. Show all entries for this paper.