Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 54835

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HXB2 Location	Gag(194-202) p24(62-70) DNA(1369..1395)	Gag Epitope Map
Author Location	Gag( Henan isolate)
Epitope	HQAAMQMLK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A11)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

• CTL responses were studied in treated and untreated donors from China, using Gag peptides from Henan isolate. All peptides were predicted to have A2 or A11 restriction (two of the most prominent alleles in Chinese population) and HLA restriction was further analyzed by CTL recognition in patients carrying corresponding allele. Among treated donors, HLA-A2 donors had higher CTL response than than HLA-A11 donors. Chronically infected untreated individuals had higher magnitude of CTL response than treated ones.

References

Gong2006 Xiaoyan Gong, Xien Gui, Yuxia Zhang, and Po Tien. Screening for CD8 Cytotoxic T Lymphocytes Specific for Gag of Human Immunodeficiency Virus Type 1 Subtype B' Henan Isolate from China and Identification of Novel Epitopes Restricted by the HLA-A2 and HLA-A11 Alleles. J. Gen. Virol., 87(Pt 1):151-158, Jan 2006. PubMed ID: 16361427. Show all entries for this paper.

Displaying record number 205

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HXB2 Location	Gag(194-202) p24(62-70) DNA(1369..1395)	Gag Epitope Map
Author Location	p24(194-202 LAI)
Epitope	HQAAMQMLK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B52)
Immunogen
Experimental methods
Keywords

Notes

• P. Goulder, pers. comm.

References

Brander1996 Christian Brander and Bruce Walker. The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. In The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. pages IV--50--IV--60. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 1996. URL: http://hiv-web.lanl.gov/immunology. Show all entries for this paper.

Displaying record number 56447

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HXB2 Location	Gag(194-202) p24(62-70) DNA(1369..1395)	Gag Epitope Map
Author Location	p24
Epitope	HQAAMQMLK	Epitope Alignment
Epitope Name	HK9(p24)
Subtype	B
Species (MHC/HLA)	human(B52)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	chronic infection

Notes

• 49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
• An inverse correlation was found between CTL response and viral load.
• Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
• Although the tested peptide sequence contains the exact sequence of a previously described HLA-B52 optimal epitope, HQAAMQMLK, none of the 5 HLA-B52 carriers responded to it (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 58457

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HXB2 Location	Gag(194-202) p24(62-70) DNA(1369..1395)	Gag Epitope Map
Author Location	p24(62-70)
Epitope	HQAAMQMLK	Epitope Alignment
Subtype	A1
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Proliferation
Keywords	assay or method development, characterizing CD8+ T cells

Notes

• 24 longitudinally studied HIV-1 (clades A1 or D)-chronically-infected Kenyan subjects were tested for CTL response to 9-mer overlapping p24 peptides. Epitope mapping was assessed not just by IFN-γ EliSpot assays, but by multiparametric flow cytometry of intracellular cytokine staining for IL-2, tumor necrosis factor (TNF-α), chemokine macrophage inflammatory protein 1β (MIP-1β) and degranulation marker CD107a, as well as by proliferation assays. 50/69 epitope-specific responses detected were IFN-γ negative. Several epitope responses had polyfunctional (33%) and proliferative (19%) components. Long term non progressors (LTNP) had more polyfunctional responses of greater magnitude and proliferation than normal progressors (NP).
• 16 subjects were tested specifically with 126 putative epitopes, 50 of which were confirmed. Clade A1 epitope HQAAMQMLK, corresponds to B consensus of the same sequence, and elicited a single TNF-α response to one NP; and a single IFN-γ response to an LTNP.

References

Richmond2011 Meika Richmond, Lyle R. McKinnon, Sandra A. Koesters Kiazyk, Charles Wachihi, Makobu Kimani, Joshua Kimani, Francis A. Plummer, and T. Blake Ball. Epitope Mapping of HIV-Specific CD8+ T Cell Responses by Multiple Immunological Readouts Reveals Distinct Specificities Defined by Function. J. Virol., 85(3):1275-1286, Feb 2011. PubMed ID: 21084478. Show all entries for this paper.