Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 62232

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	Vif(48-57)
Epitope	`HPKVSSEVHI`	Epitope Alignment
		Show epitope variants
Epitope Name	Vif-HI10
Subtype	C
Species (MHC/HLA)	human(B*42)
Immunogen	HIV-1 infection
Patient MHC/HLA	517-C: A30:02, A68:02, B15:10, B42:01, C03:04, C17:01; 517-M: A30:02, A30:04, B42:01, B58:02, C06:02, C17:01; 021-C: A02:05, A29:02, B42:01, B44:03, C02:10, C17:01; 021-M: A02:05, A33:01, B42:01, B44:03, C02:10, C17:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	dynamics, responses in children, mother-to-infant transmission, rate of progression, escape

Notes

11 perinatally infected pediatric slow progressors (PSPs) were followed longitudinally for a decade from birth to examine CTL responses to circulating and autologous HIV. It was found by ultra-deep sequencing that though, like adults, children develop variants early in infection; unlike adults, pediatric anti-variant CTL are generated.
EliSpot was used to determine that non-Gag/Pol-specific responses (i.e. Env or Nef responses) are higher within the first weeks of infection for pediatric viremic non-controllers (VNCs) just as seen previously in adult VNCs and vice versa in pediatric VCs (PSPs). Therefore non-Gag/Pol-specific CTL responses are confirmed to be linked to higher VL and progression to disease.
In pediatric VNCs 517-C and 021-C, B*42-restricted HI10 was transmitted with mutations not documented as escapes from the B*42⁺ mothers (Patient Rec. #4459 and #4461) to the B*42⁺ children. Variants were seen to arise over the time course, but they are not identified escape variants.

References

Leitman2017 Ellen M. Leitman, Christina F. Thobakgale, Emily Adland, M. Azim Ansari, Jayna Raghwani, Andrew J. Prendergast, Gareth Tudor-Williams, Photini Kiepiela, Joris Hemelaar, Jacqui Brener, Ming-Han Tsai, Masahiko Mori, Lynn Riddell, Graz Luzzi, Pieter Jooste, Thumbi Ndung'u, Bruce D. Walker, Oliver G. Pybus, Paul Kellam, Vivek Naranbhai, Philippa C. Matthews, Astrid Gall, and Philip J. R. Goulder. Role of HIV-Specific CD8+ T Cells in Pediatric HIV Cure Strategies after Widespread Early Viral Escape. J. Exp. Med., 214(11):3239-3261, 6 Nov 2017. PubMed ID: 28983013. Show all entries for this paper.

Displaying record number 53951

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	( C consensus)
Epitope	`HPKVSSEVHI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
Mutational patterns in the H1 residue of HPKVSSEVHI are associated with the presence of the HLA presenting molecule in the host.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 53304

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	( C consensus)
Epitope	`HPKVSSEVHI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 59160

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	Vif(22-30)
Epitope	`HPKVSSEVHI`	Epitope Alignment
Epitope Name	HI10-Vif
Subtype	C
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Tetramer binding
Keywords	TCR usage, characterizing CD8+ T cells

Notes

Inhibitory receptor [programmed death-1 (PD-1)/lymphocyte activation gene-3 (LAG-3)/ T-cell immunoglobulin and mucin domain-3 (Tim-3)] expression was studied during chronic infection in 128 CTL populations from 32 ART-naive, sub-Saharan, HIV-clade C-infected subjects expressing either HLA-B*1503 or -B*4201. PD-1 was found to vary across epitope specificity whether inter- or intra-subject. PD-1 also varied with different TCR clonotypes even within one HIV-specific CTL population. PD-1 correlates directly with viral load (VL).
CTL specific for HI10-Vif, HPKVSSEVHI, a highly variable epitope, were found to lack significant correlation with VL.

References

Kloverpris2014a Henrik N. Kløverpris, Reuben McGregor, James E McLaren, Kristin Ladell, Anette Stryhn, Catherine Koofhethile, Jacqui Brener, Fabian Chen, Lynn Riddell, Luzzi Graziano, Paul Klenerman, Alasdair Leslie, Søren Buus, David A. Price, and Philip Goulder. Programmed Death-1 Expression on HIV-1-Specific CD8+ T Cells Is Shaped by Epitope Specificity, T-Cell Receptor Clonotype Usage and Antigen Load. AIDS, 28(14):2007-2021, 10 Sep 2014. PubMed ID: 24906112. Show all entries for this paper.

Displaying record number 59439

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	Vif(48-57)
Epitope	`HPKVSSEVHI`	Epitope Alignment
		Show epitope variants
Epitope Name	Vif-HI10
Subtype	C
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Country	Botswana, South Africa, United Kingdom
Experimental methods	CD8 T-cell Elispot - IFNγ, Other, Tetramer binding
Keywords	immunodominance, escape, optimal epitope, novel epitope

Notes

2,093 HLA-B*4201⁺ ART-naive female adults from 5 cohorts with chronic C-clade infection were studied to determine factors controlling immunodominance. Immunodominant hierarchy of response observed across 20 HLA-B*4201-restricted epitopes was not linked to peptide-B*4201 binding affinity or stability. Immunodominance correlated instead with the particular amino acid residues within (TCRβ) TRB sequences. Extreme TCR biases towards generating identical or 'public' epitope-specific TCRs in multiple individuals therefore were statistically the greatest influence upon Immunodomination.
13 B*4201⁺-restricted novel epitopes were identified by EliSpot screening of 181 females with 410 HIV-1 OLPs.
11 different public clonotypes were identified and were used by the top 3 ranked immunodominant epitope-specificities.
6/10 most immunodominant epitopes were Gag/Pol/Vpr-specific and 100% showed selection pressure on HIV. Of 4 epitopes that were Nef/Vif-specific and among most immunodominant, only 25% i.e one epitope experienced selection pressure in the virus.
Novel epitope HPKVSSEVHI from OLP Vif-407 (ESRHPKVSSEVHIPLG) had a targeting frequency of ˜32% of 154 HLA-B*4201⁺ subjects. Immunodominance rank was 6/20 tested epitopes. Vif-HI10-specific responses trend with higher median viral loads (VL).
Vif-HI10-HLA-B*4202's binding affinity, K_d = 38 nM; stability, K_1/2 = 17.1h.
1 polymorphism was selected by HLA-B*4201 in this Vif-HI10 epitope, H48N.

References

Kloverpris2015 Henrik N Kløverpris, Reuben McGregor, James E. McLaren, Kristin Ladell, Mikkel Harndahl, Anette Stryhn, Jonathan M. Carlson, Catherine Koofhethile, Bram Gerritsen, Can Keşmir, Fabian Chen, Lynn Riddell, Graz Luzzi, Alasdair Leslie, Bruce D. Walker, Thumbi Ndung'u, Søren Buus, David A. Price, and Philip J. Goulder. CD8+ TCR Bias and Immunodominance in HIV-1 Infection. J. Immunol., 194(11):5329-5345, 1 Jun 2015. PubMed ID: 25911754. Show all entries for this paper.

Displaying record number 56257

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location
Epitope	`HPKVSSEVHI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	A23:01, A29:02, B41:01, B42:01, C*17:01
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression

Notes

47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
Known epitope HPKVSSEVHI is HLA-B*4201-restricted. Response to a peptide containing this epitope was detected in 2 rapid progressors 12 weeks post-infection.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 54201

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HXB2 Location	Vif(48-57) DNA(5182..5211)	Vif Epitope Map
Author Location	Vif(48-57)
Epitope	`HPKVSSEVHI`	Epitope Alignment
Epitope Name	HI10
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other, Tetramer binding
Keywords	supertype, HLA cross-presentation, TCR usage, HLA associated polymorphism

Notes

14 promiscuous HIV-specific CTL epitopes presented by B7 supertype alleles were described. It was shown that CTLs targeting an identical epitope that are restricted by closely related but different HLA alelles differ in several ways: by occurrence of distinct HLA-specific escape mutations, by recruitment of distinct TCRs by peptide/MHC complexes, and by functional avidity of CTLs. Since there were no differences in the frequency, magnitude or immunodominance of the CTL responses restricted by different HLA alleles, it is suggested that the unique peptide/MHC complexes generated by closely related HLA induce qualitatively different CTL responses.
Statistically significant associations between numbers of HLA-4201 expressing subjects and epitope HPKVSSEVHI were found.
Functional avidity is correlated with selection pressure observed in HLA allele-epitope HI10 restriction

References

Leslie2006 Alasdair Leslie, David A. Price, Pamela Mkhize, Karen Bishop, Almas Rathod, Cheryl Day, Hayley Crawford, Isobella Honeyborne, Tedi E. Asher, Graz Luzzi, Anne Edwards, Christine M. Rousseau, James I. Mullins, Gareth Tudor-Williams, Vas Novelli, Christian Brander, Daniel C. Douek, Photini Kiepiela, Bruce D. Walker, and Philip J. R. Goulder. Differential Selection Pressure Exerted on HIV by CTL Targeting Identical Epitopes but Restricted by Distinct HLA Alleles from the Same HLA Supertype. J. Immunol., 177(7):4699-4708, 1 Oct 2006. PubMed ID: 16982909. Show all entries for this paper.