Search CTL/CD8+ T-Cell Epitope Database

Found 9 matching records:

Displaying record number 59758

HXB2 Location	Vif(28-36) DNA(5122..5148)	Vif Epitope Map
Author Location	Vif(28-36)
Epitope	`HMYISKKAK`	Epitope Alignment
Epitope Name	HK9
Species (MHC/HLA)	human(A*03)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
HMYISKKAK had a significantly positive association with HLA A*03; it was recognized by 4/60 A*03-positive subjects and 1/255 A*03-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 52529

HXB2 Location	Vif(28-36) DNA(5122..5148)	Vif Epitope Map
Author Location	Vif(28-36)
Epitope	`HMYISKKAK`	Epitope Alignment
Species (MHC/HLA)	human(A*0301)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61688

HXB2 Location	Vif(28-36) DNA(5122..5148)	Vif Epitope Map
Author Location	Vif(28-36)
Epitope	`HMYISKKAK`	Epitope Alignment
Epitope Name	HK9
Species (MHC/HLA)	human(A*0301)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	review

Notes

This review makes the argument that T-cell subsets recognizing the HIV/SIV viral infectivity factor Vif, may be essential for control of HIV replication as vif,/i>-deficient strains can only complete one round of replication and Vif targets the cellular viral-restriction-factor, APOBEC3G (A3G) with the help of Elongin B/C and Cullin-5 to proteasomal degradation.

HIV-1 HLA-A*0301 restricted epitope KK9, HMYISKKAK (Vif 28-36) does not overlap any known functional domain of Vif.

References

Silver2017 Zachary A. Silver and David I. Watkins. The Role of MHC Class I Gene Products in SIV Infection of Macaques. Immunogenetics, 69(8-9):511-519, Aug 2017. PubMed ID: 28695289. Show all entries for this paper.

Displaying record number 52399

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif(28-36)

Epitope HMYISKKAK Epitope Alignment

Subtype B

Species (MHC/HLA) human(A*0301)

Immunogen HIV-1 infection

Experimental methods

Keywords early-expressed proteins

Notes

CTL responses against regulatory and accessory proteins in 70 HIV-1 infected patients were studied using overlapping peptides and Elispot.

28% targeted one or more overlapping Tat peptides; 36%, Rev peptides; 33%, Vif peptides; 40%, Vpr peptides; and 2%, Vpu peptides.

All known optimally defined epitopes were summarized for the five proteins.

References

Addo2002a M. M. Addo, X. G. Yu, E. S. Rosenberg, B. D. Walker, and M. Altfeld. Cytotoxic T-Lymphocyte (CTL) Responses Directed against Regulatory and Accessory Proteins in HIV-1 Infection. DNA Cell Biol., 21(9):671-678, Sep 2002. PubMed ID: 12396610. Show all entries for this paper.

Displaying record number 55916

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif

Epitope HMYISKKAK Epitope Alignment

Epitope Name HK9(Vif)

Subtype B

Species (MHC/HLA) human(A3)

Immunogen HIV-1 infection

Country China

Experimental methods CD8 T-cell Elispot - IFNγ

Keywords

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-gamma assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.

An inverse correlation was found between CTL response and viral load.

Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.

Previously described HLA-A3-restricted epitope HMYISKKAK elicited an immune response in Chinese HIV-1 positive subjects as part of peptide VKHHMYISKKAKGWLYKH.

1 of the 3 HLA-A3 carriers responded to HMYISKKAK-containing peptidewith a magnitude of CTL response of 70 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 54241

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif

Epitope HMYISKKAK Epitope Alignment

Epitope Name A3-HK9(Vif)

Species (MHC/HLA) human(A3)

Immunogen HIV-1 infection

Experimental methods CD8 T-cell Elispot - IFNγ

Keywords rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contribution of immune responses restricted to individual HLA class I molecules were studied.

The most frequently recognized epitopes also elicited the greatest CTL response.

HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.

HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).

In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contribution of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 52153

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif(28-36)

Epitope HMYISKKAK Epitope Alignment

Epitope Name A3-HK9

Subtype B

Species (MHC/HLA) human(A3)

Immunogen HIV-1 infection

Patient MHC/HLA A3, B7, Cw7

Experimental methods

Keywords dynamics, supervised treatment interruptions (STI), acute/early infection

Notes

CTL responses in 18 acutely HIV-infected HLA-A3 (n=7) or -B7 (n=4) or both -A3 and B7 (n=7) positive individuals were studied.

One individual, AC-06, was homozygous at all three class I alleles (A3, B7, Cw7), was treated during acute infection and had supervised treatment interruptions (STI). He had only two detectable CTL responses during acute infection, but after STI this broadened to 27 distinct epitopes including 15 restricted by HLA-A3, 11 by HLA-B7, and 1 by HLA-Cw7.

0/14 HLA-A3 positive individuals had detectable A3-restricted responses to this epitope during acute infection, but only 5/15 of HLA-A3 epitopes tested were targeted during acute infection. 2/7 individuals had detectable responses to this epitope after STI.

References

Yu2002b Xu G. Yu, Marylyn M. Addo, Eric S. Rosenberg, William R. Rodriguez, Paul K. Lee, Cecily A. Fitzpatrick, Mary N. Johnston, Daryld. Strick, Philip J. R. Goulder, Bruce D. Walker, and Marcus Altfeld. Consistent Patterns in the Development and Immunodominance of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific CD8+ T-Cell Responses Following Acute HIV-1 Infection. J. Virol., 76(17):8690-8701, Sep 2002. PubMed ID: 12163589. Show all entries for this paper.

Displaying record number 59757

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif(28-36)

Epitope HMYISKKAK Epitope Alignment

Epitope Name HK9

Species (MHC/HLA) human(A*6802)

Immunogen HIV-1 infection

Experimental methods CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay

Keywords rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.

HMYISKKAK had a significantly positive association with HLA A*6802; it was recognized by 5/26 A*6802-positive subjects and 5/315 A*6802-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59759

HXB2 Location Vif(28-36)
DNA(5122..5148) Vif Epitope Map

Author Location Vif(28-36)

Epitope HMYISKKAK Epitope Alignment

Epitope Name HK9

Species (MHC/HLA) human(B*5802)

Immunogen HIV-1 infection

Experimental methods CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay

Keywords rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.

HMYISKKAK had a significantly positive association with HLA B*5802; it was recognized by 1/4 B*5802-positive subjects and 0/251 B*5802-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.