Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 62211

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HXB2 Location	Pol(886-895) Integrase(171-180) DNA(4740..4769)	Pol Epitope Map
Author Location	Integrase(171-180)
Epitope	HLKTAVQMAV	Epitope Alignment
Epitope Name	HV10
Subtype	B
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection, vaccine
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, therapeutic vaccine

Vaccine Details

Notes

• This study investigated the efficacy of a therapeutic vaccine designed to boost CTLs and/or induce new CTL responses in HIV-infected individuals. The vaccine differed from other therapeutic vaccines that were previously studied in that it included not only clade B Gag, Pol, and Nef but also Env proteins from multiple clades (A, B, and C) and was followed by a replication-deficient adenovirus type 5 boost. 17 HIV-infected subjects were randomly assigned in a 2:1 ratio to receive vaccine or placebo. While vaccinated individuals exhibited as significantly stronger CTL response and broadened epitope-specific CTL repertoire, no changes were observed in frequency of latent infection or single-copy viral load.
• The epitope that stimulated the largest increase in CTL response was Clade A YI9, YPPIQGVI. All other epitopes elicited an unchanged or only slightly activated response.
• HV10, HLKTAVQMAV, was one of 9/48 vaccine-matched epitopes that were functionally tested. 6/9 of these, including HV10, were used in functional assays. These epitopes were identified in vaccine recipients by apheresis followed by CTL epitope mapping. Gag-specific responses were found to be most increased by vaccination, though HV10 is an Integrase epitope.

References

Casazza2013 Joseph P. Casazza, Kathryn A. Bowman, Selorm Adzaku, Emily C. Smith, Mary E. Enama, Robert T. Bailer, David A. Price, Emma Gostick, Ingelise J. Gordon, David R. Ambrozak, Martha C. Nason, Mario Roederer, Charla A. Andrews, Frank M. Maldarelli, Ann Wiegand, Mary F. Kearney, Deborah Persaud, Carrie Ziemniak, Raphael Gottardo, Julie E. Ledgerwood, Barney S. Graham, Richard A. Koup, and VRC 101 Study Team. Therapeutic Vaccination Expands and Improves the Function of the HIV-Specific Memory T-Cell Repertoire. J. Infect. Dis., 207(12):1829-1840, 15 Jun 2013. PubMed ID: 23482645. Show all entries for this paper.

Displaying record number 52858

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HXB2 Location	Pol(886-895) Integrase(171-180) DNA(4740..4769)	Pol Epitope Map
Author Location	Pol
Epitope	HLKTAVQMAV	Epitope Alignment
Epitope Name	1247
Subtype	multiple
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Patient MHC/HLA	H0014M: A*01, A*02, B*08
Experimental methods	T-cell Elispot
Keywords	binding affinity, computational epitope prediction, novel epitope

Notes

• Epitopes defined using sequence parsing and matching algorithm Conservatrix, and epitope prediction tool EpiMatrix, were shown to be conserved in a broad range of HIV-1 sequences derived from different parts of the world. 31 novel highly conserved HIV-1 epitopes were found, among which four were recognized as promiscuous epitopes and five as MHC supertypes.
• Estimated binding probability for HLKTAVQMAV: 82%

References

DeGroot2003 Anne S. De Groot, Bill Jesdale, William Martin, Caitlin Saint Aubin, Hakima Sbai, Andrew Bosma, Judy Lieberman, Gail Skowron, Fadi Mansourati, and Kenneth H. Mayer. Mapping Cross-Clade HIV-1 Vaccine Epitopes Using a Bioinformatics Approach. Vaccine, 21(27-30):4486-4504, 1 Oct 2003. PubMed ID: 14505932. Show all entries for this paper.