Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 578

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HXB2 Location	Pol(797-804) Integrase(82-89) DNA(4473..4496)	Pol Epitope Map
Author Location	RT(797-804 SF2)
Epitope	GYIEAEVI	Epitope Alignment
Epitope Name	Pol797-8
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods
Keywords

Notes

• Defined using reverse immunogenetics -- 59 HLA-A*2402 binding peptides were predicted by searching for A*2402 anchors in HIV proteins (Tyr at 2, and Phe, Leu or Ile at the C term) -- 53 of the 59 peptides bound A*2402.
• This peptide induced CTL in 1/4 HIV-1+ people tested.
• GYIEAEVI bound to A*2402 weakly, the epitope can be processed in a vaccinia construct and presented -- two specific CTL clones were obtained.

References

IkedaMoore1997 Y. Ikeda-Moore, H. Tomiyama, K. Miwa, S. Oka, A. Iwamoto, Y. Kaneko, and M. Takiguchi. Identification and Characterization of Multiple HLA-A24-Restricted HIV-1 CTL Epitopes: Strong Epitopes Are Derived from V Regions of HIV-1. J. Immunol., 159:6242-6252, 1997. PubMed ID: 9550428. Show all entries for this paper.

Displaying record number 55580

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HXB2 Location	Pol(797-804) Integrase(82-89) DNA(4473..4496)	Pol Epitope Map
Author Location	Pol(797-804 NL-432 or NL-M20A)
Epitope	GYIEAEVI	Epitope Alignment
Epitope Name	Pol797-8
Subtype	B
Species (MHC/HLA)	human(A*24:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*24:02
Country	Japan
Experimental methods	CTL suppression of replication, Chromium-release assay, HLA binding
Keywords

Notes

• To clarify mechanisms of escape mutation accumulation in the population, the Japanese Nef138-10 (RYPLTFGWCF) epitope was studied amongst hemophiliacs and others, to determine replication suppression abilities of both the wild type and 2F (RfPLTFGWCF) mutant virus. This mutant is conserved due to reduced CTL suppression of viral replication, also preventing viral reversion to WT upon transfer to a new host.
• Epitope Pol797-8, GYIEAEVI, was used as a comparison for positive cytolytic activity of epitope-specific HLA-A*2402 clones against target cells prepulsed with corresponding peptide. These clones partially suppressed NL-M20A viral replication.

References

Fujiwara2008 Mamoru Fujiwara, Junko Tanuma, Hirokazu Koizumi, Yuka Kawashima, Kazutaka Honda, Saori Mastuoka-Aizawa, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Different Abilities of Escape Mutant-Specific Cytotoxic T Cells to Suppress Replication of Escape Mutant and Wild-Type Human Immunodeficiency Virus Type 1 in New Hosts. J. Virol., 82(1):138-147, Jan 2008. PubMed ID: 17959671. Show all entries for this paper.