Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 869

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HXB2 Location	gp160(786-794) gp41(275-283) DNA(8580..8606)	gp160 Epitope Map
Author Location	gp41(791-799 LAI)
Epitope	GRRGWEALK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	review

Notes

• Review of HIV CTL epitopes.
• Also: J. Liebermann 1992 and pers. comm. J. Liebermann.

References

McMichael1994 A. J. McMichael and B. D. Walker. Cytotoxic T Lymphocytes Epitopes: Implications for HIV Vaccine. AIDS, 8S:S155-S173, 1994. Comprehensive review summarizing CTL epitopes that have known HLA type and are fine mapped to indicate epitope boundaries. Anchor residues are indicated when known for different HLA restricted epitopes. Includes a summary of the published literature, as well as much work that was in press or submitted for publication. Show all entries for this paper.

Displaying record number 55602

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HXB2 Location	gp160(786-794) gp41(275-283) DNA(8580..8606)	gp160 Epitope Map
Author Location	Env
Epitope	GRRGWEALK	Epitope Alignment
Epitope Name	GK9
Subtype	B
Species (MHC/HLA)	human(B27, B57)
Immunogen	HIV-1 infection
Country	Netherlands
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	computational epitope prediction

Notes

• Peptide prediction programs based on MHC binding, proteasomal cleavage and TAP transport were applied to select novel epitopes absent from the Los Alamos HIV database. 22 novel epitopes were tested by IFN-gamma ELISpot and 19 (11 HLA-B27 restricted, 8 HLA-B57 restricted) induced strong positive responses.
• Magnitude of response generated was similar to that of previously identified epitopes, but the number of individuals responding was lower. This could be explained by less stringent HLA-typing of subjects and perhaps high sequence variability within these novel epitopes.
• GK9, GRRGWEALK, is a known HLA-B27- and HLA-B57-restricted epitope used as a positive control for eliciting CTL IFN-gamma responses.

References

Schellens2008 Ingrid M. M. Schellens, Can Kesmir, Frank Miedema, Debbie van Baarle, and José A. M. Borghans. An Unanticipated Lack of Consensus Cytotoxic T Lymphocyte Epitopes in HIV-1 Databases: The Contribution of Prediction Programs. AIDS, 22(1):33-37, 2 Jan 2008. PubMed ID: 18090389. Show all entries for this paper.

Displaying record number 53029

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HXB2 Location	gp160(786-794) gp41(275-283) DNA(8580..8606)	gp160 Epitope Map
Author Location	gp41(751-759)
Epitope	GRRGWEALK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*27:05)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, CD8 T-cell Elispot granzyme B
Keywords	Th1, characterizing CD8+ T cells

Notes

• Only 20% of CD8+ T-cells produce IFN-gamma and granzyme B simultaneously (Tc1a). Two additional subpopulations of HIV specific CD8 cells are found, each one constituting 30-40% of the CD8 cell pool. One of these (Tc1b) secretes IFN-gamma only, and the other one (Tc1c) secretes GzB only.
• One of seven patients responded to this peptide with GzB producing cells, and a different patient responded with IFN-gamma producing cells.

References

Kleen2004 Thomas O. Kleen, Robert Asaad, Samuel J. Landry, Bernhard O. Boehm, and Magdalena Tary-Lehmann. Tc1 Effector Diversity Shows Dissociated Expression of Granzyme B and Interferon-gamma in HIV Infection. AIDS, 18(3):383-392, 20 Feb 2004. PubMed ID: 15090789. Show all entries for this paper.

Displaying record number 60949

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HXB2 Location	gp160(786-794) gp41(275-283) DNA(8580..8606)	gp160 Epitope Map
Author Location	gp160(786-794)
Epitope	GRRGWEALK	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*27:05)
Immunogen	HIV-1 infection
Patient MHC/HLA	53617: A*02:01, A*02:05, B*27:05, B*49:01, C*02:02, C*07:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

• The extent to which set-point viremia is affected by early HIV-infection T cell responses to either conserved or variable epitopes was studied. 23 ART-naive subjects were characterized for CTL responses for 6 months, postinfection. While most CTL responses (73/123) were targeted against variable HIV-1 epitopes, recognition of conserved epitopes (16/123) was associated with lower set point viremia. This was independent of HLA type and also functional avidity of the CTL response. Most of the conserved responses were targeted to Gag and Pol epitopes.
• GRRGWEALK, restricted by B*2705, was recognized by 1 patient with the restricting allele.

References

Kunwar2013 Pratima Kunwar, Natalie Hawkins, Warren L. Dinges, Yi Liu, Erin E. Gabriel, David A. Swan, Claire E. Stevens, Janine Maenza, Ann C. Collier, James I. Mullins, Tomer Hertz, Xuesong Yu, and Helen Horton. Superior Control of HIV-1 Replication by CD8+ T Cells Targeting Conserved Epitopes: Implications for HIV Vaccine Design. PLoS One, 8(5):e64405, 2013. PubMed ID: 23741326. Show all entries for this paper.