Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 55990

HXB2 Location	Pol(488-496) RT(333-341) DNA(3546..3572)	Pol Epitope Map
Author Location	RT
Epitope	`GQGQWTYQI`	Epitope Alignment
Epitope Name	GI9(RT)
Subtype	B
Species (MHC/HLA)	human(B13)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-gamma assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Previously described HLA-B13-restricted epitope GQGQWTYQI elicited an immune response in Chinese HIV-1 positive subjects as part of peptide GQGQWTYQIYQEPFKNLK.
>2 of the 29 HLA-B13 carriers responded to GQGQWTYQI-containing peptide with average magnitude of CTL response of 160 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 54094

HXB2 Location	Pol(488-496) RT(333-341) DNA(3546..3572)	Pol Epitope Map
Author Location
Epitope	`GQGQWTYQI`	Epitope Alignment
Epitope Name	GI9
Species (MHC/HLA)	human(B*13)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is a B*13 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 54587

HXB2 Location	Pol(488-496) RT(333-341) DNA(3546..3572)	Pol Epitope Map
Author Location	Pol(488-496)
Epitope	`GQGQWTYQI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Patient MHC/HLA	A0301, A3001, B1301, B1402, Cw0602, Cw0802
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	epitope processing, escape, mutation acquisition, viral fitness and reversion, HLA associated polymorphism

Notes

To determine whether HLA-B*13-restricted CTL responses could partially explain low viremic loads in patients, a cohort of chronic C-clade infected subjects was studied. 6 novel B*13-restricted CTL epitopes were defined from both C- and B-clade viruses, 3 of which were within Gag. B*13-restricted CTL responses correlated with lower viremia, with most immunity targeted against Gag epitopes (p15, p17, p24). Gag epitope escape variants may exact a high viral fitness cost, mutations being seen within and around the optimal epitope.

References

Honeyborne2007 Isobella Honeyborne, Andrew Prendergast, Florencia Pereyra, Alasdair Leslie, Hayley Crawford, Rebecca Payne, Shabashini Reddy, Karen Bishop, Eshia Moodley, Kriebashnie Nair, Mary van der Stok, Noel McCarthy, Christine M. Rousseau, Marylyn Addo, James I. Mullins, Christian Brander, Photini Kiepiela, Bruce D. Walker, and Philip J. R. Goulder. Control of Human Immunodeficiency Virus Type 1 Is Associated with HLA-B*13 and Targeting of Multiple Gag-Specific CD8+ T-Cell Epitopes. J. Virol., 81(7):3667-3672, Apr 2007. PubMed ID: 17251285. Show all entries for this paper.

Displaying record number 59735

HXB2 Location	Pol(488-496) RT(333-341) DNA(3546..3572)	Pol Epitope Map
Author Location	Pol(488-496)
Epitope	`GQGQWTYQI`	Epitope Alignment
Epitope Name	GI9
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GQGQWTYQI had a significantly positive association with HLA B*13; it was recognized by 7/15 B*13-positive subjects and 2/326 B*13-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 61227

HXB2 Location	Pol(488-496) RT(333-341) DNA(3546..3572)	Pol Epitope Map
Author Location	RT(333-341)
Epitope	`GQGQWTYQI`	Epitope Alignment
		Show epitope variants
Epitope Name	GI9
Subtype	B
Species (MHC/HLA)	human(B*1302)
Immunogen	HIV-1 infection
Experimental methods	Relative replication capacity assay
Keywords	class I down-regulation by Nef, escape, novel epitope

Notes

Protective HLA-B*13 was studied by following the 10 most common polymorphisms associated with it. Conserved Gag and Nef B*13-restricted epitopes were shown to have fitness costs when mutated and this makes B*13 a highly protective HLA. In addition, escape-substitutions E24Q and Q107R together, but not separately, impair the ability of Nef to downregulate the MHCI molecule. A novel B*13-restricted Gag p24 epitope GQMVHQAI was identified. Also, Gag-K436R is a compensatory mutation to escape GagI437L.
Epitope GI9, GQGQWTYQI, contains the B*13-associated polymorphism Q334N.

References

Shahid2015 Aniqa Shahid, Alex Olvera, Gursev Anmole, Xiaomei T. Kuang, Laura A. Cotton, Montserrat Plana, Christian Brander, Mark A. Brockman, and Zabrina L. Brumme. Consequences of HLA-B*13-Associated Escape Mutations on HIV-1 Replication and Nef Function. J. Virol., 89(22):11557-11571, Nov 2015. PubMed ID: 26355081. Show all entries for this paper.