Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 61550

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HXB2 Location	Pol(809-817) Integrase(94-102) DNA(4509..4535)	Pol Epitope Map
Author Location	Integrase(94-102)
Epitope	GQETAYFLL	Epitope Alignment
Epitope Name	Pol GL9
Subtype	B, CRF01_AE
Species (MHC/HLA)	human(A*02:06)
Immunogen	HIV-1 infection
Patient MHC/HLA	KI-964: A*02:06
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	optimal epitope, chronic infection

Notes

• Polymorphic epitope regions can be targets for cross-clade CTL response. In addition to 6 conserved AE epitopes, 9 epitopes polymorphic between clades AE and B were identified in chronically infected Japanese subjects. This is the first comprehensive study to identify cross-clade CTL by using overlapping HIV-1 peptides.
• There were no significant differences in total magnitude of CTL response between 26 clade A/E-infected and 401 clade B-infected subjects when tested with 11-mers from HIV-B Nef, Gag and Pol. 6 epitopes were novel viz. Gag LL11, Pol SV9, Pol GI9, Gag RV8, Gag AP8 and Gag EG11.
• Epitope Pol Integrase GL9, GQETAYFLL, is conserved between clades B and A/E and was found in 519/1003 Clade B and 52/59 Clade A/E Los Alamos database HIV-1 sequences. It was found in 19/26 A/E-infected subjects of the KI cohort.

References

Watanabe2013 Koji Watanabe, Hayato Murakoshi, Yoshiko Tamura, Madoka Koyanagi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Identification of Cross-Clade CTL Epitopes in HIV-1 Clade A/E-Infected Individuals by Using the Clade B Overlapping Peptides. Microbes Infect., 15(13):874-886, Nov 2013. PubMed ID: 23968885. Show all entries for this paper.

Displaying record number 58116

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HXB2 Location	Pol(809-817) Integrase(94-102) DNA(4509..4535)	Pol Epitope Map
Author Location	Pol
Epitope	GQETAYFLL	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	A*02:01, A*24:03, B*18:01, B*44:02, C*05:01, C*12:03
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• T-cell epitope response specificities were examined in uninfected subjects who received the Gag/Pol/Nef vaccine used in the Step trial. Variable epitopes were detected more frequently than would be expected from an unbiased sampling. The most conserved epitopes were detected at a lower frequency, similar to the frequency reported for responses to natural infection, suggesting potential epitope masking. This may be a mechanism used by the virus in both contexts to escape effective T-cell surveillance. Development of vaccine strategies that can elicit a greater breadth, and towards conserved regions of the genome in particular, are critical requirements for effective T-cell based vaccines.
• All vacinees had responses to the Gag peptide pool, and most also had responses to the Nef and/or Pol pools. Subsequent mapping using 9mer minipools demonstrated that T-cell epitopes were recognized across the length of all 3 proteins. A total of 105 distinct 9mers were detected, although some of these may have been overlapping 9mers representing a single epitope.
• 1 vaccinee recognized 9mer GQETAYFLL.

References

Li2011a Fusheng Li, Adam C. Finnefrock, Sheri A. Dubey, Bette T. M. Korber, James Szinger, Suzanne Cole, M. Juliana McElrath, John W. Shiver, Danilo R. Casimiro, Lawrence Corey, and Steven G Self. Mapping HIV-1 Vaccine Induced T-Cell Responses: Bias Towards Less-Conserved Regions and Potential Impact on Vaccine Efficacy in the Step Study. PLoS One, 6(6):e20479, 2011. PubMed ID: 21695251. Show all entries for this paper.