Search CTL/CD8+ T-Cell Epitope Database

Found 19 matching records:

Displaying record number 59722

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*07; it was recognized by 2/30 B*07-positive subjects and 1/202 B*07-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 60692

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*15)
Immunogen	HIV-1 infection
Patient MHC/HLA	S3:
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	dendritic cells, assay or method development, memory cells

Notes

In general, mature IL-12p70-producing dendritic cells (DC) that are responsive to CD40L and loaded with inactive autologous HIV-1 were found to prime naive CTL to respond to a far broader range of conserved and variable Gag peptides than DC-stimulated memory T cells could. Stimulated memory CTL also had lower anti-CD4 T-cell cytolytic function than primed naive CTL. A novel in vitro model of DC immunotherapy was established.
Subject S3 reacted to autologous Gag epitope GHQAAMQML, part of peptides TMLNTVGGHQAAMQMLKE and TVGGHQAAMQMLKETINE which elicited CTL responses of 267 and 1247 from naive T cells versus 0 SFC/10⁶ cells from memory T cells.

References

Smith2016 Kellie N. Smith, Robbie B. Mailliard, Paolo A. Piazza, Will Fischer, Bette T. Korber, Ronald J. Fecek, Deena Ratner, Phalguni Gupta, James I. Mullins, and Charles R. Rinaldo. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells. MBio, 7(3), 31 May 2016. PubMed ID: 27247230. Show all entries for this paper.

Displaying record number 55993

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9(p24)
Subtype	B
Species (MHC/HLA)	human(B15, B39)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Previously described HLA-B15-restricted epitope GHQAAMQML elicited an immune response as part of peptide NTMLNTVGGHQAAMQMLK. This epitope is also restricted by HLA-B39 and elicited responses as part of the above peptide and peptide GHQAAMQMLKETINEEAA.
2 of the 21 HLA-B15 carriers responded to GHQAAMQML-containing peptide with average magnitude of CTL response of 105 SFC/million PBMC (author communication and Fig.1). No information regarding HLA-B39 reactivity is provided.

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.

Displaying record number 53968

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
GHQAAMQML is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 52559

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24(61-69)
Epitope	`GHQAAMQML`	Epitope Alignment
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53334

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 59714

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*1510; it was recognized by 4/4 B*1510-positive subjects and 26/337 B*1510-negative subjects.

References

Displaying record number 59720

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B35:02, B35:03, B*53:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*3502,B*3503,B*5301; it was recognized by 5/35 B*3502,B*3503,B*5301-positive subjects and 12/281 B*3502,B*3503,B*5301-negative subjects.

References

Displaying record number 59715

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*38)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*38; it was recognized by 4/6 B*38-positive subjects and 22/331 B*38-negative subjects.

References

Displaying record number 52736

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201 IIIB)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B38)
Immunogen	HIV-1 infection
Experimental methods	Chromium-release assay
Keywords

Notes

Three CD8+ CTL cell clones were derived from 2 HIV-1 positive asymptomatic patients, and their epitope specificities and HLA presenting proteins were defined.

References

Kurane2003 Ichiro Kurane, Kim West, Carmelita U. Tuazon, Weilan Zeng, and Francis A. Ennis. Definition of Two New Epitopes on Human Immunodeficiency Virus Type 1 Gag Protein Recognized by Human CD8+ Cytotoxic T Lymphocyte Clones. J. Clin. Virol., 27(1):38-43, May 2003. PubMed ID: 12727527. Show all entries for this paper.

Displaying record number 59719

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*39)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*39; it was recognized by 3/25 B*39-positive subjects and 4/234 B*39-negative subjects.

References

Displaying record number 200

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24(193-201 LAI)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B39)
Immunogen
Experimental methods
Keywords

Notes

Optimal peptide defined by titration.

References

KuranePerCom I. Kurane and K. West. Personal communication, 1998. Show all entries for this paper.

Displaying record number 55198

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location
Epitope	`GHQAAMQML`	Epitope Alignment
Species (MHC/HLA)	human(B39)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	supertype, HLA cross-presentation

Notes

100 HIV-infected subjects were tested for response to optimally-defined CTL epitopes regardless of the subject's HLA type. Half of all detected responses were seen in the absence of the originally reported restricting HLA class I allele, and only 3% of epitopes were recognized exclusively in the presence of their original allele. Statistical and experimental methods were used to define additional HLA alleles associated with the epitopes.
In addition to its known HLA association (B39), 2 additional HLAs (A03, B38) were statistically predicted to be associated with this epitope.

References

Frahm2007b Nicole Frahm, Karina Yusim, Todd J. Suscovich, Sharon Adams, John Sidney, Peter Hraber, Hannah S. Hewitt, Caitlyn H. Linde, Daniel G. Kavanagh, Tonia Woodberry, Leah M. Henry, Kellie Faircloth, Jennifer Listgarten, Carl Kadie, Nebojsa Jojic, Kaori Sango, Nancy V. Brown, Eunice Pae, M. Tauheed Zaman, Florian Bihl, Ashok Khatri, Mina John, Simon Mallal, Francesco M. Marincola, Bruce D . Walker, Alessandro Sette, David Heckerman, Bette T. Korber, and Christian Brander. Extensive HLA Class I Allele Promiscuity among Viral CTL Epitopes. Eur. J. Immunol., 37(9):2419-2433, Sep 2007. PubMed ID: 17705138. Show all entries for this paper.

Displaying record number 201

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24(193-201 LAI)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*39:01)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a B*3901 epitope.

References

Displaying record number 59717

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*42:01)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*4201; it was recognized by 2/7 B*4201-positive subjects and 7/259 B*4201-negative subjects.

References

Displaying record number 59716

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*52)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*52; it was recognized by 5/15 B*52-positive subjects and 17/316 B*52-negative subjects.

References

Displaying record number 59721

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(B*55)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA B*55; it was recognized by 1/2 B*55-positive subjects and 3/232 B*55-negative subjects.

References

Displaying record number 59718

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	Gag(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Epitope Name	GL9
Species (MHC/HLA)	human(C*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GHQAAMQML had a significantly positive association with HLA C*14; it was recognized by 3/15 C*14-positive subjects and 9/266 C*14-negative subjects.

References

Displaying record number 62798

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HXB2 Location	Gag(193-201) p24(61-69) DNA(1366..1392)	Gag Epitope Map
Author Location	p24(193-201)
Epitope	`GHQAAMQML`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 51 single nucleotide mutations were observed in epitope GHQAAMQML (known to bind to B1510 & B3901) with a log transformation of mean fitness score, relative to NL4-3, of -0.6496.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.