Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 59298

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17
Epitope	`GELDRWEKI`	Epitope Alignment
Epitope Name	GI9
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression

Notes

620 patients in early/acute stages of infection were screened for Elispot responses to optimally defined epitopes to find associations between specific CTL responses and long-term control of HIV-1. Seven epitope-specific responses were associated with delayed initiation of ART, and 12 were associated with a more rapid initiation of therapy. While initial viremia, HLA Class I genotype and viral set points were associated, breadth and magnitude of response did not influence early viral set point. A broader CTL response to Gag, however, associates with slower disease progression.
Initial specificity of CTL response rather than restricting HLA-I molecule contributes to disease control.
The epitope GELDRWEKI was associated with delayed initiation of ART.

References

Streeck2014 Hendrik Streeck, Richard Lu, Noor Beckwith, Mark Milazzo, Michelle Liu, Jean-Pierre Routy, Susan Little, Heiko Jessen, Anthony D. Kelleher, Frederick Hecht, Rafick-Pierre Sekaly, Galit Alter, David Heckerman, Mary Carrington, Eric S. Rosenberg, and Marcus Altfeld. Emergence of Individual HIV-Specific CD8 T Cell Responses during Primary HIV-1 Infection Can Determine Long-Term Disease Outcome. J. Virol., 88(21):12793-12801, Nov 2014. PubMed ID: 25165102. Show all entries for this paper.

Displaying record number 59709

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	Gag(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
Epitope Name	GI9
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
GELDRWEKI had a significantly positive association with HLA B*40; it was recognized by 11/28 B*40-positive subjects and 9/313 B*40-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 53844

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
		Show epitope variants
Epitope Name	GI9
Subtype	B
Species (MHC/HLA)	human(B*40)
Immunogen	HIV-1 infection
Patient MHC/HLA	A30, B18, B40, C02, C*05
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	responses in children, mother-to-infant transmission, escape, acute/early infection, viral fitness and reversion

Notes

CD8 T-cell responses were examined in mother-infant pairs. Escape variants were commonly detected in maternal plasma. Early infant plasma viruses showed heterogeneity of gag and nef gene sequences as well as mother-to-child transmission of CD8 T-cell escape variants. The stability of escape mutants in the infant over time was determined by infant HLA haplotype and viral fitness.
Sequential plasma specimens from infants showed changes in CD8 T-cell epitope sequences, suggesting that infants are capable of generating virus-specific CD8 T-cell responses.
The escape variant geldrwKki was detected in 10/10 maternal clones from a B*40-positive mother (M-1002), but was absent in all sequences from her B*40-negative infant (P-1031), sampled at months 2, 4, and 11, suggesting either transmission of a minority wild-type variant, or rapid reversion in the absence of continued CTL pressure.
geldrwKki elicted lower responder cell frequencies than GELDRWEKI.

References

Sanchez-Merino2005 Victor Sanchez-Merino, Siwei Nie, and Katherine Luzuriaga. HIV-1-Specific CD8+ T Cell Responses and Viral Evolution in Women and Infants. J. Immunol., 175(10):6976-6986, 15 Nov 2005. PubMed ID: 16272358. Show all entries for this paper.

Displaying record number 54366

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p15
Epitope	`GELDRWEKI`	Epitope Alignment
Epitope Name	B40-GI9(p15)
Species (MHC/HLA)	human(B40)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contributions of immune responses restricted to individual HLA class I molecules were studied.
This epitope-HLA combination was recognized. The most frequently recognized epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contributions of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 1958

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
Epitope Name	Gag-GI9, p17-GI9
Subtype	B
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	01RCH46: A02:01, A02:17, B08:01, B40:02, C03:03, C07:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	HAART, ART, novel epitope

Notes

This study monitored epitope responses in HIV-1 infected minority women living in the United States.
24 epitopes were described -- 8 were novel, 8 used new restricting elements but were previously defined epitopes, and 8 were previously described.
Serial peptide truncations were used to define optimal epitopes for CTL cell lines isolated from 12 individuals, assayed by a Cr-release.
This epitope was newly defined in this study.
Patient 01RCH46 was Hispanic, on HAART, and had a viral load of 21000 and CD4 count of 623 -- she also recognized KETINEEAA p24(70-78), HLA B*4002, and TAFTIPSI, RT(128-135), HLA A*0217.
Among HIV+ individuals who carried HLA B40, 2/5 (40%) recognized this epitope.

References

Sabbaj2003 Steffanie Sabbaj, Anju Bansal, G. Douglas Ritter, Christopher Perkins, Bradley H. Edwards, Ethan Gough, Jianming Tang, James J. Szinger, Bette Korber, Craig M. Wilson, Richard A. Kaslow, Mark J. Mulligan, and Paul A. Goepfert. Cross-Reactive CD8+ T Cell Epitopes Identified in US Adolescent Minorities. J. Acquir. Immune Defic. Syndr., 33(4):426-438, 1 Aug 2003. PubMed ID: 12869831. Show all entries for this paper.

Displaying record number 52568

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 60944

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*49:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	53617: A02:01, A02:05, B27:05, B49:01, C02:02, C07:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

The extent to which set-point viremia is affected by early HIV-infection T cell responses to either conserved or variable epitopes was studied. 23 ART-naive subjects were characterized for CTL responses for 6 months, postinfection. While most CTL responses (73/123) were targeted against variable HIV-1 epitopes, recognition of conserved epitopes (16/123) was associated with lower set point viremia. This was independent of HLA type and also functional avidity of the CTL response. Most of the conserved responses were targeted to Gag and Pol epitopes.
GELDRWEKI, restricted by B*4901, was recognized by 1 patient with the restricting allele.

References

Kunwar2013 Pratima Kunwar, Natalie Hawkins, Warren L. Dinges, Yi Liu, Erin E. Gabriel, David A. Swan, Claire E. Stevens, Janine Maenza, Ann C. Collier, James I. Mullins, Tomer Hertz, Xuesong Yu, and Helen Horton. Superior Control of HIV-1 Replication by CD8+ T Cells Targeting Conserved Epitopes: Implications for HIV Vaccine Design. PLoS One, 8(5):e64405, 2013. PubMed ID: 23741326. Show all entries for this paper.

Displaying record number 62626

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HXB2 Location	Gag(11-19) p17(11-19) DNA(820..846)	Gag Epitope Map
Author Location	p17(11-19)
Epitope	`GELDRWEKI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 54 single nucleotide mutations were observed in epitope GELDRWEKI (known to bind to B4002) with a log transformation of mean fitness score, relative to NL4-3, of -0.6347.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.