Search CTL/CD8+ T-Cell Epitope Database

Found 15 matching records:

Displaying record number 59685

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(A*26)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA A*26; it was recognized by 5/17 A*26-positive subjects and 27/324 A*26-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 59686

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(A*6802)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA A*6802; it was recognized by 4/22 A*6802-positive subjects and 7/201 A*6802-negative subjects.

References

Displaying record number 54349

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	B15-FY10(Tat)
Species (MHC/HLA)	human(B15)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contribution of immune responses restricted to individual HLA class I molecules were studied.
The most frequently recognised epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contribution of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 59689

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(B*1501)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA B*1501; it was recognized by 4/22 B*1501-positive subjects and 11/223 B*1501-negative subjects.

References

Displaying record number 55078

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat
Epitope	`FQTKGLGISY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, rate of progression, immunodominance

Notes

CTL responses restricted by HLA-B*1503 were analyzed in HIV-1 subtype B and C-infected patients. An association between HLA-B*1503 and reduced viral loads in individuals infected with clade B was found. No such correlation was found in clade C infected subjects inspite of the same immunodominant responses in subjects infected with either subtype. Clade B viral control was associated with several subdominanat CTL epitopes lacking in clade C, suggesting that subdominant responses can contribute to viral control.
FQTKGLGISY of clade C is a potential HLA-B*1503 restricted epitope.

References

Frahm2006 Nicole Frahm, Photini Kiepiela, Sharon Adams, Caitlyn H. Linde, Hannah S. Hewitt, Kaori Sango, Margaret E. Feeney, Marylyn M. Addo, Mathias Lichterfeld, Matthew P. Lahaie, Eunice Pae, Alysse G. Wurcel, Timothy Roach, M Anne. St. John, Marcus Altfeld, Francesco M. Marincola, Corey Moore, Simon Mallal, Mary Carrington, David Heckerman, Todd M. Allen, James I. Mullins, Bette T. Korber, Philip J. R. Goulder, Bruce D. Walker, and Christian Brander. Control of Human Immunodeficiency Virus Replication by Cytotoxic T Lymphocytes Targeting Subdominant Epitopes. Nat. Immunol., 7(2):173-178, Feb 2006. PubMed ID: 16369537. Show all entries for this paper.

Displaying record number 57987

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location
Epitope	`FQTKGLGISY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Country	South Africa, United Kingdom
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	computational epitope prediction, optimal epitope

Notes

A prediction tool, HLArestrictor, was developed to predict minimal epitopes from longer peptides. Minimal epitopes were predicted and validated for 18 EliSpot-positive 18mers.
FQTKGLGISY was predicted from EliSpot-positive 18mer YHCLVCFQTKGLGISYGR. The epitope was validated in one B*1503-positive HIV-positive individual by tetramer staining.

References

ErupLarsen2011 Malene Erup Larsen, Henrik Kloverpris, Anette Stryhn, Catherine K. Koofhethile, Stuart Sims, Thumbi Ndung'u, Philip Goulder, Søren Buus, and Morten Nielsen. HLArestrictor---A Tool for Patient-Specific Predictions of HLA Restriction Elements and Optimal Epitopes within Peptides. Immunogenetics, 63(1):43-55, Jan 2011. PubMed ID: 21079948. Show all entries for this paper.

Displaying record number 59156

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(67-76)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10-Tat
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding, Tetramer binding
Keywords	TCR usage, characterizing CD8+ T cells

Notes

Inhibitory receptor [programmed death-1 (PD-1)/lymphocyte activation gene-3 (LAG-3)/ T-cell immunoglobulin and mucin domain-3 (Tim-3)] expression was studied during chronic infection in 128 CTL populations from 32 ART-naive, sub-Saharan, HIV-clade C-infected subjects expressing either HLA-B*1503 or -B*4201. PD-1 was found to vary across epitope specificity whether inter- or intra-subject. PD-1 also varied with different TCR clonotypes even within one HIV-specific CTL population. PD-1 correlates directly with viral load (VL).
Differential epitope-linked exhaustion marked by inhibitory receptor expression was seen for FY10-Tat, FQTKGLGISY,-specific CTL. These CTL previously associated with lack of protection expressed high CD127 and low PD-1. CTL specific for FY10-Tat, a highly variable epitope, were found to lack significant correlation with VL.

References

Kloverpris2014a Henrik N. Kløverpris, Reuben McGregor, James E McLaren, Kristin Ladell, Anette Stryhn, Catherine Koofhethile, Jacqui Brener, Fabian Chen, Lynn Riddell, Luzzi Graziano, Paul Klenerman, Alasdair Leslie, Søren Buus, David A. Price, and Philip Goulder. Programmed Death-1 Expression on HIV-1-Specific CD8+ T Cells Is Shaped by Epitope Specificity, T-Cell Receptor Clonotype Usage and Antigen Load. AIDS, 28(14):2007-2021, 10 Sep 2014. PubMed ID: 24906112. Show all entries for this paper.

Displaying record number 1281

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	( subtype C)
Epitope	`FQTKGLGISY`	Epitope Alignment
		Show epitope variants
Epitope Name	T38-FY10
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Country	Botswana
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	immunodominance, optimal epitope

Notes

This study is provides a survey of CTL responses and full length HIV-1 genome sequences from a C subtype infected Botswanan cohort.
17 of 46 patient reacted with Tat immunodominant peptide VCFQTKGLGISYGRK.
FQTKGLGISY was the optimal epitope in the peptide VCFQTKGLGISYGRK among B*1503+ individuals.

References

Novitsky2001 V. Novitsky, N. Rybak, M. F. McLane, P. Gilbert, P. Chigwedere, I. Klein, S. Gaolekwe, S. Y. Chang, T. Peter, I. Thior, T. Ndung'u, F. Vannberg, B. T. Foley, R. Marlink, T. H. Lee, and M. Essex. Identification of human immunodeficiency virus type 1 subtype C Gag-, Tat-, Rev-, and Nef-specific elispot-based cytotoxic T-lymphocyte responses for AIDS vaccine design. J. Virol., 75(19):9210-28, Oct 2001. URL: http://jvi.asm.org/cgi/content/full/75/19/9210. PubMed ID: 11533184. Show all entries for this paper.

Displaying record number 52556

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53290

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	( C consensus)
Epitope	`FQTKGLGISY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 54049

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	( C consensus)
Epitope	`FQTKGLGISY`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
FQTKGLGISY is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 59688

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(B*1503)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA B*1503; it was recognized by 2/10 B*1503-positive subjects and 4/190 B*1503-negative subjects.

References

Displaying record number 59690

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(B*1540)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA B*1540; it was recognized by 1/1 B*1540-positive subjects and 6/200 B*1540-negative subjects.

References

Displaying record number 59687

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(C*06)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA C*06; it was recognized by 12/79 C*06-positive subjects and 15/245 C*06-negative subjects.

References

Displaying record number 59684

HXB2 Location	Tat(38-47) DNA(5942..5971)	Tat Epitope Map
Author Location	Tat(38-47)
Epitope	`FQTKGLGISY`	Epitope Alignment
Epitope Name	FY10
Species (MHC/HLA)	human(C*12)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FQTKGLGISY had a significantly positive association with HLA C*12; it was recognized by 3/30 C*12-positive subjects and 1/160 C*12-negative subjects.