Search CTL/CD8+ T-Cell Epitope Database

Found 15 matching records:

Displaying record number 59682

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Epitope Name	FR10
Species (MHC/HLA)	human(A*68:02)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FPVTPQVPLR had a significantly positive association with HLA A*6802; it was recognized by 4/26 A*6802-positive subjects and 6/315 A*6802-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 1786

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Experimental methods
Keywords	rate of progression, acute/early infection

Notes

The CTL response to optimally defined CTL epitopes restricted by HLA class I A and B alleles in individuals who coexpressed HLA A*02, A*03, and B*07 was studied in eight HIV-1-infected subjects, two with acute infection, five with chronic, and one long-term non-progressor (LTNP)
2 to 17 epitopes were recognized in a given individual, A*02-restricted CTL response tended to be narrow and never dominated the response, and 25/27 epitopes were targeted by at least one person.
Subjects with chronic HIV-1 infection recognized between 2-8 out of 11 B*07-restricted epitopes.
An acute seroconvertor homozygous for the B*07 allele recognized five B*07-restricted epitopes.
The other acute seroconvertor failed to recognize any of the 11 B*07-restricted epitopes tested.
The B*07-restricted CTL response was highly variable and there was no clearly dominant epitope.

References

Day2001 C. L. Day, A. K. Shea, M. A. Altfeld, D. P. Olson, S. P. Buchbinder, F. M. Hecht, E. S. Rosenberg, B. D. Walker, and S. A. Kalams. Relative dominance of epitope-specific cytotoxic T-lymphocyte responses in human immunodeficiency virus type 1-infected persons with shared HLA alleles. J. Virol., 75(14):6279-91, Jul 2001. URL: http://jvi.asm.org/cgi/content/full/75/14/6279. PubMed ID: 11413294. Show all entries for this paper.

Displaying record number 53131

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(66-75)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Country	Spain
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Proliferation
Keywords	HAART, ART, supervised treatment interruptions (STI)

Notes

Structured treatment interruption (STI) alone is not able to control viral replication in chronically infected patients, probably due to lack of strong, maintained T-helper cell responses. HIV-1 specific CD8+ T-cell responses were shown to increase significantly until the end of the follow up, but were not correlated with viral load.
Less than 2 of 7 patients recognized this epitope.

References

Plana2004 Montserrat Plana, Felipe Garcia, Annette Oxenius, Gabriel M. Ortiz, Anna Lopez, Anna Cruceta, Gabriel Mestre, Emilio Fumero, Catherine Fagard, Maria Antonia Sambeat, Ferran Segura, José M. Miró, Mireia Arnedo, L. Lopalcos, Tomas Pumarola, Bernard Hirschel, Rodney E. Phillips, Douglas F. Nixon, Teresa Gallart, and Jose M. Gatell. Relevance of HIV-1-Specific CD4+ Helper T-Cell Responses During Structured Treatment Interruptions in Patients With CD4+ T-Cell Nadir Above 400/mm3. J. Acquir. Immune Defic. Syndr., 36(3):791-799, 1 Jul 2004. PubMed ID: 15213562. Show all entries for this paper.

Displaying record number 57532

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
		Show epitope variants
Epitope Name	F10LR
Species (MHC/HLA)	human, transgenic mouse(B*07)
Immunogen	HIV-1 infection, vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, HLA binding
Keywords	vaccine-induced immune responses, variant cross-reactivity, vaccine antigen design

Vaccine Details

Vaccine type	polyepitope
Vaccine strain	multiple epitope immunogen
Vaccine component	HIV-1

Notes

The study objective was to design a vaccine construct containing HIV-1 epitopes that would generate broad CD8+ responses. Among 14 known B7-restricted epitopes, 11 induced a response in B*07 transgenic mice. Among 11 novel candidate epitopes, all induced a response in B*07 mice, and all induced a response in at least 1 human B7+ donor. Immunological recognition of these epitopes was confirmed in HLA-B*0702 HIV-1-infected individuals.
FPVTPQVPLR is a known B07 epitope that was recognized by 6/12 B*07 transgenic mice.
This epitope was recognized by 4/8 HLA-B*0702 HIV-1-infected individuals.
Intersubtype variant FPVrPQVPLR exhibited low cross-recognition in mice immunized with FPVTPQVPLR.

References

Cardinaud2009 Sylvain Cardinaud, Romain Bouziat, Pierre-Simon Rohrlich, Sophie Tourdot, Laurence Weiss, Pierre Langlade-Demoyen, Anne Burgevin, Susana Fiorentino, Peter van Endert, and François A. Lemonnier. Design of a HIV-1-Derived HLA-B07.02-Restricted Polyepitope Construct. AIDS, 23(15):1945-1954, 24 Sep 2009. PubMed ID: 19644347. Show all entries for this paper.

Displaying record number 58528

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B07, B35)
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Other
Keywords	characterizing CD8+ T cells, elite controllers

Notes

Dual color EliSpot testing for IL-2 and IFN-γ was used to determine whether CTL restricted by MHC Class I alleles associated with slow progression to disease, viz. B*57 and B*27, had a more poly-functional profile than other CD8+ cells in a treatment-naive cohort from Montreal. B*57/B*27-restricted CTL producing IL-2 or IFN-γ or IL-2/IFN-γ, were similar in both slow progressors (SP) and progressors. Other non-protective MHC-I-restricted CTL producing both IL-2/IFN-γ, were greater in number in SP than progressors.
Within SP, peptides restricted by both protective and non-protective MHC-I alleles elicited CTL with the same functional profile. For progressors, peptides and epitopes restricted by protective B*57/B*27 alleles stimulated a higher proportion of dual-(IL-2/IFN-γ)secreting CTL.
HLA-B*07/B*35-restricted Nef optimal epitope FPVTPQVPLR was tested in a dual color EliSpot and was found to elicit CTL that could produce IL-2 and/or IFN-γ.

References

Peretz2011 Yoav Peretz, Olivia Marra, Réjean Thomas, Danielle Legault, Pierre Côté, Mohamed-Rachid Boulassel, Danielle Rouleau, Jean-Pierre Routy, Rafick-Pierre Sékaly, Christos M Tsoukas, Cécile Tremblay, and Nicole F. Bernard. Relative Contribution of HIV-Specific Functional Lymphocyte Subsets Restricted by Protective and Non-Protective HLA Alleles. Viral Immunol, 24(3):189-198, Jun 2011. PubMed ID: 21668360. Show all entries for this paper.

Displaying record number 58982

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*07:02)
Immunogen	HIV-1 infection
Country	Australia, Canada, Spain, Switzerland
Experimental methods	Other
Keywords	mutation acquisition

Notes

Computational genome-wide scans were run to find associations between (subjects' ) host genome DNA polymorphisms, and infecting HIV-1 genome sequence variation from 1071 subjects, as well as their viral load (VL). Such genome-to-genome approaches also reveal sites of single nucleotide polymorphisms (SNPs) linked to variant HIV-1 amino acids that either limit viral efficiency or that are easily tolerated by it.
48 amino acid residues of HIV-1 were linked to human SNPs, and the variants were not antiretroviral drug resistance mutations. 50% of these viral amino acids were located within previously defined optimal epitopes, such as FPVTPQVPLR, whose variation at position 71 is associated with SNP rs2596488.

References

Bartha2013 István Bartha, Jonathan M. Carlson, Chanson J. Brumme, Paul J. McLaren, Zabrina L. Brumme, Mina John, David W. Haas, Javier Martinez-Picado, Judith Dalmau, Cecilio López-Galíndez, Concepción Casado, Andri Rauch, Huldrych F. Günthard, Enos Bernasconi, Pietro Vernazza, Thomas Klimkait, Sabine Yerly, Stephen J. O'Brien, Jennifer Listgarten, Nico Pfeifer, Christoph Lippert, Nicolo Fusi, Zoltán Kutalik, Todd M. Allen, Viktor Müller, P. Richard Harrigan, David Heckerman, Amalio Telenti, and Jacques Fellay. A Genome-to-Genome Analysis of Associations between Human Genetic Variation, HIV-1 Sequence Diversity, and Viral Control. eLife, 2:e01123, 2013. PubMed ID: 24171102. Show all entries for this paper.

Displaying record number 965

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77 LAI)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*07:02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a B*0702 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 964

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77 LAI)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

There was a high degree of variation in three CTL epitopes in Nef in four slow and non-progressors, and variant specific CTLs arose over time to eliminate variants, indicating immune selection.

References

Haas1996 G. Haas, U. Plikat, P. Debre, M. Lucchiari, C. Katlama, Y. Dudoit, O. Bonduelle, M. Bauer, H. Ihlenfeldt, G. Jung, B. Maier, A. Meyerhans, and B. Autran. Dynamics of Viral Variants in HIV-1 Nef and Specific Cytotoxic T Lymphocytes In Vivo. J. Immunol., 157:4212-4221, 1996. PubMed ID: 8892659. Show all entries for this paper.

Displaying record number 1321

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef( subtype B)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS), escape

Notes

This study examines CTL responses in HIV exposed, persistently seronegative individuals, HEPS, who eventually seroconverted -- 11/114 HEPS Nairobi sex workers eventually seroconverted, and for six of these HIV CTL reactive epitopes had been defined while seronegative.
FPVTPQVPLR was recognized in 1 of the 6 women (ML1203), and the response was present in the last available sample prior to seroconversion, 7 months.
20/20 sequences of the infecting strain had no substitutions in this epitope, all were FPVTPQVPLR, so there was no evidence for escape.
The epidemiological factor associated with seroconversion was stopping sex work. HIV-specific CTL activity declined when HEPS sex workers stopped working for a period or retired.
This epitope was recognized in 1/22 HEPS sex worker controls, ML851.

References

Kaul2001 R. Kaul, S. L. Rowland-Jones, J. Kimani, T. Dong, H. B. Yang, P. Kiama, T. Rostron, E. Njagi, J. J. Bwayo, K. S. MacDonald, A. J. McMichael, and F. A. Plummer. Late Seroconversion in HIV-Resistant Nairobi Prostitutes Despite Pre-Existing HIV-Specific CD8+ Responses. J. Clin. Invest., 107(3):341-349, Feb 2001. URL: http://www.jci.org/cgi/content/full/107/3/341. PubMed ID: 11160158. Show all entries for this paper.

Displaying record number 1570

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77 SF2)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, acute/early infection

Notes

Therapy provided during acute infection resulted in a narrower CTL response, stronger T help response, and a less diverse viral population than was seen in individuals treated during chronic infection.
The breadth and specificity of the response was determined using ELISPOT by studying 19 individuals with pre-seroconversion therapy (Group 1), 11 individuals with primary infection but post-seroconversion therapy (Group 2), and 10 individuals who responded to HAART given during chronic infection (Group 3), using 259 overlapping peptides spanning p17, p24, RT, gp41, gp120 and Nef.
Previously described and newly defined optimal epitopes were tested for CTL response.
Number of HLA-B7+ individuals that had a CTL response to this epitope broken down by group: 0/4 group 1, 0/3 group 2, and 1/1 group 3.

References

Altfeld2001b M. Altfeld, E. S. Rosenberg, R. Shankarappa, J. S. Mukherjee, F. M. Hecht, R. L. Eldridge, M. M. Addo, S. H. Poon, M. N. Phillips, G. K. Robbins, P. E. Sax, S. Boswell, J. O. Kahn, C. Brander, P. J. Goulder, J. A. Levy, J. I. Mullins, and B. D. Walker. Cellular immune responses and viral diversity in individuals treated during acute and early HIV-1 infection. J. Exp. Med., 193(2):169-80, 15 Jan 2001. URL: http://www.jem.org/cgi/content/full/193/2/169. PubMed ID: 11148221. Show all entries for this paper.

Displaying record number 1670

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and to B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPG(V/I)RYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 52165

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-76)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Patient MHC/HLA	A3, B7, Cw7
Experimental methods
Keywords	dynamics, supervised treatment interruptions (STI), acute/early infection

Notes

CTL responses in 18 acutely HIV-infected HLA-A3 (n=7) or -B7 (n=4) or both -A3 and B7 (n=7) positive individuals were studied.
One individual, AC-06, was homozygous at all three class I alleles (A3, B7, Cw7), was treated during acute infection and had supervised treatment interruptions (STI). He had only two detectable CTL responses during acute infection, but after STI this broadened to 27 distinct epitopes including 15 restricted by HLA-A3, 11 by HLA-B7, and 1 by HLA-Cw7.
0/11 HLA-B7 individuals had detectable B7-restricted responses to this epitope during acute infection -- 10/15 of HLA-B7 epitopes tested were targeted by at least one person during acute infection. Also, none of 4 individuals had detectable responses to this epitope after STI.

References

Yu2002b Xu G. Yu, Marylyn M. Addo, Eric S. Rosenberg, William R. Rodriguez, Paul K. Lee, Cecily A. Fitzpatrick, Mary N. Johnston, Daryld. Strick, Philip J. R. Goulder, Bruce D. Walker, and Marcus Altfeld. Consistent Patterns in the Development and Immunodominance of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific CD8+ T-Cell Responses Following Acute HIV-1 Infection. J. Virol., 76(17):8690-8701, Sep 2002. PubMed ID: 12163589. Show all entries for this paper.

Displaying record number 57177

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location
Epitope	`FPVTPQVPLR`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

To compare the prevalence and immunodominance of CTL responses to conserved and variable epitopes, CTL responses were analyzed in 45 HIV-1 B-infected males during primary and/or chronic infection. Conserved and variable CTL epitopes were recognized with similar probabilities, but conserved epitopes generally elicited subdominant responses. Low viral loads were associated with Gag-specific responses during acute infection, but with Env-specific responses during chronic infection.
Among 5 B7-positive subjects tested during chronic infection, 3 recognized FPVTPQVPLR, and none had an immunodominant response to it.

References

Liu2009c Yi Liu, John McNevin, Morgane Rolland, Hong Zhao, Wenjie Deng, Janine Maenza, Claire E. Stevens, Ann C. Collier, M. Juliana McElrath, and James I. Mullins. Conserved HIV-1 Epitopes Continuously Elicit Subdominant Cytotoxic T-Lymphocyte Responses. J. Infect. Dis., 200(12):1825-1833, 15 Dec 2009. PubMed ID: 19909083. Show all entries for this paper.

Displaying record number 59683

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location	Nef(68-77)
Epitope	`FPVTPQVPLR`	Epitope Alignment
Epitope Name	FR10
Species (MHC/HLA)	human(B*81)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FPVTPQVPLR had a significantly positive association with HLA B*81; it was recognized by 2/9 B*81-positive subjects and 4/306 B*81-negative subjects.

References

Displaying record number 54924

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HXB2 Location	Nef(68-77) DNA(8998..9027)	Nef Epitope Map
Author Location
Epitope	`FPVTPQVPLR`	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	014A-005: A25:01, A30:02, B07:02, B18:01, C07:02, C12:03
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	vaccine-induced immune responses, acute/early infection

Vaccine Details

Vaccine type	canarypox prime with gp120 boost
Vaccine strain	B clade LAI, B clade MN
Vaccine component	Gag-Pol, gp120, gp41

Notes

T cell responses during early infection in individuals previously enrolled in HIV vaccine trials were compared with those of nonvaccinated subjects.
None of the breakthrough infections possessed vaccine-induced T-cell responses preinfection and 85-86% of vaccinees and nonvaccinees with primary infection developed T-cell responses postinfection, indicating that vaccinees' ability to mount T-cell response postinfection is not compromized by previous immunization.
Breakthrough subjects recognized 43 T-cell epitopes, 8 of them novel, and 25% were present in the vaccine.
This epitope was not contained in the vaccine, but the linked vaccinated patient in whom the study was conducted recognized it after developing infection.

References

Horton2006a Helen Horton, Colin Havenar-Daughton, Deborah Lee, Erin Moore, Jianhong Cao, John McNevin, Thomas Andrus, Haiying Zhu, Abbe Rubin, Tuofu Zhu, Connie Celum, and M. Juliana McElrath. Induction of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific T-Cell Responses in HIV Vaccine Trial Participants Who Subsequently Acquire HIV-1 Infection. J. Virol., 80(19):9779-9788, Oct 2006. PubMed ID: 16973582. Show all entries for this paper.