Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 57949

Download this epitope record as JSON.

HXB2 Location	Pol(8-16) Gag/Pol-TF(8-16) DNA(2106..2132)	Pol Epitope Map
Author Location	Gag/Pol(8-16)
Epitope	FPQGKAREF	Epitope Alignment
Epitope Name	FF9
Subtype	A1, B, C
Species (MHC/HLA)	human(B35)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• The study evaluated vaccine-induced T-cell responses in 40 uninfected volunteers. 12 epitopes were targeted. Certain HLA alleles (B27, B57, B35, B14) were preferentially utilized, while other alleles (A02, B07) were rarely utilized. This preference was explained by 4 factors: higher affinity of specific peptides to specific HLAs, higher avidity of certain T-cell receptors, HLA and peptide interactions, and higher surface expression of certain HLAs.
• 3/7 B35-positive vaccinees responded to FPQGKAREF. This epitope sequence is an exact match for 9% of M-clade and 30% of B-clade sequences in the LANL HIV sequence database.

References

Friedrich2011 David Friedrich, Emilie Jalbert, Warren L. Dinges, John Sidney, Alex Sette, Yunda Huang, M. Juliana McElrath, and Helen Horton. Vaccine-Induced HIV-Specific CD8+ T Cells Utilize Preferential HLA Alleles and Target-Specific Regions of HIV-1. J. Acquir. Immune Defic. Syndr., 58(3):248-252, 1 Nov 2011. PubMed ID: 21709567. Show all entries for this paper.

Displaying record number 57798

Download this epitope record as JSON.

HXB2 Location	Pol(8-16) Gag/Pol-TF(8-16) DNA(2106..2132)	Pol Epitope Map
Author Location	Pol
Epitope	FPQGKAREF	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(C*04:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	computational epitope prediction, HLA associated polymorphism

Notes

• From a large study of HLA-associated polymorphisms, 431 putative epitopes with good EpiPred scores were selected; 320 of these were tested for IFNgamma EliSpot responses in subjects carrying the predicted HLA.
• Peptides with response rate >40% (comparable with mean response rate of 33% for known epitopes) and tested in 5 or more individuals with respective HLA were considered in this study as probable novel epitopes and entered in the database. 41 additional "possible" novel epitopes for which either <5 individuals were tested or response rate was below 40%, are not entered in the database.
• There was a markedly higher proportion of putative epitopes in Nef, and the Nef epitopes tended to have the most high-magnitude EliSpot responses. Among 33 epitopes that elicited a response in over 40% out of 5 or more individuals tested, over 61% clustered in Nef.
• Epitope FPQGKAREF was recognized by 3/6 Cw*0401-positive HIV-1-infected patients and was considered in this study to be a "high-probability" novel epitope.

References

Almeida2011 Coral-Ann M. Almeida, Corine Bronke, Steven G. Roberts, Elizabeth McKinnon, Niamh M. Keane, Abha Chopra, Carl Kadie, Jonathan Carlson, David W. Haas, Sharon A. Riddler, Richard Haubrich, David Heckerman, Simon Mallal, and Mina John. Translation of HLA-HIV Associations to the Cellular Level: HIV Adapts to Inflate CD8 T Cell Responses against Nef and HLA-Adapted Variant Epitopes. J. Immunol., 187(5):2502-2513, 1 Sep 2011. PubMed ID: 21821798. Show all entries for this paper.