Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 55527

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HXB2 Location	Pol(155-163) Protease(99)-RT(8) DNA(2547..2573)	Pol Epitope Map
Author Location	Pol(155-163)
Epitope	`FPISPIETV`	Epitope Alignment
Epitope Name	FV9
Subtype	B
Species (MHC/HLA)	human(B*54:01)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	Chromium-release assay, Intracellular cytokine staining
Keywords	optimal epitope

Notes

Asian-expressed HLA-B*5401-restricted epitopes were identified using overlapping-peptide methods and characterized. 5 epitopes from Pol and Nef induced CTL responses that killed target cells in more than 25% of B*5401-carrying tested patients.
7 peptides from Pol and Nef are listed in Fig. 2 as candidates for B*5401 restriction. No Gag-specific epitopes were identified in this study from the patient whose lymphocytes were screened.
FPISPIETV was defined as an optimal epitope for HLA-B*5401 restriction, using truncated peptides.

References

Kitano2008 Mitsutaka Kitano, Naoki Kobayashi, Yuka Kawashima, Tomohiro Akahoshi, Kiyoshi Nokihara, Shinichi Oka, and Masafumi Takighuchi. Identification and Characterization of HLA-B*5401-Restricted HIV-1-Nef and Pol-Specific CTL Epitopes. Microbes Infect, 10(7):7647-72, Jun 2008. PubMed ID: 18538615. Show all entries for this paper.

Displaying record number 57605

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HXB2 Location	Pol(155-163) Protease(99)-RT(8) DNA(2547..2573)	Pol Epitope Map
Author Location	Pol(154-162)
Epitope	`FPISPIETV`	Epitope Alignment
		Show epitope variants
Epitope Name	FV9
Species (MHC/HLA)	human(B*54:01)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	Chromium-release assay, Relative replication capacity assay
Keywords	escape

Notes

Escape mutations were investigated in 5 previously-identified, common to Asian countries HLA-B*5401-restricted HIV-1 CTL epitopes. Escape-associated mutations were found only in FPISPIETV (FV9).
FV9-specific CTLs showed reduced killing activity against target cells pulsed with the FV9-7D mutant (FPISPIdTV) peptide and failed to kill those infected with the FV9-Td mutant virus, suggesting escape.
Longitudinal sequence analysis of FV9 in 2 B*5401-positive HIV-1-infected individuals. The 7D mutant appeared within 4 months of infection and remained stable over 3 years. In 3 B*5401-negative HIV-1-infected individuals who were infected with the 7D mutant, the 7D sequence remained stable during the period tested.
The FV9-7D mutant did not affect viral replication kinetics in vitro.

References

Hashimoto2010 Masao Hashimoto, Mitsutaka Kitano, Kazutaka Honda, Hirokazu Koizumi, Sachi Dohki, Shinichi Oka, and Masafumi Takiguchi. Selection of Escape Mutation by Pol154-162-Specific Cytotoxic T Cells among Chronically HIV-1-Infected HLA-B*5401-Positive Individuals. Hum. Immunol., 71(2):123-127, Feb 2010. PubMed ID: 19891998. Show all entries for this paper.

Displaying record number 58613

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HXB2 Location	Pol(155-163) Protease(99)-RT(8) DNA(2547..2573)	Pol Epitope Map
Author Location	Pol(155-163)
Epitope	`FPISPIETV`	Epitope Alignment
Epitope Name	FV9
Subtype	B
Species (MHC/HLA)	human(B*54:01)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander noted this as an optimal B*5401 epitope based on data from Kitano Microbes and Infection 10 (2008).

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61911

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HXB2 Location	Pol(155-163) Protease(99)-RT(8) DNA(2547..2573)	Pol Epitope Map
Author Location	Pol(155-163)
Epitope	`FPISPIETV`	Epitope Alignment
Epitope Name	FV9
Species (MHC/HLA)	human(B*54:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	KI-115: B54:01; KI-160: B54:01; KI-627: B54:01; KI-119: B54:01; KI-172: B*54:01
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	optimal epitope

Notes

Multiple overlapping peptides across Pol 155-165 can be presented and recognized as well as cross-recognized by CTL. B*5401-restricted CTL from B*5401+ patients recognized any of 9-, 10- 11-mer Pol peptides, with the 9mer, FPISPIETV, being optimal.
FV9 and FP10 are presented by HLA-B*5401. FV11 may also be presented.

References

Hashimoto2012 Masao Hashimoto, Tomohiro Akahoshi, Hayato Murakoshi, Naoki Ishizuka, Shinichi Oka, and Masafumi Takiguchi. CTL Recognition of HIV-1-Infected Cells via Cross-Recognition of Multiple Overlapping Peptides from a Single 11-Mer Pol Sequence. Eur. J. Immunol., 42(10):2621-2631, Oct 2012. PubMed ID: 22740036. Show all entries for this paper.