Search CTL/CD8+ T-Cell Epitope Database

Found 11 matching records:

Displaying record number 1504

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp120(376-384 LAI)
Epitope	`FNCGGEFFY`	Epitope Alignment
Epitope Name	E2
Subtype	B
Species (MHC/HLA)	human(A*29)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

A panel of 16 epitopes covering 15 class I alleles was tested in 14 HIV+ patients from an unselected Caucasian population treated with HAART, using CD8+ cell IFNgamma production to measure responses.
In general, during the first month of treatment viral load decreased and frequencies of HIV-specific CTL tripled and broadened -- eight new HIV specificities that were not previously detectable were newly detected, as were CMV specific CD8+ PBL -- but with continued viral suppression, HIV-specific responses diminished.
Viral rebounds gave different patterns of response: increases or decreases in pre-existing response, new specificities, or no change.

References

Mollet2000 L. Mollet, T. S. Li, A. Samri, C. Tournay, R. Tubiana, V. Calvez, P. Debre, C. Katlama, and B. Autran. Dynamics of HIV-specific CD8+ T lymphocytes with changes in viral load. J. Immunol., 165(3):1692-704, 1 Aug 2000. URL: http://www.jimmunol.org/v165n3/1692/1692-abs-frame.html. PubMed ID: 10903781. Show all entries for this paper.

Displaying record number 59670

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	Env(376-384)
Epitope	`FNCGGEFFY`	Epitope Alignment
Epitope Name	FY9
Species (MHC/HLA)	human(A*29)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FNCGGEFFY had a significantly positive association with HLA A*29; it was recognized by 25/31 A*29-positive subjects and 10/310 A*29-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 60942

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp160(376-384)
Epitope	`FNCGGEFFY`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 infection
Patient MHC/HLA	11439: A01:01, A29:02, B38:01, B44:03, C12:03, C16:01
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

The extent to which set-point viremia is affected by early HIV-infection T cell responses to either conserved or variable epitopes was studied. 23 ART-naive subjects were characterized for CTL responses for 6 months, postinfection. While most CTL responses (73/123) were targeted against variable HIV-1 epitopes, recognition of conserved epitopes (16/123) was associated with lower set point viremia. This was independent of HLA type and also functional avidity of the CTL response. Most of the conserved responses were targeted to Gag and Pol epitopes.
FNCGGEFFY, restricted by A29, was recognized by 1 patient with the restricting allele.

References

Kunwar2013 Pratima Kunwar, Natalie Hawkins, Warren L. Dinges, Yi Liu, Erin E. Gabriel, David A. Swan, Claire E. Stevens, Janine Maenza, Ann C. Collier, James I. Mullins, Tomer Hertz, Xuesong Yu, and Helen Horton. Superior Control of HIV-1 Replication by CD8+ T Cells Targeting Conserved Epitopes: Implications for HIV Vaccine Design. PLoS One, 8(5):e64405, 2013. PubMed ID: 23741326. Show all entries for this paper.

Displaying record number 55121

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp120(376-384)
Epitope	`FNCGGEFFY`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(A29)
Immunogen
Experimental methods
Keywords	subtype comparisons, viral fitness and reversion

Notes

Determined the frequencies of known CD8+ and CD4+ Env epitope sequences among Brazilian HIV sequences of various subtypes.
Present in 145/200 Brazilian HIV sequences; a common variant is FNCrGEFFY.

References

Queiroz2007 Artur Trancoso Lopo de Queiroz, Luciane Amorim Santos, Domingos Ramon Moreau, Tulio de Oliveira, David I. Watkins, Bernardo Galvão-Castro, and Luiz Carlos Junior Alcantara. Identification and Characterization of Previously Described Epitopes in HIV-1 Subtypes B, C, F and BF in Brazil. Braz. J. Infect. Dis., 11(1):27-30, Feb 2007. PubMed ID: 17625722. Show all entries for this paper.

Displaying record number 57217

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	Env
Epitope	`FNCGGEFFY`	Epitope Alignment
Species (MHC/HLA)	(A29)
Immunogen	HIV-1 infection
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	mother-to-infant transmission

Notes

HIV-1 peptide-specific IFN-γ responses were measured over the first year of life for vertically-infected infants. Late-infected infants (between months 1 and 12) had responses that were more rapid and more focused than those infected before 1 month of life.
1/1 infants tested recognized FNCGGEFFY.

References

Lohman-Payne2009 B. Lohman-Payne, J. A. Slyker, B. A. Richardson, C. Farquhar, M. Majiwa, E. Maleche-Obimbo, D. Mbori-Ngacha, J. Overbaugh, S. Rowland-Jones, and G. John-Stewart. Infants with Late Breast Milk Acquisition of HIV-1 Generate Interferon-gamma Responses More Rapidly than Infants with Early Peripartum Acquisition. Clin. Exp. Immunol., 156(3):511-517, Jun 2009. PubMed ID: 19438605. Show all entries for this paper.

Displaying record number 782

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp120(376-384 IIIB)
Epitope	`FNCGGEFFY`	Epitope Alignment
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

This is the optimal peptide for two CTL clones derived from two different donors.
FNCRGEFFY and FNCRGGFFY are major and minor autologous variants in one of the donors, and showed reduced or no stimulatory activity for CTL from the host.
The IIIB form and the form FNCAGEFFY were present in the other donor, and the CTL line had reduced activity with the FNCAGEFFY form relative to the index peptide.

References

Wilson1997 C. C. Wilson, S. A. Kalams, B. M. Wilkes, D. J. Ruhl, F. Gao, B. H. Hahn, I. C. Hanson, K. Luzuriaga, S. Wolinsky, R. Koup, S. P. Buchbinder, R. P. Johnson, and B. D. Walker. Overlapping Epitopes in Human Immunodeficiency Virus Type 1 gp120 Presented by HLA A, B, and C Molecules: Effects of Viral Variation on Cytotoxic T-Lymphocyte Recognition. J. Virol., 71:1256-1264, 1997. CTL clones were isolated from four individuals that interact with the same 25 amino-acid peptide using three different HLA presenting molecules. PubMed ID: 8995649. Show all entries for this paper.

Displaying record number 52732

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp120
Epitope	`FNCGGEFFY`	Epitope Alignment
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 infection
Experimental methods	Intracellular cytokine staining
Keywords	immunodominance, genital and mucosal immunity

Notes

Predefined immunodominant peptide responses were used to compare CD8+ T cells responses in the blood and cervix of 16 HIV+ Kenyan sex workers. Cervical responses were detected in 8/10 women from whom adequate samples could be obtained. The frequency of the CD8+ T cell response in the genital tract was comparable to the blood, with a trend toward being slightly higher.
The immunodominant response was to this epitope in the PBMC of 1/16 patients (Kaul et al. 2001, AIDS, 107:1303).

References

Kaul2003 Rupert Kaul, Paul Thottingal, Joshua Kimani, Peter Kiama, Charles W. Waigwa, Job J. Bwayo, Francis A. Plummer, and Sarah L. Rowland-Jones. Quantitative Ex Vivo Analysis of Functional Virus-Specific CD8 T Lymphocytes in the Blood and Genital Tract of HIV-Infected Women. AIDS, 17(8):1139-1144, May 2003. PubMed ID: 12819514. Show all entries for this paper.

Displaying record number 55027

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location
Epitope	`FNCGGEFFY`	Epitope Alignment
Species (MHC/HLA)	human(A29)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, optimal epitope

Notes

CTL responses were tested for both HIV-1 and EBV derived epitopes restricted by HLA-A, -B and -C alleles. HLA-B alleles more frequently induce detectable responses and that the responses are generally of greater magnitude than those for HLA-A and -C alleles.
The magnitude and frequency of CTL recognition were related to each other and that TCR avidity for HLA/peptide complexes is a more important determinant for magnitude of response than peptide binding affinity to HLA.
EBV response patterns were not significantly altered by HIV coinfection.
Epitope FNCGGEFFY elicited a magnitude of response of 80 SFC with a functional avidity of 0.1nM.

References

Bihl2006 Florian Bihl, Nicole Frahm, Loriana Di Giammarino, John Sidney, Mina John, Karina Yusim, Tonia Woodberry, Kaori Sango, Hannah S. Hewitt, Leah Henry, Caitlyn H. Linde, John V. Chisholm, III, Tauheed M. Zaman, Eunice Pae, Simon Mallal, Bruce D Walker, Alessandro Sette, Bette T. Korber, David Heckerman, and Christian Brander. Impact of HLA-B Alleles, Epitope Binding Affinity, Functional Avidity, and Viral Coinfection on the Immunodominance of Virus-Specific CTL Responses. J. Immunol., 176(7):4094-4101, 1 Apr 2006. PubMed ID: 16547245. Show all entries for this paper.

Displaying record number 52319

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp160
Epitope	`FNCGGEFFY`	Epitope Alignment
Epitope Name	FNC
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, supervised treatment interruptions (STI)

Notes

Using previously defined epitopes Oxenius2000b,Oxenius2001b in an IFNgamma Elispot assay, 13 chronically HIV-1 infected patients were studied over a period including therapy with standard treatment interruptions (STI).
STIs induced increased recognition of CTL epitopes, but there was no correlation between CTL responses with viral rebound rates, plateau viral loads, or clearance rates.

References

Oxenius2002b Annette Oxenius, Angela R. McLean, Marek Fischer, David A. Price, Sarah J. Dawson, Roland Hafner, Christine Schneider, Helen Joller, Bernard Hirschel, Rodney E. Phillips, Rainer Weber, Huldrych F. Günthard, and Swiss HIV Cohort Study Group. Human Immunodeficiency Virus-Specific CD8+ T-Cell Responses Do Not Predict Viral Growth and Clearance Rates During Structured Intermittent Antiretroviral Therapy. J. Virol., 76(20):10169-10176, Oct 2002. PubMed ID: 12239291. Show all entries for this paper.

Displaying record number 1373

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	gp120(376-384)
Epitope	`FNCGGEFFY`	Epitope Alignment
Epitope Name	FNC
Species (MHC/HLA)	human(B8)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, supervised treatment interruptions (STI), immunodominance, acute/early infection

Notes

Patients who started therapy at acute HIV-1 infection (three with sustained therapy, two with limited therapy upon early infection) had strong HIV specific CD4 proliferative responses and were able to maintain a CTL response even with undetectable viral load -- three patients that had delayed initiation of HAART had no HIV specific CD4 proliferative responses and lost their CTL responses when HAART was eventually given and their viral loads became undetectable.
One of the 7/8 study subjects that were HLA B8 recognized this CTL epitope.
Patient SC19(HLA A11/12, B8/44, Cw06/0701, DR3/7, DR52/53, DQ 2/8) had a CTL response to epitopes FLKEKGGL, GEIYKRWII, ACQGVGGPGHK, AVDLSHFLK, and FNCGGEFFY that declined during therapy initiated at day 197.

References

Oxenius2000b A. Oxenius, D. A. Price, P. J. Easterbrook, C. A. O'Callaghan, A. D. Kelleher, J. A. Whelan, G. Sontag, A. K. Sewell, and R. E. Phillips. Early highly active antiretroviral therapy for acute HIV-1 infection preserves immune function of CD8+ and CD4+ T lymphocytes. Proc. Natl. Acad. Sci. U.S.A., 97(7):3382-7, 28 Mar 2000. URL: http://www.pnas.org/cgi/content/full/97/7/3382. PubMed ID: 10737796. Show all entries for this paper.

Displaying record number 59671

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HXB2 Location	gp160(376-384) gp120(376-384) DNA(7350..7376)	gp160 Epitope Map
Author Location	Env(376-384)
Epitope	`FNCGGEFFY`	Epitope Alignment
Epitope Name	FY9
Species (MHC/HLA)	human(C*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
FNCGGEFFY had a significantly positive association with HLA C*14; it was recognized by 4/15 C*14-positive subjects and 6/295 C*14-negative subjects.