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Displaying record number 61537

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HXB2 Location	Gag(211-221) p24(79-89) DNA(1420..1452)	Gag Epitope Map
Author Location	p24(79-89)
Epitope	EWDRVHPVHAG	Epitope Alignment
		Show epitope variants
Epitope Name	Gag p24 EG11-5V
Subtype	CRF01_AE
Species (MHC/HLA)	human(B*40:02)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	subtype comparisons, variant cross-reactivity, optimal epitope, novel epitope, chronic infection

Notes

• Polymorphic epitope regions can be targets for cross-clade CTL response. In addition to 6 conserved AE epitopes, 9 epitopes polymorphic between clades AE and B were identified in chronically infected Japanese subjects. This is the first comprehensive study to identify cross-clade CTL by using overlapping HIV-1 peptides.
• There were no significant differences in total magnitude of CTL response between 26 clade A/E-infected and 401 clade B-infected subjects when tested with 11-mers from HIV-B Nef, Gag and Pol. 6 epitopes were novel viz. Gag LL11, Pol SV9, Pol GI9, Gag RV8, Gag AP8 and Gag EG11.
• Novel clade A/E epitope Gag p24 EG11-5V, EWDRVHPVHAG, was found in 107/1644 Clade B and 149/315 Clade A/E Los Alamos database HIV-1 sequences. It was found in 5/26 A/E-infected subjects of the KI cohort. CTL induced by this clade B peptide were able to recognize clade B EG11 more strongly than clade A/E peptide EG11.
• CTL from A/E-infected subjects stimulated by the consensus B EG11 peptide used cross-recognized both clades B and A/E infected cells.

References

Watanabe2013 Koji Watanabe, Hayato Murakoshi, Yoshiko Tamura, Madoka Koyanagi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Identification of Cross-Clade CTL Epitopes in HIV-1 Clade A/E-Infected Individuals by Using the Clade B Overlapping Peptides. Microbes Infect., 15(13):874-886, Nov 2013. PubMed ID: 23968885. Show all entries for this paper.