Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 59652

HXB2 Location	gp160(831-838) gp41(320-327) DNA(8715..8738)	gp160 Epitope Map
Author Location	Env(831-838)
Epitope	EVAQRAYR	Epitope Alignment
Epitope Name	ER8
Species (MHC/HLA)	human(A*29)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

• Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
• EVAQRAYR had a significantly positive association with HLA A*29; it was recognized by 3/31 A*29-positive subjects and 4/310 A*29-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 52289

HXB2 Location	gp160(831-838) gp41(320-327) DNA(8715..8738)	gp160 Epitope Map
Author Location	Env(830-837)
Epitope	EVAQRAYR	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*3303)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• HLA-A33 a very common allele in Asia, with HLA-A*3303 the most common among the Japanese. New A*3303 epitopes were defined to better characterize the immune response in this population.
• The anchor motif for HLA*3303 (A, I, L, V, F, Y in position 2 (F and Y bind most strongly), and R (K is also tolerated) in the C-terminal position) was used to define 82 potentially reactive peptides in Env; 37/82 peptides bound to A*3303; 3/37 peptides could induce peptide-specific CTL in bulk PBMC cultures from 1/3 HLA A*3303 positive individuals tested.
• 2/3 peptides that reacted with the bulk culture, EVAQRAYR and VIEVAQRAYR, were overlapping, with one encompassing the other, but EVAQRAYR was shown to be the one that was reactive with a CTL clone.
• CTL clones were isolated that killed target cells in a concentration dependent manner after pulsing with the EVAQRAYR peptide, that could also kill cells transfected with env expressed from a vaccinia vector. Bulk cultures were tested from six additional people, and only 2/6 reacted with this peptide, but the peptide is in a highly variable region.

References

Hossain2001 Mohammad Sohrab Hossain, Hiroko Tomiyama, Takabumi Inagawa, Busarawan Sriwanthana, Shinichi Oka, and Masafumi Takiguchi. HLA-A*3303-Restricted Cytotoxic T Lymphocyte Recognition for Novel Epitopes Derived from the Highly Variable Region of the HIV-1 Env Protein. AIDS, 15(16):2199-2201, 9 Nov 2001. PubMed ID: 11684944. Show all entries for this paper.

Takiguchi2000 M. Takiguchi, T. Matsuda, H. Tomiyama, and K. Miwa. Analysis of three HLA-A*3303 binding peptide anchors using an HLA-A*3303 stabilization assay. Tissue Antigens, 55(4):296-302, Apr 2000. PubMed ID: 10852380. Show all entries for this paper.

Displaying record number 52543

HXB2 Location	gp160(831-838) gp41(320-327) DNA(8715..8738)	gp160 Epitope Map
Author Location	gp41(320-327)
Epitope	EVAQRAYR	Epitope Alignment
Species (MHC/HLA)	human(A*3303)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 59653

HXB2 Location	gp160(831-838) gp41(320-327) DNA(8715..8738)	gp160 Epitope Map
Author Location	Env(831-838)
Epitope	EVAQRAYR	Epitope Alignment
Epitope Name	ER8
Species (MHC/HLA)	human(B*08)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

• Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
• EVAQRAYR had a significantly positive association with HLA B*08; it was recognized by 2/24 B*08-positive subjects and 2/286 B*08-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.