Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 52200

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp41
Epitope	`ERYLRDQQL`	Epitope Alignment
Species (MHC/HLA)	human(B*14)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

Neisseria gonorrhea cervititis in 9 HIV+ Kenyan sex workers caused a functional deficiency in IFN-gamma production in HIV-1 epitope-specific CD8+ T-cells, detected by intracellular cytokine production and tetramer assays, while not affecting the total number of epitope-specific CTLs.
Ghonorrhea caused the weaker HIV-1 specific CTL responses in 4 HIV-1 exposed persistently seronegative (HEPS) women to become undetectable by Elispot and tetramer assays, and CMV-specific CTL in 2 HEPS subjects were shown to have impaired function with regard to IFN-gamma production.

References

Kaul2002 Rupert Kaul, Sarah L. Rowland-Jones, Geraldine Gillespie, Joshua Kimani, Tao Dong, Peter Kiama, J. Neil Simonsen, Job J. Bwayo, Andrew J. McMichael, and Francis A. Plummer. Gonococcal Cervicitis is Associated with Reduced Systemic CD8+ T Cell Responses in Human Immunodeficiency Virus Type 1-Infected and Exposed, Uninfected Sex Workers. J. Infect. Dis., 185(10):1525-1529, 15 May 2002. PubMed ID: 11992292. Show all entries for this paper.

Displaying record number 60937

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp160(584-592)
Epitope	`ERYLRDQQL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*14)
Immunogen	HIV-1 infection
Patient MHC/HLA	94153: A25:01, A68:01, B14:02, B44:02, C05:01, C08:02
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	acute/early infection

Notes

The extent to which set-point viremia is affected by early HIV-infection T cell responses to either conserved or variable epitopes was studied. 23 ART-naive subjects were characterized for CTL responses for 6 months, postinfection. While most CTL responses (73/123) were targeted against variable HIV-1 epitopes, recognition of conserved epitopes (16/123) was associated with lower set point viremia. This was independent of HLA type and also functional avidity of the CTL response. Most of the conserved responses were targeted to Gag and Pol epitopes.
ERYLRDQQL, restricted by B*14, was recognized by 1 patient with the restricting allele.

References

Kunwar2013 Pratima Kunwar, Natalie Hawkins, Warren L. Dinges, Yi Liu, Erin E. Gabriel, David A. Swan, Claire E. Stevens, Janine Maenza, Ann C. Collier, James I. Mullins, Tomer Hertz, Xuesong Yu, and Helen Horton. Superior Control of HIV-1 Replication by CD8+ T Cells Targeting Conserved Epitopes: Implications for HIV Vaccine Design. PLoS One, 8(5):e64405, 2013. PubMed ID: 23741326. Show all entries for this paper.

Displaying record number 1677

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp41(589-597)
Epitope	`ERYLRDQQL`	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 exposed seronegative, HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.

References

Kaul2001a R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420. Show all entries for this paper.

Displaying record number 56649

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	Env(584-592)
Epitope	`ERYLRDQQL`	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B*14:01)
Immunogen	HIV-1 infection
Patient MHC/HLA	A01:01, A23:01, B14:02, B57:01, C07:01, C08:02
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	escape, acute/early infection

Notes

Using overlapping 15-mer peptides, CTL responses to early, acute HIV infection were determined. Early virus escape attributed to CTL response was charted and subsequent clusters of mutations due to NAb targeting were also found.
Five findings made: (i) exact numbers of transmitted HIV-1 actual and potential T cell epitopes were determined (ii) CTL-mediated virus escape occurring as viremia peaks, drops and reaches set point earlier and faster than previously known (iii) CTL responses to some transmitted virus epitopes were lost after early escapes were selected (iv) a few unexplained escapes were probably due to bystander mutations (v) earliest CTL responses cause the decline of VL in acute infection.
This transmitted epitope, ERYLRDQQL which is part of tested peptide sequence LALERYLRDQQLLGIW, elicited an EliSpot response in patient CH58. HLA-restriction was as found in database.
Escape mutations were ERYLgDQQL, ERhLRDQQL, ERYLkDQQL and ERYLsDQQL. Response to mutant peptides was not detected. Escape from this epitope precedes escape from B*5701-restricted TW10, which goes from a subdominant to immunodominant response.

References

Goonetilleke2009 Nilu Goonetilleke, Michael K. P. Liu, Jesus F. Salazar-Gonzalez, Guido Ferrari, Elena Giorgi, Vitaly V. Ganusov, Brandon F. Keele, Gerald H. Learn, Emma L. Turnbull, Maria G Salazar, Kent J Weinhold, Stephen Moore, CHAVI Clinical Core B, Norman Letvin, Barton F. Haynes, Myron S. Cohen, Peter Hraber, Tanmoy Bhattacharya, Persephone Borrow, Alan S. Perelson, Beatrice H. Hahn, George M. Shaw, Bette T. Korber, and Andrew J. McMichael. The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection. J. Exp. Med., 206(6):1253-1272, 8 Jun 2009. PubMed ID: 19487423. Show all entries for this paper.

Displaying record number 58235

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	Env(584-592)
Epitope	`ERYLRDQQL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*14:02)
Immunogen	HIV-1 infection
Patient MHC/HLA	CH058: A01:01, A23:01, B14:02, B57, C07:01, C08:02
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	dynamics, escape, acute/early infection, autologous responses, characterizing CD8+ T cells

Notes

Multifunctionality of CTL against transmitted or founder HIV-1 isolates during early or acute infection was examined for correlation with escape mutation selection. The earliest CTL responses were oligofunctional, and progressively became more multifunctional. A significant association was found between total CTL response and order of escape selection, and between epitope entropy with the rate of emergence of escape mutants.
18-mer LALERYLRDQQLLGIW (Env 581-596) contains epitope ERYLRDQQL, that shows early evidence of escape or variation. This epitope elicited CTL response in patient CH58.

References

Ferrari2011 Guido Ferrari, Bette Korber, Nilu Goonetilleke, Michael K. P. Liu, Emma L. Turnbull, Jesus F. Salazar-Gonzalez, Natalie Hawkins, Steve Self, Sydeaka Watson, Michael R. Betts, Cynthia Gay, Kara McGhee, Pierre Pellegrino, Ian Williams, Georgia D. Tomaras, Barton F. Haynes, Clive M. Gray, Persephone Borrow, Mario Roederer, Andrew J. McMichael, and Kent J. Weinhold. Relationship between Functional Profile of HIV-1 Specific CD8 T Cells and Epitope Variability with the Selection of Escape Mutants in Acute HIV-1 Infection. PLoS Pathog., 7(2):e1001273, 2011. PubMed ID: 21347345. Show all entries for this paper.

Displaying record number 53808

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp160
Epitope	`ERYLRDQQL`	Epitope Alignment
		Show epitope variants
Subtype	A, B, C, D
Species (MHC/HLA)	human
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	A02:02, A74:01, B15:03, B58:02, C02:02, C06:02; A02:01, A30:09, B45:01, B58:02, C02:02, C16:01
Country	Kenya
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	subtype comparisons, variant cross-reactivity

Vaccine Details

Vaccine type	vaccinia
Vaccine strain	A clade, B clade, D clade NDK, C clade consensus
Vaccine component	Env

Notes

Interclade cross-reactivity of clades A, B, C, and D was tested using recombinant vaccinia-based IFN-gamma Elispot assay. 47/74 women had a positive Elispot response to at least one clade, and cross-clade responses were frequent. Clade A responses were most frequent, as expected, as clade A dominates the Kenyan epidemic (85% of the 47 women responded to clade A Env, 74% to B, 62% to C, and 43% to D). Cross-clade CD8 T-cell responses were common and directed at conserved epitopes.
There was a greater magnitude of response to A clade peptides in individuals who responded to more than 1 clade; a 2-fold higher response was observed in clade A in 36% (9/25) of these individuals, and the response to A peptides was never lower. ERYLRDQQL responses were detected in 2 women who had Env responses to all 4 clades, and clade A gave the highest responses; a ERYLkDQQL variant was in clade B and C, and the clade D Env carried ERsLkDQQL. The epitope VSGFLALAW was also recognized by 1 of the women.
HLA-B*5802 was the only HLA common to both women who reacted with ERYLRDQQL, so may be the presenting allele.

References

McKinnon2005 Lyle R. McKinnon, T. Blake Ball, Joshua Kimani, Charles Wachihi, Lucy Matu, Ma Luo, Joanne Embree, Keith R. Fowke, and Francis A. Plummer. Cross-Clade CD8+ T-Cell Responses With a Preference for the Predominant Circulating Clade. J. Acquir. Immune Defic. Syndr., 40(3):245-249, 1 Nov 2005. PubMed ID: 16249696. Show all entries for this paper.

Displaying record number 61062

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HXB2 Location	gp160(584-592) gp41(73-81) DNA(7974..8000)	gp160 Epitope Map
Author Location	gp41(73-81)
Epitope	`ERYLRDQQL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CH058: A01:01, A23:01, B14:02, B57, C07:01, C08:02
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
Epitope ERYLRDQQL had a fitness cost ΔE of -0.73 and T cell response was first detected to it at 21d, escape time was 1 days.

References

Barton2016 John P. Barton, Nilu Goonetilleke, Thomas C. Butler, Bruce D. Walker, Andrew J. McMichael, and Arup K. Chakraborty. Relative Rate and Location of Intra-Host HIV Evolution to Evade Cellular Immunity Are Predictable. Nat. Commun., 7:11660, 23 May 2016. PubMed ID: 27212475. Show all entries for this paper.