Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 54033

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HXB2 Location	Pol(872-881) Integrase(157-166) DNA(4698..4727)	Pol Epitope Map
Author Location	( C consensus)
Epitope	`ELKKIIGQVR`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*33)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
ELKKIIGQVR is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 58650

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HXB2 Location	Pol(872-881) Integrase(157-166) DNA(4698..4727)	Pol Epitope Map
Author Location	Integrase(157-166 NL4-3)
Epitope	`ELKKIIGQVR`	Epitope Alignment
		Show epitope variants
Epitope Name	ER10
Subtype	B
Species (MHC/HLA)	human(A*33)
Immunogen	HIV-1 infection
Country	Australia, Canada, Germany, United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Relative replication capacity assay
Keywords	acute/early infection, HLA associated polymorphism

Notes

The in vitro replicative capacity (RC) of recombinant viruses expressing HIV-1-patient-derived Integrase (Int) mutant sequences during early and chronic infections was examined, and found to correlate with an HLA-I allele hitherto not considered to be protective. Integrase polymorphisms significantly linked to lower RC as well as significantly associated with host HLA expression were S119R (C*05), G163E (A*33-epitope ER10), and I220L (B*52), but no epitopes were known to encompass S119R or I220L.
ELKKIIGQVR, ER10, is restricted by HLA-A*33 and elicits a CTL response.
The S119R polymorphism exhibited an ˜35% lower RC than parental NL4-3, but a compensatory mutation, A91E restored RC to wild-type levels.

References

Brockman2012 Mark A. Brockman, Denis R. Chopera, Alex Olvera, Chanson J. Brumme, Jennifer Sela, Tristan J. Markle, Eric Martin, Jonathan M. Carlson, Anh Q. Le, Rachel McGovern, Peter K. Cheung, Anthony D. Kelleher, Heiko Jessen, Martin Markowitz, Eric Rosenberg, Nicole Frahm, Jorge Sanchez, Simon Mallal, Mina John, P. Richard Harrigan, David Heckerman, Christian Brander, Bruce D. Walker, and Zabrina L. Brumme. Uncommon Pathways of Immune Escape Attenuate HIV-1 Integrase Replication Capacity. J. Virol., 86(12):6913-6923, Jun 2012. PubMed ID: 22496233. Show all entries for this paper.

Displaying record number 55349

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HXB2 Location	Pol(872-881) Integrase(157-166) DNA(4698..4727)	Pol Epitope Map
Author Location	Integrase
Epitope	`ELKKIIGQVR`	Epitope Alignment
Epitope Name	ER9
Species (MHC/HLA)	human(A*33:01)
Immunogen
Experimental methods
Keywords

Notes

E169D is a processing mutation for HLA-B*0702 restricted SPAIFQSSM (SM9) as well as an epitope variation for HLA-A*0301 restricted MTKILEPFR (MR9).
CTL recognition of Int epitope ELKKIIGQVR was not detected post-infection with either wild type (169E) or mutant 169D HIV-1. ER9 was used as a negative control since Jurkat cell line E6-1 is negative for ER9-restricting HLA-A*3301.

References

Zimbwa2007 Peter Zimbwa, Anita Milicic, John Frater, Thomas J. Scriba, Antony Willis, Philip J. R. Goulder, Tilly Pillay, Huldrych Gunthard, Jonathan N. Weber, Hua-Tang Zhang, and Rodney E. Phillips. Precise Identification of a Human Immunodeficiency Virus Type 1 Antigen Processing Mutant. J. Virol., 81(4):2031-2038, Feb 2007. PubMed ID: 17108020. Show all entries for this paper.

Displaying record number 61551

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HXB2 Location	Pol(872-881) Integrase(157-166) DNA(4698..4727)	Pol Epitope Map
Author Location	Integrase(157-166)
Epitope	`ELKKIIGQVR`	Epitope Alignment
Epitope Name	Pol ER10
Subtype	B, CRF01_AE
Species (MHC/HLA)	human(A*33:03)
Immunogen	HIV-1 infection
Patient MHC/HLA	KI-632: A24:02, A33:03
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	optimal epitope, chronic infection

Notes

Polymorphic epitope regions can be targets for cross-clade CTL response. In addition to 6 conserved AE epitopes, 9 epitopes polymorphic between clades AE and B were identified in chronically infected Japanese subjects. This is the first comprehensive study to identify cross-clade CTL by using overlapping HIV-1 peptides.
There were no significant differences in total magnitude of CTL response between 26 clade A/E-infected and 401 clade B-infected subjects when tested with 11-mers from HIV-B Nef, Gag and Pol. 6 epitopes were novel viz. Gag LL11, Pol SV9, Pol GI9, Gag RV8, Gag AP8 and Gag EG11.
Epitope Pol Integrase ER10, ELKKIIGQVR, is conserved between clades B and A/E and was found in 770/1003 Clade B and 40/59 Clade A/E Los Alamos database HIV-1 sequences. It was found in 21/26 A/E-infected subjects of the KI cohort.

References

Watanabe2013 Koji Watanabe, Hayato Murakoshi, Yoshiko Tamura, Madoka Koyanagi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Identification of Cross-Clade CTL Epitopes in HIV-1 Clade A/E-Infected Individuals by Using the Clade B Overlapping Peptides. Microbes Infect., 15(13):874-886, Nov 2013. PubMed ID: 23968885. Show all entries for this paper.

Displaying record number 61856

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HXB2 Location	Pol(872-881) Integrase(157-166) DNA(4698..4727)	Pol Epitope Map
Author Location
Epitope	`ELKKIIGQVR`	Epitope Alignment
Epitope Name	ER10
Subtype	B
Species (MHC/HLA)	human(A*33:03)
Immunogen	HIV-1 infection, vaccine
Patient MHC/HLA	KI-1427: A*33:03
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses

Notes

The candidate tHIVconsvX vaccine is composed of 6 conserved Gag and Pol regions. Specific T cells - from 200 ART-naive, infected Japanese subjects previously exposed to the tHIVconsvX vaccine - that have the ability to supress HIV-1 replication were identified as recognizing 6 Pol epitopes. 6/25 epitopes (comprising 20 known and 5 novel mapped epitopes) remain associated with positive clinical outcome.
A*3303-restricted Pol epitope ELKKIIGQVR was found in 15-mer peptide pairs C342/343. While 14/221 Japanese subjects responded to the epitope, only 2/17 HLA-carrying tested responders reacted to this epitope in EliSpot. ER10-specific CTL can strongly suppress HIV-1 replication in vivo. CTL response to A*3303-restricted ER10 is significantly associated with clinical outcome and found in A*3303-carrying subjects.
243/299 (81.3%) of chronically infected Japanese HIV-1 patients carried this conserved peptide epitope, ELKKIIGQVR (ER10). Thus CTL specific for ER10 can recognize >80% of the circulating HIV-1 in Japan.

References

Zou2019 Chengcheng Zou, Hayato Murakoshi, Nozomi Kuse, Tomohiro Akahoshi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, Tomáš Hanke, and Masafumi Takiguchi. Effective Suppression of HIV-1 Replication by Cytotoxic T Lymphocytes Specific for Pol Epitopes in Conserved Mosaic Vaccine Immunogens. J. Virol., 93(7), 1 Apr 2019. PubMed ID: 30674626. Show all entries for this paper.